The invention discloses a special culture medium for quickly inducing
bulb of
bletilla striata. The special culture medium consists of a seed of
bletilla striata germinating culture medium and a
bulb high-efficiency inducing and
seedling sounding culture medium, wherein the seed of
bletilla striata germinating culture medium is prepared from the following components according to a formula: 1 / 2MS, 0.1mg / L of NAA (
naphthylacetic acid), 0.1mg / L of GA3, 6.5 to 7.0g / L of
agar, and 20g / L of white
sugar, and the pH (potential of
hydrogen) value is 5.8 to 6.0; the
bulb high-efficiency inducing and
seedling sounding culture medium is prepared from the following components according to a formula: 1 / 2MS, 0.5 to 0.6mg / L of NAA, 0.6 to 0.8mg / L of 6-BA, 0.4 to 0.5mg / L of PP333, 30 to 45g / L of white
sugar, 25 to 40g / L of mashed banana, and 0.1g / L of
activated carbon, and the pH (potential of
hydrogen) value is 5.8 to 6.0. The invention also discloses a
tissue culture method of the special culture medium. The
tissue culture method has the advantages that the production steps of the
tissue culture seedling of the bulb of
bletilla striata are simplified, the inducing of
pseudobulb, quick growth of
pseudobulb and rooting and seedling sounding culture are completed at one time, the period from seeds to
transplanting is 90 days, the culture time is shortened, the differentiation rate and quick growth of the
pseudobulb are promoted, and the
transplanting success rate of the tissue culture seedling is increased.