Selenium-enriched lactobacillus preparation and preparation method thereof

A technology of lactic acid bacteria and preparations, applied in the directions of biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve problems such as limited guiding significance, and achieve the effect of increasing the content of selenium

Pending Publication Date: 2020-04-03
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the layout of relevant intellectual property rights, the current research on selenium-enriched lactic acid bacteria is only limited to a few strains of Lactobacillus; more importantly, the existing research is the use of lactic acid bacteria in synthetic media for chemical inorganic selenium reagents. (mainly sodium selenate), the relevant results have limited guiding significance for the food industry

Method used

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  • Selenium-enriched lactobacillus preparation and preparation method thereof
  • Selenium-enriched lactobacillus preparation and preparation method thereof

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Embodiment 1

[0031] A preparation method for selenium-enriched lactic acid bacteria preparation, comprising the following steps:

[0032] (1) Activated strains: Inoculate the cryopreserved Lb.plantarum ST-III strains in MRS liquid medium, culture anaerobically at 37°C for 18-22 hours, subculture 2-3 times in a cycle, and obtain bacterial cultures Wherein the composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, 54mg of manganese sulfate and 1000mL of distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15 minutes;

[0033] (2) Preparation of Enshi selenium-enriched tea extract: Weigh 2g of Enshi selenium-enriched tea sample, soak in 100mL pure water at 100°C for 10 minutes, take the supernatant, filter through a 0.44μm sterile filter membrane, and extract Enshi selenium-enriched tea thing;

[0034] (3...

Embodiment 2

[0042] A preparation method for selenium-enriched lactic acid bacteria preparation, comprising the following steps:

[0043] (1) Activated strains: Inoculate the cryopreserved Lb.plantarum ST-III strains in MRS liquid medium, culture anaerobically at 37°C for 18-22 hours, subculture 2-3 times in a cycle, and obtain bacterial cultures Wherein the composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, 54mg of manganese sulfate and 1000mL of distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15 minutes;

[0044] (2) Preparation of Enshi selenium-enriched tea extract: Weigh 2g of Enshi selenium-enriched tea sample, soak in 100mL pure water at 100°C for 10 minutes, take the supernatant, filter through a 0.44μm sterile filter membrane, and extract Enshi selenium-enriched tea thing;

[0045](3)...

Embodiment 3

[0052] A preparation method for selenium-enriched lactic acid bacteria preparation, comprising the following steps:

[0053] (1) Activated strains: Inoculate the cryopreserved Lb.plantarum ST-III strains in MRS liquid medium, culture anaerobically at 37°C for 18-22 hours, subculture 2-3 times in a cycle, and obtain bacterial cultures Wherein the composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, 54mg of manganese sulfate and 1000mL of distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15 minutes;

[0054] (2) Preparation of Enshi selenium-enriched tea extract: Weigh 2g of Enshi selenium-enriched tea sample, soak in 100mL pure water at 100°C for 10 minutes, take the supernatant, filter through a 0.44μm sterile filter membrane, and extract Enshi selenium-enriched tea thing;

[0055] (3...

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Abstract

The invention discloses a preparation method of selenium-enriched lactobacillus preparation. The method includes the following steps: (1) inoculating frozen-stored Lb. platanum ST-III strains in an MRS liquid medium, performing anaerobic culture and subculture to obtain a bacterial culture; (2) weighing 2 g of green tea sample, soaking the green tea sample in purified water for 10 min, taking supernatant, and filtering the supernatant through a sterile filter to obtain green tea extract; (3) preparing an MRS-TE medium: cooling the MRS-Mn medium to room temperature naturally, and then adding the green tea extract; (4) centrifuging the obtained bacterial culture to collect the bacterial cells; (5) washing the collected bacterial cells three times with physiological saline and then resuspending the bacterial cells in the MRS-TE medium, shaking thoroughly, and leaving to stand for fermentation at 37 DEG C; and (6) centrifuging the fermented bacterial cells, washing the fermented bacterialcells with sterile physiological saline three times, and removing the supernatant to obtain the selenium-enriched lactobacillus preparation. The method has an advantage of enriching organic selenium from green tea by fermentation.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria preparations, in particular to a selenium-enriched lactic acid bacteria preparation and a preparation method thereof. Background technique [0002] Selenium is an essential trace mineral element for the human body and an important element for maintaining the normal growth and metabolism of life; it has been jointly confirmed by the Food and Agriculture Organization of the United Nations (Food and Agriculture Organization, FAO) and the World Health Organization (World Health Organization, WHO) as an essential element for the human body An important trace element, which is closely related to the maintenance of normal physiological functions of the body and the occurrence and development of various diseases, and is an essential component of many enzyme activity centers in mammals, such as glutathione peroxidase (glutathione peroxidase, GPx ), deiodinase (iodothyroninedeiodinases, ID), et...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12R1/25
CPCC12N1/20C12N1/38
Inventor 鄢明辉刘振民游春苹扶晓菲
Owner BRIGHT DAIRY & FOOD
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