32results about How to "The spectrum is clear" patented technology

The invention discloses an extraction and dielectrophoresis analytical method for total protein of cattail cauloid, comprising the following steps of: firstly, efficiently extracting protein from the cattail cauloid by screening through many experiments; under a preferred condition, separating the protein components from the secondary metabolite (such as pigments, phenols, quinones and the like) components by a dielectrophoresis method; and performing qualitative analysis by a silver nitrate staining method under a preferred condition, and / or performing qualitative and quantitative analysis on the separated protein by a mass spectrometer. Shown by experimental results, more protein spots are obtained by separation through the dielectrophoresis analytical method, and detected by PDQuest image analysis software, the number of the protein spots is more than 1000, and the spectrum is clear without transverse and longitudinal stripes; and the method has good repeatability and stability, can be used for conveniently identifying the authenticity of the pharmaceutical / food resource plant cattail, and provides a quality control standard and scientific base for in-depth study, scientific planting, culture, development and utilization of cattail.

The invention relates to a method for constructing an HPLC finger-print spectrum of ice wine, in particular to a method for constructing an HPLC finger-print spectrum of Wunvshan ice wine, and belongs to the technical field of foodstuff analysis. According to the technical scheme, the method comprises the steps of distilling the ice wine, collecting fraction from the initial boiling point to 102 DEG C, and constructing the finger-print spectrum with the HPLC. Compared with the prior art, the method for constructing the finger-print spectrum has the advantages that the method is simple and fast, organic solvent is not needed to be added for extraction, the finger-print spectrum is high in accuracy, good in repeatability, clear and legible, great differentiation is achieved, and the method can be widely applied to quality discrimination of the Wunvshan ice wine.

The invention relates to a construction method of a GC-MS (Gas Chromatography-Mass Spectrometer) fingerprint chromatogram of ice wine, in particular to the construction method of the GC-MS (Gas Chromatography-Mass Spectrometer) fingerprint chromatogram of Wu Nu Mountain ice wine, belonging to the technical field of food analysis. The method is characterized by comprising the following steps of distilling the ice wine, collecting fractions with an initial boiling point of -102DEG C; taking steam of the fractions by using a headspace bottle method; constructing the fingerprint chromatogram by using the GC-MS. Compared with the prior art, the construction method for the fingerprint chromatogram, disclosed by the invention, has the beneficial effects of simplicity, quickness and no need of adding an organic solvent for extracting; the fingerprint chromatogram is high in accuracy, good in repeatability, clear and discernible in chromatogram and great in difference, and can be widely applied to mass discrimination of the Wu Nu Mountain ice wine.

The invention provides a method for detecting differential protein produced by action between alpinetin or cardamonin and serum. The method comprises the following steps: (1) shaking up serum protein and alpinetin or cardamonin with the concentration of 1.0*10<-3>mol / L-3.0*10<-3>mol / L and cultivating at the constant temperature; (2) removing highly-abundant proteins according to the steps of a serum albumin scavenger; (3) quantifying an obtained protein sample by use of the Bradford method; (4) performing isoelectric focusing according to the protein loading quantity of 1300 [mu]g / 24 cm of a rubber strip; (5) transferring for polyacrylamide gel electrophoresis after balancing of the rubber strip; (6) after finish of electrophoresis, performing Coomassie brilliant blue staining, decolorization, storage and scanning analysis; (7) performing enzymolysis and mass spectrum identification on differential protein spots.

The invention discloses a method and a special primer set for identifying facticity of to-be-tested variety belonging to CCRI 63. The method provided by the invention comprises the following steps: respectively carrying out PCR (Polymerase Chain Reaction) amplification by use of CIR62, CS62, NAU1070, NAU1102, NAU1186, NAU2026, NAU2173, NAU2277, NAU2343, NAU1196, NAU1085, NAU1255, NAU1369, NAU1233, NAU1269, NAU2272, NAU1187, NAU1375, CS51, NAU1028, NAU2342, NAU868, HAU1300, NAU6094, DPL0238, NAU1362, NAU859, HAU2786, CGR6410 and NAU0478 primers, if a PCR amplification product meets more than 28 standards in the standards of 1)-30), and the to-be-tested cotton is CCRI 63, wherein the genomic DNA of to-be-tested cotton is taken as a template. The method disclosed by the invention can be applied to controlling the quality of cotton seeds, ensuring the quality of commercially available CCRI 63 seeds and fighting against counterfeiting CCRI 63 seeds.

The invention provides an identification method for south radix isatidis in a Chinese herbal compound. The method mainly includes the steps of: first preparing a south radix isatidis reference solution, a test solution, and a negative sample solution free of south radix isatidis, and applying them on one silica gel G thin layer plate in order, then preparing a developing agent of certain proportion to develop the thin layer plate, finally inspecting the thin layer plate and carrying out identification. Specifically, in a test sample chromatogram, fluorescent principal spots in one color are displayed at positions corresponding to those of a reference sample chromatogram, and a negative sample has no display of such spots. With the advantages of simple and fast operation, good separation effect, strong specificity, and wide application, etc., the method provided in the invention is also in favor of perfecting drug quality standards related to south radix isatidis at the same time.

The method of identifying soybean seed authenticity and / or variety purity includes the following steps: mechanically crushing single seed to be detected and adding CTAB buffering liquid; treating in water bath at 65 deg.c for 20-40 min and adding DNA extracting liquid to obtain the genome DNA of each seed; PCR amplification with the extracted genome DNA as template and SSR primer, and performing 6-9 % non-denatured polyacrylamide gel electrophoresis of the PCR amplification product to obtain electropherogram of each seed; and comparing the obtained electropherogram with the standard electropherogram to obtain the soybean seed authenticity and / or variety purity. The method has the features and key technology of SSR marker retained, simplified experiment process, fast identification speed, low cost, accurate result and other advantages.

The invention belongs to the technical field of biology, and discloses an electrophoresis method for removing Rubisco from plant leaves and enriching and separating residual proteins. The method comprises the following steps of: removing Rubisco from watermelon leaves by using 18 percent PEG-4000; leaching residual proteins through a TCA-acetone precipitation method; and performing two-dimensional electrophoresis to separate residual proteins from watermelon leaves. Compared with a holoprotein two-dimensional electrophoretogram, the method has the advantages that: after Rubisco in watermelon leaves is precipitated by adopting the 18 percent PEG, most large and small subunits of Rubisco disappear, the expression levels of most residual proteins are raised, the quantity of proteins obtained by separating is increased by 48.4 percent, and a large quantity of new protein spots (65+ / -15) appear in a region of which the molecular weight is smaller than 25.0kDa. As proved by repeated experiments, the electrophoresis method has the advantages of high repeatability, clear spectra and reliable result.

The invention belongs to the field of photoresist resins, and in particular relates to a method for testing photoresist resin components, comprising the following steps: S1, dissolving the photoresist resin to be tested in a solvent, obtaining hydrogen spectra and Carbon spectrum; S2, analyze the hydrogen spectrum and the carbon spectrum, if the monomer peaks in the spectrum do not overlap each other, then get the molar ratio of each monomer in the photoresist resin according to the peak area, if the monomer peaks in the spectrum overlap with each other Overlap, then transfer to S3; S3, perform thermogravimetric analysis on the photoresist resin to be tested, and calculate the molar ratio of each monomer in the photoresist resin. The beneficial effect of the present invention is: when other test methods such as HNMR test, FTIR test will appear that the characteristic peaks overlap together, and the characteristic peaks cannot be distinguished. At this time, thermal analysis can be used as an accurate analysis method to analyze the photoresist resin components.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

The invention relates to the protein two-dimensional electrophoresis technology of a Mongolian ammopiptanthus, which adopts a two-dimensional electrophoresis technology to prepare the protein of a plant living in extreme environment. The experiment carried out to the Xinjiang ammopiptanthus living in the extreme environment by the technical proposal achieves good effect. At present, repeated experiments certify that: the two-dimensional electrophoresis technology has complete prepared laboratory samples, good repeatability, clear atlas and reliable results, can provide technical examples for the research of plant proteomics threatened by adverse circumstances and is a two-dimensional electrophoresis technology applicable to the proteomic analysis of the Xinjiang ammopiptanthus.

The invention discloses technology for classifying biochemical fingerprint spectrums of Gastrodia elata Blume and identifying quality of the Gastrodia elata Blume and application thereof, and relates to Chinese medicament and natural medicament identification and quality evaluation technology. The invention provides the following key technical parameters for separating and detecting biochemical components of the Gastrodia elata Blume: the optimum mobile phase (acetonitrile solution with gradient concentration), the optimum detection wavelength (219nm) and other basic conditions, and provides the technology for detecting the biochemical fingerprint spectrums of the Gastrodia elata Blume and a formula and a method for calculating the content of an effective component gastrodin in the Gastrodia elata Blume. The technology can be used for detecting the biochemical fingerprint spectrums of the Gastrodia elata Blume, classifying varieties of the Gastrodia elata Blume and identifying and evaluating the quality of the Gastrodia elata Blume, and also can be used for determining regions suitable for growing the Gastrodia elata Blume and identifying the truth of the Gastrodia elata Blume.

The invention provides an analysis method of pulp proteome in a cucumis melo.L. The method comprises the following steps: extracting pulp protein in the cucumis melo.L, firstly performing isoelectric focusing electrophoresis, and then performing SDS-PAGE gel vertical electrophoresis. The invention provides an economic, simple, easily-implemented and fast method suitable for the extraction and dimensional electrophoresis of total protein in the pulp of the cucumis melo.L. The protein is extracted through a Tris / phenol extraction-methanol / ammonium acetate precipitation method, the protein in the pulp of the cucumis melo.L can be effectively extracted, and the influences from mass sugar and other organic matters are reduced; at present, the repeated experimental results show that the experimental sample is comprehensive in preparation, the 2-DE electrophoresis operation is simple, the graph is clear, the repeatability is good, the reagent is low in toxicity and the result is reliable, a technical example is provided for the research of proteomes of the cucurbitaceae horticultural plants, and the method is suitable for analysis of the pulp proteome in the cucumis melo.L by the dimensional electrophoresis technology.

The invention provides an extraction method of bergamot pear proteins. The extraction method comprises the following steps: grinding bergamot pear pulp in a liquid nitrogen atmosphere to obtain a grinded object; mixing the grinded object with polyvinylpolypyrrolidone to obtain a mixture containing the polyvinylpolypyrrolidone; mixing the mixture containing the polyvinylpolypyrrolidone with an extraction liquid and Tris saturated phenol, performing vibration after mixing, putting the vibrated material on ice to insulate, and centrifugalizing the insulated slurry to obtain a first phenol phase;mixing the first phenol phase with the extraction liquid, vibrating the mixture, putting the vibrated slurry on ice to insulate and centrifugalizing the insulated slurry to obtain a second phenol phase; and mixing the obtained second phenol phase with an ammonium acetate methanol liquid, vibrating the mixture, putting the vibrated slurry on ice to insulate, and leaving the insulated slurry overnight at -10-30 DEG C to obtain the bergamot pear proteins. By adopting the extraction method provided by the invention, the quantity of differentiating protein points can be increased.