Selenium-rich lactobacillus preparation and preparation method thereof

A technology of lactic acid bacteria and preparations, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as limited guiding significance, and achieve the effect of increasing the content of selenium

Pending Publication Date: 2020-04-03
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the layout of relevant intellectual property rights, the current research on selenium-enriched lactic acid bacteria is only limited to a few strains of Lactobacillus; more importantly, the existing research is the use of lactic acid bacteria in synthetic media for chemical inorganic selenium reagents. (mainly sodium selenate), the relevant results have limited guiding significance for the food industry

Method used

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  • Selenium-rich lactobacillus preparation and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of selenium-enriched lactic acid bacteria preparation includes the following steps:

[0030] (1) Activated strain: inoculate the cryopreserved Lb.plantarum ST-III strain in MRS liquid medium, culture it anaerobicly at 37℃ for 18-22h, circulate subculture 2-3 times, and obtain bacterial culture The composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, Adjust the pH to 6.5 with 54 mg of manganese sulfate and 1000 mL of distilled water, and sterilize at 121°C for 15 minutes.

[0031] (2) Preparation of green tea extract: Weigh 2g of green tea sample, add 100℃, 100mL purified water to soak for 10 minutes to take the supernatant, filter through a sterile filter membrane to obtain green tea extract;

[0032] (3) Configure tomato juice-TE medium: the preparation process of tomato juice: w...

Embodiment 2

[0040] A preparation method of selenium-enriched lactic acid bacteria preparation includes the following steps:

[0041] (1) Activated strain: inoculate the cryopreserved Lb.plantarum ST-III strain in MRS liquid medium, culture it anaerobicly at 37℃ for 18-22h, circulate subculture 2-3 times, and obtain bacterial culture The composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, Adjust the pH to 6.5 with 54 mg of manganese sulfate and 1000 mL of distilled water, and sterilize at 121°C for 15 minutes.

[0042] (2) Preparation of green tea extract: Weigh 2g of green tea sample, add 100℃, 100mL purified water to soak for 10 minutes to take the supernatant, filter through a sterile filter membrane to obtain green tea extract;

[0043] (3) Configure tomato juice-TE medium: the preparation process of tomato juice: w...

Embodiment 3

[0050] A preparation method of selenium-enriched lactic acid bacteria preparation includes the following steps:

[0051] (1) Activated strain: inoculate the cryopreserved Lb.plantarum ST-III strain in MRS liquid medium, culture it anaerobicly at 37℃ for 18-22h, circulate subculture 2-3 times, and obtain bacterial culture The composition of MRS liquid medium is as follows: 10g peptone, 10g beef extract, 5g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1g Tween 80, 200mg magnesium sulfate, Adjust the pH to 6.5 with 54 mg of manganese sulfate and 1000 mL of distilled water, and sterilize at 121°C for 15 minutes.

[0052] (2) Preparation of Enshi selenium-enriched tea extract: Weigh 2g of Enshi selenium-enriched tea sample, add 100°C, 100mL purified water for 10 minutes to soak the supernatant, filter through a sterile filter membrane to obtain Enshi selenium-enriched tea extract;

[0053] (3) Configure tomato juice-TE med...

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Abstract

The invention discloses a preparation method of a selenium-rich lactobacillus preparation. The preparation method comprises the following steps: (1), inoculating cryopreserved Lb.plantarum ST-III strain in an MRS liquid culture medium and carrying out anaerobic culture, and circularly subculturing for 2 times to 3 times to obtain a fugus body culture; (2), weighing 2g of selenium-rich tea samples,adding purified water into the 2g of selenium-rich tea samples to obtain a mixture; soaking the mixture and taking supernant liquid; and filtering the supernant liquid through a aseptic filtration film to obtain selenium-rich tea extract; (3), preparing a tomato juice-TE culture medium (4) and performing centrifugation to obtain the fugus body culture, and collecting a fugus body; (5), cleaning the fugus body collected in the step (4) with normal saline for three times, and resuspending the fugus body in the tomato juice-TE culture medium obtained in the step (3), and uniformly shaking and standing at a temperature of 27 DEG C and performing fermentation for 48 hours to 96 hours; and (6), centrifuging the obtained fermentation fugus body, and aseptically washing the fugus body for three times with the normal saline, and performing centriguation and removing supernant liquid to obtain the selenium-rich lactobacillus preparation. The preparation method of the selenium-rich lactobacilluspreparation disclosed by the invention has the advantage that organic selenium is effectively enriched from the selenium-rich tea by adopting a fermentation means.

Description

Technical field [0001] The invention relates to the technical field of lactic acid bacteria preparations, in particular to a selenium-enriched lactic acid bacteria preparation and a preparation method thereof. Background technique [0002] Selenium is an essential trace mineral element for the human body and an important element for maintaining normal growth and metabolism of life; it has been jointly recognized by the Food and Agriculture Organization (FAO) of the United Nations and the World Health Organization (WHO) as an essential element for the human body An important trace element, which is closely related to the maintenance of normal physiological functions of the body and the occurrence and development of various diseases. It is an essential component of many enzyme active centers in mammals, such as glutathione peroxidase (GPx). ), deiodinase (iodothyroninedeiodinases, ID), etc., have the physiological functions of protecting the structure and function of cell membranes...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/25
CPCC12N1/20
Inventor 鄢明辉游春苹刘振民扶晓菲
Owner BRIGHT DAIRY & FOOD
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