The invention relates to a disposable puncture device, which belongs to a surgical instrument matched with an external laparoscope. The invention is suitable for being used for establishing abdominal cavity surgical operation passages in the laparoscope examination and surgical process. The disposable puncture device comprises a puncture sleeve pipe, an air barrier sealing ring, a puncture devicebody, an air injection valve, a self regulating type sealing cap, an air barrier sealing sheet, a locking fixed cover, an elastic sealing cap, an upper fixed ring, a lower fixed ring, a plane sheet sealing ring, a press ring, a puncture positioning guide cover, a puncture awl and a puncture positioning cover, wherein the air barrier sealing ring is arranged on an air barrier sealing ring installing step of the puncture sleeve pipe, the puncture sleeve pipe is arranged in a puncture sleeve pipe installing hole of the lower part of the puncture device body, the air injection valve is arranged in an air injection valve installing hole at one side of the puncture device body, the air barrier sealing sheet is arranged in an air barrier sealing sheet installing clamp ring of the self regulatingtype sealing cap, the self regulating type sealing cap is arranged on a self regulating type sealing cap positioning ring at the upper part of the puncture device body, and the puncture awl is penetrated and inserted in the puncture sleeve pipe through the puncture positioning guide cover, a plane sealing sheet, the elastic sealing ring and the air barrier sealing sheet.