The invention discloses a liquid traditional Chinese medicine for preoperative hand disinfection and a preparation method thereof, which belong to the field of traditional Chinese medicines. Raw materials of active ingredients of the liquid traditional Chinese medicine disclosed by the invention include folium sennae, nidus vespae, convexutricle sedge herbs, hibiscus rosa-sinensis, cirsium japonicum, cajeputtree bark, semen arecae, incised spleenwort, macleaya cordata, juniper berry, calcined oyster shell, alocasia cucullata, japanese banana leaf, flat-stemmed tetrastigma, scutellariae barbatae, and all-grass of tomentose corydalis. According to the invention, high-quality medicinal materials are selected and reasonably combined, so that the liquid traditional Chinese medicine conforms to the theories of traditional Chinese medicine and pharmacology and modern medicine and pharmacology, has the effects of clearing away heat and toxic materials, diminishing inflammation and fighting bacteria, and has a significant effect on preoperative hand disinfection; and the liquid traditional Chinese medicine is good in stability, easily soluble in water, non-irritant and low in cost, has no toxic or side effect, and can be widely popularized and applied in preoperative hand disinfection, therefore, the liquid traditional Chinese medicine is an ideal liquid traditional Chinese medicine for hand disinfection.