The invention discloses fish oytocin oral granules and preparation and using methods thereof, and belongs to the technical field of fish culture. Aiming at the problems of difficult hormone delivery, complex operation, high cost, low ecbolic efficiency, high parent fish death rate and the like in artificial propagation of certain small fishes, the invention provides an artificial ecbolic method, which avoids fish stress response death and is high in ecbolic rate, easy, convenient, feasible and low in cost. The method comprises the following steps of: dissolving oytocin into normal saline, spraying the solution to an aquatic granulated feed, preparing the fish oytocin oral granules by wrapping a layer of animal grease containing vitamin E on the surface of the aquatic granulated feed, feeding the fishes with the oytocin oral granules in the propagation season of the fishes, and delivering the external hormone to parent fishes when the fishes actively eat the oral granules to induce gonad maturation, laying and fertilization of the parent fishes. The oral granules and the method are mainly applied to the artificial propagation of certain small fishes.