The invention relates to a rubber composition. The composition comprises a main body rubber, a vulcanizing agent, a vulcanization accelerator, an activator and a reinforcing agent. The composition also comprises liquid isoprene rubber. The main body rubber comprises natural rubber, butadiene rubber and solution polymerized styrene-butadiene rubber. The invention also discloses tyre tread rubber and a preparation method thereof. The tyre tread rubber provided by the invention is low in processing energy consumption, good in surface quality of extruding and calendaring rubber materials, good in physical and mechanical properties and ageing resistant property, and relatively small in compression set and heat generation, and can improve durability of the tyre and prolong the service life of the tyre. The tyre tread rubber provided by the invention has good wet skid resistance and low rolling resistance, can guarantee security of the tyre in a driving process and is benefit for energy conservation of the tyre.