The invention discloses a biologically fermented health care wine and a preparation method thereof. The biologically fermented health care wine is prepared from the following raw materials in parts by weight: 20-60 parts of purslane, 40-80 parts of plantain seeds, 30-60 parts of polygonatum sibiricum, 30-60 parts of acanthopanax, 2-6 parts of old cypresses, 1-6 parts of wild ginseng, 10-20 parts of truffle, 10-20 parts of horseradish tree seeds, 60-100 parts of Chinese wolfberries, 30-50 parts of hawthorn fruits, 30-50 parts of cassia seeds and 1000-1300 parts of rice wine. The biologically fermented health care wine is prepared by 12 steps of rice soaking, rice steaming, cooling, rice steaming and storing of processed traditional Chinese medicinal materials in a jar, adding of yeast, mixing, fermenting, adding of the wine, storing in the jar, squeezing, ageing in the jar, and obtaining of the wine. The preparation method for brewing the biologically fermented health-care wine has the advantages that the preparation conditions are mild, the operation is simple, the success rate is high, the production time is relatively short in comparison with routine wines, the nutrient is rich, and the body can be nourished by drinking the health-care wine frequently; and meanwhile, the alcohol content is relatively low, so that the health-care wine can be effectively suitable for people of different ages.