A multi-channel evanescent wave all-fiber biosensor

Abstract

The invention relates to a multi-channel evanescent wave all-optical fiber biosensor. The biosensor is characterized in that it comprises a flow sample injection system, an optical system and a control circuit and signal processing system; laser emitted by a semiconductor laser unit is collimated and then divided into a plurality of light beams, each light beam is emitted by a corresponding all-optical fiber system to a corresponding sample pool, each optical fiber probe produces fluorescence after excitation, the generated fluorescence signals are collected and filtered by all-optical fiber systems and are then emitted to a photoelectric detector group, the photoelectric detector group converts the collected fluorescence signals of all the optical fiber probes' surfaces into current signals, multi-channel current signals are processed by a micro-signal simulation preamplifier and a multi-channel lock-in amplifier and then sent to a computer, thus realizing simultaneous quantitative analysis of a sample's multiple indexes; and the computer is also connected to a cooperative control module through a parallel processing module, the parallel processing module is also connected to the semiconductor laser unit through a pulse signal generator, and the cooperative control module is in parallel connection with a peristaltic pump and a ten-position valve.

Description

technical field [0001] The invention relates to a multi-channel evanescent wave all-fiber biosensor based on the principle of total internal reflection evanescent wave and the principle of fluorescence analysis. Background technique [0002] As an indispensable step in environmental protection work, environmental monitoring can scientifically measure various factors that affect environmental quality. At present, there is still a lack of economical and practical analytical instruments and methods for the trace toxic pollutants in the water environment. At present, most of the traditional laboratory instrument analysis methods (such as gas chromatography, liquid chromatography, etc.) are used to analyze toxic risk substances, and these instruments Usually only a certain type of substance can be detected, so if it is necessary to detect organic pollutants, heavy metals and biotoxins, it is necessary to be equipped with atomic absorption instrument, HPLC, UPLC, ICP-MS ( Inducti...

AI Technical Summary

Problems solved by technology

By measuring these changes, the information on the changes of the biological sensitive element or optical fiber can be obtained, that is, the information on the interaction between the biological sensitive element and the analyte. When the fluorescent molecule is excited, the fluorescence intensity has a quantitative relationship with the concentration of the biological substance, so that it can be Realize the quantitative determination of biological substances. The evanescent wave all-fiber biosensor based on this principle has the advantages of high sensitivity, strong biological specificity, simple operation, and fast measurement speed. However, the existing single-index optical fiber biosensor can only detect An indicator, there are certain limitations in the scope of use

Images