Tissue culture method for anemone

A technique of tissue culture and lotus, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of small number of introduced anemone species and limited seedlings, so as to increase the speed of reproduction, improve the stability, and improve the growth rate of seedlings. The effect of uniformity

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of its bright color, anemone is widely cultivated in gardens, and it is a popular border flower that blooms in autumn. However, as a new species introduced from abroad, the number of introduced anemones is relatively small, and the supply of seedlings is limited to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+NAA0.1mg / L bud induction medium;

[0030] (2) Differentiation and proliferation of buds

[0031] Two weeks after the segments were inoculated on the bud induction medium, the axillary buds began to expand, and yellow-green protrusions appeared. After 3 weeks, bud meristems could be seen. After culturing for another month, the adventitious buds could grow to 2cm, and the adventitious buds were cut off. Transferred to the proliferation medium comprising t...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and then soak them in mercury with a volume concentration of 1‰ for 15 minutes. Rinse with sterile water for 5 times, use sterile filter paper to blot the water on the surface, then cut into 1cm-long segments with axillary buds, and inoculate the segments with MS+6-BA3.0mg / L+NAA0.3mg / On the shoot induction medium of L;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments were inoculated on the bud induction medium for 3 weeks, the axillary buds began to expand, and yellow-green protrusions appeared. After 4 weeks, bud meristems could be seen. After one and a half months of culture, the adventitious buds could grow to 3cm, and the adventitious buds were cut off. Transfer to the proliferation me...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ for 30 minutes. Rinse with sterile water for 6 times, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments with axillary buds, and inoculate the segments with MS+6-BA5.0mg / L+NAA0.5mg / On the shoot induction medium of L;

[0054] (2) Differentiation and proliferation of buds

[0055]After the segments were inoculated on the bud induction medium for 4 weeks, the axillary buds began to expand, and yellow-green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of culture, the adventitious buds could grow to 2cm, and the adventitious buds were cut off. Transfer to the proliferation medium that comp...

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PUM

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Abstract

The invention relates to a tissue culture method for anemone, which comprises the following steps of: acquiring a sterile material, differentiating and proliferating buds, culturing adventitious buds and strong seedlings, performing root culture, hardening and transplanting seedlings, and the like. Compared with the prior art, the method has the advantages of greatly improving the reproduction speed of the anemone and the uniformity of the seedlings, improving the stability of characters of the anemone, and realizing industrial mass production of seedling culture.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to an anemone tissue culture method. Background technique [0002] Anemone is a plant of Ranunculaceae and the genus Anemone, widely distributed all over the world, most commonly found in woodlands and meadows in the northern temperate zone, and is a perennial herb. The basal leaves of anemone are 4-8 pieces, the petiole is 6-30cm long, sparsely villous. The leaves are round and reniform, 3-lobed, with 2-5 flowers, 3.5-5cm in diameter, pink. The flowering period of anemone is autumn, and there are long hairs on the achene. Because of its bright color, anemone is widely cultivated in gardens, and it is a popular border flower that blooms in autumn. However, as a new species introduced from abroad, the number of anemone introduced is relatively small, and the supply of seedlings is limited. Contents of the invention [0003] The object of the present invention is exactly to provid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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