The invention discloses a preparation method of a
selenium-rich
lactobacillus preparation. The preparation method comprises the following steps: (1), inoculating cryopreserved Lb.plantarum ST-III strain in an MRS
liquid culture medium and carrying out anaerobic culture, and circularly subculturing for 2 times to 3 times to obtain a fugus body culture; (2), weighing 2g of
selenium-rich tea samples,adding
purified water into the 2g of
selenium-rich tea samples to obtain a mixture; soaking the mixture and taking supernant liquid; and filtering the supernant liquid through a aseptic
filtration film to obtain selenium-rich tea extract; (3), preparing a tomato juice-TE culture medium (4) and performing
centrifugation to obtain the fugus body culture, and collecting a fugus body; (5), cleaning the fugus body collected in the step (4) with normal
saline for three times, and resuspending the fugus body in the tomato juice-TE culture medium obtained in the step (3), and uniformly shaking and standing at a temperature of 27 DEG C and performing
fermentation for 48 hours to 96 hours; and (6), centrifuging the obtained
fermentation fugus body, and aseptically washing the fugus body for three times with the normal
saline, and performing centriguation and removing supernant liquid to obtain the selenium-rich
lactobacillus preparation. The preparation method of the selenium-rich lactobacilluspreparation disclosed by the invention has the
advantage that organic selenium is effectively enriched from the selenium-rich tea by adopting a
fermentation means.