The invention relates to a preparation method of konjac glucomannan eye masks, which comprises the steps of: preparing konjac glucomannan mixed liquid, preparing mask liquid and preparing the masks. In major raw materials, the konjac glucomannan has excellent biological activity and rheological properties and has the effects of preventing the skin from losing water and slowing down the aging, soyprotein isolate comprises various kinds of essential amino acids for human body and trace elements and has high nutritional value and health care effect, olive oil contains rich squalene with excellent skin affinity and essential fatty acid for human body, can be fast absorbed and has the effect of effectively maintaining the skin elasticity and the glossy, beewax belongs to a pure natural moistening agent and can form a protection film on the surface of the skin, collagen consists of 19 kinds of amino acids and has the effects of moisture preservation, nourishment, skin firming, wrinkle prevention and the like, glycerol realizes the effect of improving the moisture preservation effect, and monoglyceride realizes an emulsification effect. The konjac glucomannan eye masks have the advantages that the moisture is preserved, the wrinkles are prevented, the konjac glucomannan eye masks are natural and do not have side effect, and the like.