The invention relates to a robust adaptive anti-interference control method which is applied in a robust adaptive anti-interference control system. The control system comprises a controlled object, areference model, an extended state observer ESO, an adaptive mechanism, an input adaptive adjustor and a feedback adaptive regulator. The method comprises a step of performing feedback compensation byusing an ESO estimating system, and a step of constructing a state error xm-z between a reference model and an ESO observer, combining a robust adaptive design method and obtaining adaptive laws based on an input adaptive regulator adjustment parameter K and a feedback adaptive regulator adjustment parameter F such that all signals in a closed-loop dynamic system are uniformly bounded, wherein atracking e=xm-z, an observation error e<->=Xp-Z and parameters K and F are included, and the adaptive laws comprises an adaptive control law and an adaptive adjustment law. According to the method ofthe present invention, external interference can be actively observed and compensated, and the influence of noise on the system can be reduced.