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Chinese herbaceous peony extract and preparation method and application thereof

A technology of extract and peony, applied in the field of peony extract and its preparation, can solve the problems of low extraction rate, difficult purification, complicated process, etc., achieve high extraction rate, keep bright and elastic, and take a short time

Pending Publication Date: 2021-01-26
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the traditional methods for preparing pentagalloyl glucose from Paeoniae officinalis face the problem of difficult purification, which requires multiple enrichment of macroporous adsorption resins to achieve purification, the process is relatively complicated, and the extraction rate is low

Method used

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  • Chinese herbaceous peony extract and preparation method and application thereof
  • Chinese herbaceous peony extract and preparation method and application thereof
  • Chinese herbaceous peony extract and preparation method and application thereof

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preparation example Construction

[0028] The invention provides a preparation method of Paeoniae officinalis extract, comprising the steps of:

[0029] Taking peony root and pulverizing it to prepare peony root powder; the particle size of the peony root powder is not more than 850 μm;

[0030] extracting the peony root powder with a methanol aqueous solution with a volume concentration of 45-55%, and concentrating the obtained extract to prepare the first extract; the extraction method is heating to 30-75° C. for reflux extraction for 30-150 minutes;

[0031] adding ethyl acetate to the first extract for extraction, collecting the ethyl acetate phase and concentrating to dryness to prepare a second extract;

[0032] After the second extract is reconstituted, methanol is used as the eluent for gel elution, and the eluate containing the marker is detected and collected; the marker is pentagalloyl glucose.

[0033] Understandably, "gel elution" refers to elution with a gel column. "Concentrating the resulting ...

Embodiment 1

[0049] This embodiment provides a preparation method of Paeoniae officinalis extract, the steps are as follows:

[0050] (1) get Radix Paeoniae Alba, pulverize, cross 200 mesh sieves, obtain Radix Paeoniae Alba powder;

[0051](2) Take 5 g of Paeoniae officinalis root powder obtained in step (1), add 250 mL of methanol aqueous solution (volume concentration is 50%), and condense and reflux for extraction at a temperature of 70 ° C for 60 min; the obtained extract is mixed and cooled, 4500 r / min Centrifuge for 20 minutes, and filter under reduced pressure with filter paper with a pore size of 15 μm; the resulting filtrate is concentrated and evaporated to methanol, and the remaining liquid is extracted with ethyl acetate, and the ethyl acetate layer is collected, and the extraction is repeated until the ethyl acetate layer is colorless; the obtained ethyl acetate phase is concentrated to dryness to obtain a residue;

[0052] (3) Redissolve the residue of step (2) with methanol...

Embodiment 2

[0055] This embodiment provides a method for preparing a peony extract, the steps of which are the same as those in Embodiment 1, the main difference being that in step (2), the volume concentration of methanol in the methanol aqueous solution is different, which is 45%.

[0056] Specific steps are as follows:

[0057] (1) get Radix Paeoniae Alba, pulverize, cross 200 mesh sieves, obtain Radix Paeoniae Alba powder;

[0058] (2) Take 5 g of Paeoniae officinalis root powder obtained in step (1), add 250 mL of methanol aqueous solution (volume concentration is 45%), and condense and reflux for extraction at a temperature of 70 ° C for 60 min; the obtained extract is mixed, cooled, and 4500 r / min Centrifuge for 20 minutes, and filter under reduced pressure with filter paper with a pore size of 15 μm; the resulting filtrate is concentrated and evaporated to methanol, and the remaining liquid is extracted with ethyl acetate, and the ethyl acetate layer is collected, and the extracti...

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Abstract

The invention relates to a Chinese herbaceous peony extract and a preparation method and application thereof. The preparation method of the Chinese herbaceous peony extract comprises the following steps that Chinese herbaceous peony roots are taken and extracted with a methanol aqueous solution, and the obtained extracting solution is concentrated to prepare a first extract; ethyl acetate is addedinto the first extract for extraction, an ethyl acetate phase is collected and concentrated to be dry, and a second extract is prepared; re-dissolving is carried out on the second extract, then gel elution is carried out, and eluent containing a marker is detected and collected; and the marker is pentagalloyl glucose. The Chinese herbaceous peony extract prepared by the preparation method is highin content of pentagalloyl glucose, high in extraction rate and simple in process.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a peony extract and its preparation method and application. Background technique [0002] Paeonia lactiflora (scientific name: Paeonia lactiflora Pall.), also known as parting grass, prime minister of flowers, belongs to Ranunculaceae, and is a perennial herb of the family Ranunculaceae Paeoniae. Radix Paeoniae Alba has comparatively extensive edible value and medical value, according to analysis, Radix Paeoniae Alba contains paeoniflorin and benzoic acid, and purposes are different because of species, and the traditional Chinese medical science thinks that it has antispasmodic, analgesic, stimulates the menstrual function, and is good for women's abdominal pain, stomach It is effective for convulsions, dizziness, gout, diuresis and other diseases. [0003] Antioxidant is the abbreviation of antioxidant free radicals. The human body continuously generates free radicals ...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/08
CPCA61K8/9789A61Q19/08A61K2800/805
Inventor 裴运林聂艳峰张伟强郭朝万胡露蒲艳
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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