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Tissue culturing method of luosifu crocosmia crocosmiflora

A technique of tissue culture and Mars, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of slow branching, small number of introduced species, limited supply of seedlings, etc., to improve the speed of reproduction and the uniformity of seedlings Effect

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety introduced from abroad, the number of Roosevelt Martian flower introductions is small, the division is slow, and the supply of seedlings is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] In the spring, dig out the small bulbs next to the big bulbs of Roosevelt Martian flower, remove the wrapped stems, rinse them with tap water for 1 hour, put them on the ultra-clean workbench, and soak them in ethanol with a mass concentration of 70% for 10 seconds, with a volume concentration of 0.5 ‰ mercury soaked for 10 min, and then rinsed with sterile water for 4 times, using sterile filter paper to blot the water on the surface of the small bulb, cut the bulb into 0.5 cm segments with axillary buds, and then inoculated it in the medium containing MS+6-BA0 .1mg / L+NAA0.1mg / L of bulblet induction medium;

[0030] (2) Differentiation and proliferation of buds

[0031] Inoculated on the bulblet induction medium, the base of the segment began to expand after 1 week, and yellow-green protrusions appeared. After 3 weeks, bud meristems were visible. After culturing for another month, 0.1cm bulblets grew out. The bulbs were cut of...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] In the spring, dig out the small bulbs next to the big bulbs of Roosevelt Martian flower, remove the wrapped stems, rinse them with tap water for 2 hours, put them on the ultra-clean workbench, and soak them in 75% ethanol for 30 seconds, with a volume concentration of 1 ‰ mercury soaked for 15 minutes, and then rinsed with sterile water for 5 times. After using sterile filter paper to dry the water on the surface of the small bulbs, the bulbs were cut into 1 cm segments with axillary buds, and then inoculated in MS+6-BA5. 0mg / L+NAA0.5mg / L bulb induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] Inoculated on the bulblet induction medium, the base of the segment began to expand after 2 weeks, and yellow-green protrusions appeared, and the bud meristem was visible after 3 weeks, and after culturing for 1 month, 0.3cm bulblets grew out, and the bulblets of 0.3 cm grew. The corm was cut and moved into...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] In the spring, dig out the small corms next to the big corms of Roosevelt Martian flower, remove the wrapped stems, wash them with tap water for 3 hours, and then put them on the ultra-clean workbench, and soak them in ethanol with a mass concentration of 75% for 50 seconds, with a volume concentration of 2 ‰ mercury soaked for 30min, and then rinsed with sterile water for 6 times, using sterile filter paper to blot the water on the surface of the small bulb, cut the bulb into 2cm segments with axillary buds, and then inoculated in the cells including MS+6-BA5. 0mg / L+NAA0.5mg / L bulb induction medium;

[0054] (2) Differentiation and proliferation of buds

[0055]After being inoculated on the bulblet induction medium, the base of the segment began to expand after 3 weeks, and yellow-green protrusions appeared. After 4 weeks, bud meristems were visible. After 2 months of cultivation, 0.2cm bulblets grew out. The corm was cut and ...

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PUM

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Abstract

The invention relates to a tissue cultivating method of luosifu crocosmia crocosmiflora, comprising the steps of acquisition of sterile materials, differentiation and proliferation of buds, cultivation of strong-seedling of adventitious bud, rooting cultivation, hardening seedling, transplanting and the like. Compared with the prior art, the invention greatly improves the reproduction speed of luosifu crocosmia crocosmiflora and the uniformity of seedlings, and can realize industrialized large-scale production of seedlings.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to the tissue culture method of Roosevelt Martian flower. Background technique [0002] Roosevelt Martian flower is a realgar orchid plant of the family Iridaceae, native to southern Africa. It is a perennial herb with bulbs and stolons. The bulbs are oblate and have a brown fibrous membrane outside. The stem above the ground is about 50cm high, often branched, the leaves are linear and sword-shaped, the flowers are orange-red, funnel-shaped, and arranged in multiple bouquets in compound panicles, drawn from the green leaves, scattered in height, dense and dense , the perianth tube is slender and slightly curved, and the lobes spread out. The flowering season of Martian flower is midsummer, and the flowers bloom continuously. It is a good material for arranging flower borders and flower beds. However, as a new variety introduced from abroad, the number of introduced Roosevelt Marti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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