The invention discloses a combined chemiluminescent detection kit and a detection method. The combined chemiluminescent detection kit comprises a specific conjugate of a to-be-detected substance, a
microparticle, a
solid-phase film, a chemiluminescent
reagent, a centrifugal device and a luminescent
detector, wherein the chemiluminescent
reagent comprises at least one selected from a group consisting of a chemiluminescent
enzyme, a chemiluminescent substance, a chemiluminescent
enzyme reaction substrate and a starting luminescent
reagent; the
microparticle is a particle capable of specifically binding to a
protein and / or the chemiluminescent reagent in a
direct binding and / or chemically crosslinking way and maintaining stable; the
solid-phase membrane is a membranous substance having the characteristic of non-specific binding to a
protein; the centrifugal device is a structure capable of centrifugally driving chromatographic flowing of a
liquid phase on the
solid-phase membrane; and the specific conjugate of the to-be-detected substance is at least one selected from a group consisting of
antigen,
antibody,
avidin,
biotin and analogue thereof with specific
binding ability. The combined chemiluminescent detection kit and the detection method in the invention have the characteristics of high sensitivity and short detection time, and have good application prospects.