The invention discloses a tissue culture method for lindera glauca. With the young, tender axillary buds of lindera glauca as explants, a rapid in vitro propagation method based on tissue culture, which includes obtainment of aseptic materials, induced differentiation and multiplication of adventitious buds, rooting of the buds, transplantation and the like, is established. The tissue culture method improves culture medium, adds glutamine regulator, greatly increases the differentiation rate, leaves out the aseptic induced rooting step in tissue culture, adopts open operation, and utilizes NAA to induce rooting, so that complete plants are grown; and the method overcomes the defects of long conventional lindera glauca-breeding period, low propagation coefficient and the like, enhances thestability of the trait of lindera glauca, can realize the factory mass production of seedlings, and can effectively solve the problem on how to supply high-quality lindera glauca seedlings.