A kind of extraction method of fragrant pear protein

Abstract

The invention provides an extraction method of bergamot pear proteins. The extraction method comprises the following steps: grinding bergamot pear pulp in a liquid nitrogen atmosphere to obtain a grinded object; mixing the grinded object with polyvinylpolypyrrolidone to obtain a mixture containing the polyvinylpolypyrrolidone; mixing the mixture containing the polyvinylpolypyrrolidone with an extraction liquid and Tris saturated phenol, performing vibration after mixing, putting the vibrated material on ice to insulate, and centrifugalizing the insulated slurry to obtain a first phenol phase;mixing the first phenol phase with the extraction liquid, vibrating the mixture, putting the vibrated slurry on ice to insulate and centrifugalizing the insulated slurry to obtain a second phenol phase; and mixing the obtained second phenol phase with an ammonium acetate methanol liquid, vibrating the mixture, putting the vibrated slurry on ice to insulate, and leaving the insulated slurry overnight at -10-30 DEG C to obtain the bergamot pear proteins. By adopting the extraction method provided by the invention, the quantity of differentiating protein points can be increased.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting fragrant pear protein. Background technique [0002] Korla Fragrant Pear belongs to Xinjiang pear species. It is originally produced in Bayingoleng Mongolia Autonomous Prefecture, Aksu and other places in southern Xinjiang. It has a cultivation history of 1,300 years and is an ancient local excellent variety. Korla fragrant pear has thin skin, crisp texture, white flesh, tender flesh, juicy and sweet taste, slightly sour near the core, and strong fragrance. The planting area in Korla City, Bazhou is 24,000 mu, and the annual output is more than 1,000 tons. , exported to the United States, Southeast Asia, Hong Kong, Macao and other countries and regions. [0003] However, the occurrence of pear blackhead has seriously affected the fruit quality and commodity value of pears. Pear blackhead is a latent infectious disease. According to the ...

AI Technical Summary

Problems solved by technology

At present, the TCA-acetone method is often used to extract the protein in fragrant pears, but the protein spots in the two-dimensional electrophoresis map obtained from the protein are blurred, the resolution is low, and the problems of horizontal and vertical stripes and tailing are serious. After statistical analysis by PDQuest 8.0.1 software, in the same Under the parameter setting conditions, the TCA / acetone precipitation method can distinguish 126 protein spots on the gel surface, and the obtained protein spots are very few

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