A method for identifying whether the species to be tested is China Cotton Research Institute 63 and its special primer set

Abstract

The invention discloses a method and a special primer set for identifying facticity of to-be-tested variety belonging to CCRI 63. The method provided by the invention comprises the following steps: respectively carrying out PCR (Polymerase Chain Reaction) amplification by use of CIR62, CS62, NAU1070, NAU1102, NAU1186, NAU2026, NAU2173, NAU2277, NAU2343, NAU1196, NAU1085, NAU1255, NAU1369, NAU1233, NAU1269, NAU2272, NAU1187, NAU1375, CS51, NAU1028, NAU2342, NAU868, HAU1300, NAU6094, DPL0238, NAU1362, NAU859, HAU2786, CGR6410 and NAU0478 primers, if a PCR amplification product meets more than 28 standards in the standards of 1)-30), and the to-be-tested cotton is CCRI 63, wherein the genomic DNA of to-be-tested cotton is taken as a template. The method disclosed by the invention can be applied to controlling the quality of cotton seeds, ensuring the quality of commercially available CCRI 63 seeds and fighting against counterfeiting CCRI 63 seeds.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying whether a variety to be tested is Zhongmian Institute 63 and a special primer set thereof. Background technique [0002] Cotton has obvious heterosis. Hybrid cotton varieties have been used for many years, and the effect of increasing yield is obvious, and they have been popularized and applied in a large area. At present, the hybrid seed production of cotton is still dominated by artificial seed production, and the sterile line seed production has not been promoted. With the continuous rise of labor costs, the cost of hybrid seed production is getting higher and higher, and the quality of seeds is getting worse. The hybrid cotton seeds sold are seriously fake, with F 2 Replace F 1 , with regular cotton instead of F 1 , There are phenomena such as female parent doping, so that the hybrids cannot achieve the due effect of increasing production i...

AI Technical Summary

Problems solved by technology

At present, the hybrid seed production of cotton is still dominated by artificial seed production, and the sterile line seed production has not been promoted. With the continuous rise of labor costs, the cost of hybrid seed production is getting higher and higher, and the quality of seeds is getting worse. The hybrid cotton seeds sold are seriously fake, with F 2 Replace F 1 , with regular cotton instead of F 1 , there are phenomena such as female parent doping, so that the hybrids cannot achieve the due effect of increasing production in the promotion, and affect the promotion effect of hybrid cotton

Conventional field identification takes a long time, which will affect the sales of seeds in the current year, and the identification effect is not very satisfactory due to the impact of the environment during the Jiadai identification in Hainan

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