Method for promoting growth and development of plant seedlings by soaking seeds with dark-color septal endophytic fungi

A dark-colored compartmentalized endogenous, plant growth-promoting technique for application, seed and rhizome treatment, agriculture, etc.

Active Publication Date: 2020-07-07
CHINA UNIV OF MINING & TECH (BEIJING) +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seed soaking is the most commonly used method to promote germination and rooting. There are physical and chemical methods for soaking seeds to accelerate germination, but there is no report on the use of DSE microbial bacterial liquid to accelerate germination

Method used

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  • Method for promoting growth and development of plant seedlings by soaking seeds with dark-color septal endophytic fungi
  • Method for promoting growth and development of plant seedlings by soaking seeds with dark-color septal endophytic fungi
  • Method for promoting growth and development of plant seedlings by soaking seeds with dark-color septal endophytic fungi

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, acquisition and preservation of bacterial strains

[0056] The three bacterial strains (needle A2-7, needle A1-3, and No. 001 bacteria) were all strains isolated and purified by the inventor from the root system of Stipa sativa in Xilinhot City, Xilinhot City, Xilin Gol League, Inner Mongolia Autonomous Region.

[0057] The ITS sequence of the detection pin A2-7, as shown in sequence 1 in the sequence listing, was identified as dark septal endophyte (DSE) (Darksidea sp.). Needle A2-7 was deposited in the General Microorganism Center of China Committee for Microorganism Culture Collection on November 19, 2019, with the preservation number CGMCC No.18811. The dark color has the morphological characteristics of septal endophytic fungi: black or dark brown, the hyphae are dark in the root system, and there are obvious transverse septa. The morphology photos of needles A2-7 in the root system are shown in figure 1 .

[0058] The ITS sequence of detection pi...

Embodiment 2

[0060] Embodiment 2, the impact of bacterial liquid on the growth of corn

[0061] The tested strains were: needle A2-7 and needle A1-3 and dark septate endophyte 001.

[0062] The corn variety is Zhongnuo No. 1 (certification number: 0103006-2000, Beijing Zhongnongzuo Technology Development Co., Ltd.). Zhongnuo No. 1 is also known as Zhongnuo No. 1.

[0063] 1. Preparation and dilution of needle A2-7 bacterial solution

[0064] 1. Take the bacteria cake with a diameter of 6mm from the needle A2-7, inoculate it into 100ml liquid MMN medium, culture it with shaking at 25°C, and obtain the bacteria solution. Cultivation process: 180r / min shaking culture for 1 day, then 160r / min shaking culture for 7 days.

[0065] 2. Detection of bacterial concentration

[0066] After completing step 1, sample 1 mL of the bacterial liquid, filter through filter paper to collect the mycelium, then dry it at 105°C to constant weight and weigh it, which is the dry weight of the mycelium. Calcu...

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Abstract

The invention discloses a method for promoting growth and development of plant seedlings by soaking seeds with dark-color septal endophytic fungi. The invention provides a plant seed pretreatment method. The method comprises the following steps: soaking plant seeds with a bacterial solution of dark-color septal endophytic fungi (Darksidea sp) needle A2-7, wherein the preservation number of the dark-color septal endophytic fungi needle A2-7 is CGMCC No. 18811; the bacterial solution of the dark-color septal endophytic fungi needle A2-7 can be specifically a 20% needle A2-7 bacterial solution; and the soaking time can be specifically 12 hours. By soaking corn seeds with the bacterial solution provided by the invention, the growth of corn seedlings can be obviously promoted while the rapid germination of seeds is promoted. The method disclosed by the invention has great application and popularization values for plant planting, especially crop planting.

Description

technical field [0001] The invention relates to a method for promoting the growth and development of plant seedlings by soaking seeds with dark-colored compartmentalized endophytic fungi. Background technique [0002] Dark septate endophytes (Dark Septate Endophytes, DSE) have similar biological functions to mycorrhizal fungi. Its ecological distribution is wide, and its colonization in plants in different habitats shows that they have little or no host specificity, and can establish a mutually beneficial and mutually regulated physiological whole with plants without causing plant diseases, and each has its own morphological characteristics. [0003] Studies have shown that dark compartmentalized endophytic fungi play a more important role in stress environments, expanding the absorption area of ​​plant roots through the growth of mycelia, improving the scope of root absorption and the ability to absorb nutrients. During the process of plant growth play an important role in...

Claims

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Application Information

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IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 毕银丽薛子可全文智
Owner CHINA UNIV OF MINING & TECH (BEIJING)
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