Cultivation method of selenium-enriched cordyceps militaris

A cultivation method and technology of Cordyceps militaris, which is applied in the field of Cordyceps militaris cultivation, can solve problems such as low absorption utilization rate, difficult absorption of Cordyceps militaris, adverse effects of inorganic selenium medium utilization of Cordyceps militaris, etc., to achieve induced absorption and accumulation, improve nutrition and Medicinal value, the effect of increasing the selenium content of Cordyceps sinensis

Pending Publication Date: 2022-07-29
河南天蚕生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And selenium fertilizer nutrient solution mostly adopts sodium selenite as the source of selenium element, adopts sodium selenite as the source of selenium element Cordyceps militaris is difficult to absorb, and its absorption utilization rate is low, safety is poor, causes the Cordyceps militaris fruiting body of cultivating The selenium content is extremely small, it is difficult to improve the nutritional and medicinal value of Cordyceps militaris, and too much inorganic selenium residues have a negative impact on the utilization of Cordyceps militaris culture medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the cultivation method of a kind of selenium-enriched Cordyceps militaris of the present invention, comprises the following steps:

[0025] S1. Activation and cultivation of strains: inoculate the strains of Cordyceps militaris in the activation medium for strains, and cultivate at a constant temperature of 21°C, humidity of 60-85%, and shading for 7 days; the activation medium for strains includes the following qualities Percentage of components: wheat 10%, wheat bran 4%, soybean 8%, sucrose 1%, peptone 1.5%, potassium dihydrogen phosphate 0.8%, triamine citrate 1.2%, selenium-enriched yeast powder 3%, the balance for water.

[0026] S2, liquid bacterial culture: select the product with better activity in step S1, inoculate it in the liquid bacterial culture medium, and shake it at a constant speed under the shaking speed of 120-150 times / min to carry out shaker culture 5-7 The liquid culture medium includes the following components by mass percentage: 1...

Embodiment 2

[0031] Example 2: The difference from Example 1 is:

[0032] The strain activation medium includes the following components by mass percentage: wheat 12%, wheat bran 12%, soybean 12%, sucrose 1.5%, peptone 1%, potassium dihydrogen phosphate 1.2%, triamine citrate 0.5%, rich Selenium yeast powder 2%, the balance is water.

[0033] The liquid culture medium includes the following components by mass percentage: soybean powder 18%, sucrose 6%, peptone 2.2%, potassium dihydrogen phosphate 1.6%, vitamin B 12 0.8%, selenium-enriched yeast powder 2.6%, oak leaf extract 1.2%, and the balance is water.

[0034] The fruiting body selenium-enriched medium includes the following components by mass percentage: wheat 12%, wheat bran 8%, sucrose 3.5%, molasses 2%, peptone 3%, potassium dihydrogen phosphate 1.2%, triamine citrate 1%, Selenium-enriched yeast powder 3%, amino acid chelated calcium 1.5%, and the balance is water.

[0035] After testing, the content of selenium in the fruiting ...

Embodiment 3

[0036] Example 3: The difference from Example 1 is:

[0037] The strain activation medium includes the following components by mass percentage: wheat 15%, wheat bran 8%, soybean 22%, sucrose 2%, peptone 2%, potassium dihydrogen phosphate 1%, triamine citrate 1.5%, rich Selenium yeast powder 5.5%, the balance is water.

[0038] The liquid culture medium includes the following components by mass percentage: soybean powder 20%, sucrose 4%, peptone 1.5%, potassium dihydrogen phosphate 2.3%, vitamin B 12 0.6%, selenium-enriched yeast powder 3.3%, oak leaf extract 2.1%, and the balance is water.

[0039] The fruiting body selenium-enriched medium includes the following components by mass percentage: wheat 15%, wheat bran 12%, sucrose 4%, molasses 1.5%, peptone 4%, potassium dihydrogen phosphate 0.8%, triamine citrate 0.5%, Selenium-enriched yeast powder 5%, amino acid chelated calcium 2%, and the balance is water.

[0040] After testing, the content of selenium in the fruiting bodi...

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PUM

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Abstract

The invention discloses a cultivation method of selenium-enriched cordyceps militaris, and belongs to the technical field of cordyceps militaris cultivation methods. Comprising the following steps: strain activation culture: a strain activation culture medium comprises the following components: wheat, wheat bran, soybeans, cane sugar, peptone, monopotassium phosphate, triamine citrate, selenium-enriched yeast powder and water; culturing a liquid strain, wherein a liquid strain culture medium comprises the following components: soybean meal, cane sugar, peptone, monopotassium phosphate, vitamin B12, selenium-enriched yeast powder, an oak leaf extract and water; selenium-enriched culture of sporocarp, wherein a selenium-enriched culture medium of sporocarp comprises the following components in percentage by mass: wheat, wheat bran, cane sugar, molasses, peptone, monopotassium phosphate, triamine citrate, selenium-enriched yeast powder, calcium amino acid chelate and water. Through the synergistic effect of organic selenium in the culture medium formula, absorption and accumulation of selenium elements are induced, and the selenium content of the cordyceps sinensis can be increased while residue harm caused by addition of inorganic selenium is avoided.

Description

technical field [0001] The invention belongs to the technical field of Cordyceps militaris cultivation methods, and in particular relates to a cultivation method of selenium-enriched Cordyceps militaris. Background technique [0002] Cordyceps militaris (Cordyceps militsris) is an insect-borne fungus belonging to the subphylum Ascomycota and Ergotaceae, also known as Cordyceps militaris. Under the circumstance that Cordyceps sinensis cannot be commercialized artificially, the market is extremely scarce, and the price remains high, Cordyceps militaris has become the focus of development in various industries such as fungi, medicine, health food, and biology at home and abroad for many years. [0003] Selenium is an indispensable trace element in the human body, which has the physiological functions of anti-oxidation and anti-aging. At the same time, selenium deficiency will lead to decreased immunity, Keshan disease, Kashin-Beck disease, cardiovascular and cerebrovascular di...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/20A01G18/40
Inventor 赵岩刘雅丽孟凡玉任崇赟武留超王延朴丁伟任红彦
Owner 河南天蚕生物科技股份有限公司
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