Tissue culture method of floral leaf fatshedera lizei
A technology of tissue culture and mosaic, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of small quantity, changeability, and limited supply of seedlings, so as to improve the speed of reproduction and the uniformity of seedlings, The effect of reducing variability
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Embodiment 1
[0028] (1) Obtaining sterile materials
[0029] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and mercury with a volume concentration of 0.5‰ for 10 minutes, and then use sterile Rinse with water for 4 times, dry the water on the surface with sterile filter paper, then cut into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+NAA0.1mg / L on axillary bud induction medium;
[0030] (2) Differentiation and proliferation of buds
[0031] After the segments were inoculated on the axillary bud induction medium for 1 week, the axillary buds began to expand and green protrusions appeared. After 2 weeks, bud meristems could be seen. After culturing for 1 month, the small adventitious buds could grow to 3 cm. Cut the adventitious buds Under transfer into the proliferation m...
Embodiment 2
[0040] (1) Obtaining sterile materials
[0041] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and soak them in mercury with a volume concentration of 1‰ for 15 minutes, and then use sterile Rinse with water for 5 times, use sterile filter paper to blot the water on the surface, then cut into 1cm-long segments with axillary buds, and inoculate the segments with axillary buds containing MS+6-BA2.0mg / L+NAA0.2mg / L on the induction medium;
[0042] (2) Differentiation and proliferation of buds
[0043] After the segments were inoculated on the axillary bud induction medium for 2 weeks, the axillary buds began to expand and green protrusions appeared. After 3 weeks, bud meristems could be seen. After one and a half months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds ...
Embodiment 3
[0052] (1) Obtaining sterile materials
[0053] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ for 30 minutes. Rinse with water for 6 times, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments with axillary buds, and inoculate the segments with axillary buds including MS+6-BA3.0mg / L+NAA0.3mg / L on the induction medium;
[0054] (2) Differentiation and proliferation of buds
[0055] After the segments were inoculated on the axillary bud induction medium for 3 weeks, the axillary buds began to expand and green protrusions appeared. After 4 weeks, bud meristems could be seen. After 2 months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under transfer into the prolife...
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