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Selenium-enriched yeast and selenium-rich optimization method for same

A technology of selenium-enriched yeast and optimization method, applied in the field of bioengineering, can solve the problems of limited selenium enrichment ability, poor selenium tolerance, poor stability, etc., and achieve the effect of low cost, less addition amount and simple operation procedure

Inactive Publication Date: 2018-01-23
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the selenium-enriched yeast strains used are artificially cultivated strains with poor stability and poor tolerance to selenium, and the ability to enrich selenium is limited.

Method used

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  • Selenium-enriched yeast and selenium-rich optimization method for same
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  • Selenium-enriched yeast and selenium-rich optimization method for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Screening of selenium-enriched yeast

[0021] 1) Select the middle and upper-grade selenium-rich soil with selenium content of 0.6~8.2mg / kg in Shihezi City and northern Shawan County of Xinjiang as sampling sites, and randomly set up 5 sampling points, mainly collect 5cm~10cm soil suitable for microbial growth under the grape surface. Get 50 samples.

[0022] 2) Add 99ml of sterile water and 1g of soil sample to a 150ml Erlenmeyer flask, and incubate for 12h with shaking in a shaker at 30°C and 180 revolutions. Pipette the solution in 100ul Erlenmeyer flask and add it to Martin medium (containing 0.5% peptone, 1% glucose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 2% agar, 0.03% red bengal solution, 0.01% chloramphenicol ), cultured in an incubator at 30°C for 4-5 days.

[0023] 3) According to the morphological characteristics of yeast strains and cells, observe the colonies grown on Martin's medium with naked eyes, and pick out the relatively smooth and ...

Embodiment 2

[0026] Identification of selenium-enriched yeast

[0027] 1) Observing the morphological characteristics of yeast by scanning electron microscope, it is found that the cells are oval, with a width of 3.5~5.0μm and a length of 6.0~10μm, multilateral budding, with obvious red pigment. Many kinds of cells are formed due to the formation of capsules. Quality colonies. After culturing on YPD medium for about 3-5 days, the color of the lawn changes from coral red to orange red. The surface can be smooth to wrinkled, shiny and sticky.

[0028] 2) Extract the genome of high-selenium-enriched yeast X20, design primers to amplify its 18SrDNA sequence, send it to a sequencing company for sequencing, and obtain the following 18SrDNA gene sequence:

[0029]

[0030]

[0031] 3) Submit the above sequence to the National Center for Biotechnology Information (NCBI), and use the BLAST tool to compare and analyze. The results show that the submitted gene sequence matches the R. glutinosa gene sequen...

Embodiment 3

[0033] Adding phosphorus to optimize the selenium enrichment effect of the strain

[0034] Adding sodium dihydrogen phosphate to a medium containing 2% glycerol, 1% yeast powder and 2% peptone can induce the expression of high-affinity phosphate-selenium transporter in yeast, and improve the transport capacity and tolerance of yeast to selenium. When adding 0.1, 0.2, 0.3 or 0.4 mM / L hydrogen phosphate disodium salt to the culture medium, add 50~80μg / mL selenium salt at a concentration of 50~80μg / mL after culturing for 12 hours, shaking at 30℃ for 36 hours, and measuring yeast with atomic fluorescence spectrometer The amount of selenium enriched is as high as 4500-5009μg / g.

[0035] The selenium-enriched content of yeast measured by atomic fluorescence spectrometer is 4500-5009μg / g.

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Abstract

The invention discloses selenium-enriched yeast and a selenium-rich optimization method for the same, and belongs to the field of bioengineering. The selenium-enriched yeast is taxonomically named Rhodotorula glutinis and is named X20, and a preservation serial number of the selenium-enriched yeast is CCTCCM2017225. The selenium-enriched yeast and the selenium-rich optimization method have the advantages that phosphate is added to induce yeast high-affinity phosphate-selenium transport protein expression, and accordingly the selenium transport capacity and the tolerance of the selenium-enriched yeast can be improved by the aid of the selenium-rich optimization method; only phosphorus elements need to be added into culture media, and the selenium-rich optimization method includes simple operation programs and is low in additive volume and cost.

Description

Technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a selenium-enriched yeast and a selenium-enriched optimization method thereof. Background technique [0002] Selenium is one of the essential trace elements for humans and animals. It has biological functions such as improving immunity, reducing mutations of carcinogens, removing free radicals in the body, and inhibiting lipid peroxidation. It can maintain thyroid hormone metabolism and cardiovascular system. , Antioxidant defense system and the normal function of the immune system. Studies have found that more than 40 diseases such as Keshan disease, Kashin-Beck disease, cataracts, and diabetes are all related to selenium deficiency. my country is a severely selenium-deficient country, and 72% of the country's land area is deficient in selenium ( <0.6mg / kg), 30% of which are severely selenium deficient. Selenium in my country is mainly distributed in Enshi, Fengcheng, Lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/645
Inventor 张根林娄兴丹党艳艳
Owner SHIHEZI UNIVERSITY
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