41results about How to "Experimental operation is fast" patented technology

The invention relates to the technical field of electric power, in particular to a system and method for testing the electric conductivity of a metal wire. The system comprises a direct-current power supply, a wire clamping system and a test system. The output end of the direct-current power supply is in series connection with the metal wire to be tested through a fixture. The metal wire to be tested is connected to an optical platform through a fixture. A first camera and a second camera are respectively and electrically connected with a computer. A multiple-pass digital multimeter V is connected with the metal wire to be tested and is further electrically connected with the computer. A multiple-pass digital multimeter A is connected with the metal wire to be tested through the direct-current power supply. Due to the adoption of the system and method for testing the electric conductivity of the metal wire, the electric conductivity of a metal wire of any length and section shape can be tested without considering the effect of the temperature on the resistance of the wire to be tested, and experiments are easy and quick to operate. The system and method greatly improve working efficiency, obviously improve testing accuracy and are suitable for all kinds of novel energy-saving metal wires.

The invention discloses a method for measuring residues of three phenoxy carboxylic acid pesticides in tobacco and tobacco products. The method is characterized by comprising the following steps of: digesting a sample by using acetonitrile; adding anhydrous magnesium sulfate, sodium chloride, sodium citrate and sodium hydrogen citrate to oscillate for layering; and subsequently, performing dispersive solid-phase extraction, and directly measuring the pesticide residues of 2,4-drop, 2,4,5-mucus and dicamba in the tobacco and tobacco products through liquid chromatogram-tandem mass spectrometry. According to the method, the defects of the sample treatment method in the prior art are overcome, and the sample pretreatment method and the instrument detection conditions are optimized aiming at the tobacco products; and compared with the prior art, the method has the beneficial effects that the sample pretreatment process is simple and rapid, derivatization is not required, and the method has the advantages that operation is accurate, sensitivity is high and repeatability is high.

The invention provides a method capable of simultaneously detecting varieties of pesticide residues in an aquatic product. The method capable of simultaneously detecting varieties of pesticide residues in the aquatic product comprises the following steps of extracting varieties of pesticide residues in the to-be-detected aquatic product, purifying a pesticide residue-containing extract, performing qualitative and quantitative analysis on the purifying liquid of the pesticide residue-containing extract through LC-MS / MS (liquid chromatography-mass spectrography) / (mass spectrography) and the like. According to the method capable of simultaneously detecting varieties of pesticide residues in the aquatic product, the function of separating and detecting all the pesticide residues by one step is realized, so that the efficiency of a sample pretreatment and analysis process is improved, the flexibility is high, and the detection cost is reduced to one tenth of the detection cost of the conventional method.

The invention discloses a method for simultaneously measuring residual quantity of three types of herbicide in vega soil, belonging to the technical field of physicochemical detection of pesticide residue in vega soil. Specifically, acetonitrile is used for extraction, Florisil is carried out with solid phase extraction, a gas chromatograph is matched with an electron capture detector to directly measure the residual quantity of three types of herbicide, such as propanil, Rimsulfuron, quizalofop-p-ethyl and the like in vega soil. The invention overcomes the restriction of the defect and the condition of the prior art to optimize the pretreatment method and the instrument detection condition of vega soil samples. Compared with the prior art, the invention has the following favorable advantages that the invention can detect three types of herbicide all at once and has the advantages of simple pretreatment process, high detection sensitivity and good accuracy and reproducibility.

The invention relates to a high performance liquid chromatography-tandem mass spectrometry measuring method of three amide herbicides (i.e., alachlor, metolachlor and napropamide) in tobacco and tobacco products, which belongs to the technical filed of physical and chemical inspection of pesticide residue in the tobacco and tobacco products. The high performance liquid chromatography-tandem mass spectrometry measuring method is characterized by being the method which is used for directly extracting the pesticide residue by a substrate dispersion solid-phase extraction method to directly measure the pesticide residue in the tobacco and tobacco products by virtue of chromatography-tandem mass spectrometry. The high performance liquid chromatography-tandem mass spectrometry measuring method of three amide herbicides in tobacco and tobacco products disclosed by the invention can be used for overcoming the defects of the sample treatment method in the prior art, and optimizing the sample pre-treatment method and the apparatus detection condition for the tobacco sample. Moreover, compared with the prior art, the method disclosed by the invention has the excellent effects that the sample pre-treatment process of the method is simple and quick, capable of reducing the interferences of the impurities including pigment and the like in the tobacco, and provided with the advantages of being accurate to operate, high in flexibility and good in repeatability.

The invention discloses a honeysuckle quality evaluation method. According to the honeysuckle quality evaluation method, chlorogenic acid, galuteolin and six iridoid glycoside ingredients in honeysuckle are determined, wherein the six iridoid glycoside ingredients are loganic acid, morroniside, loganin, chiratin, secoxyloganin and secologanin dimethyl acetal. The honeysuckle quality evaluation method disclosed by the invention has the advantages that the iridoid glycoside ingredients in the honeysuckle are included in a honeysuckle quality evaluation system for the first time, six representative iridoid glycoside ingredients in the honeysuckle are selected and determined at the same time for guaranteeing reliability and effectiveness of the quality evaluation method, and a dispersive solid phase extraction-high performance liquid chromatography (DSPE-HPLC) is established for the first time and used for detecting the six iridoid glycoside ingredients of different structures, so that reliability and effectiveness of honeysuckle quality evaluation are obviously improved, quality control level of traditional Chinese medicine honeysuckle and related patent medicines is further improved, and the honeysuckle quality evaluation method disclosed by the invention is significant on guidance of clinical application.

The present invention relates to an electrochemical test apparatus for the corrosion on a reclaimed water pipeline system in a flowing system. In the prior art, the traditional method can affect the specific process corrosion mechanism determination, and the static electrochemical test device cannot well simulate actual pipeline flowing condition. In order to overcome the defect in the prior art, the invention provides the long-term real-time nondestructive electrochemical test apparatus so as to assist the intermittent sampling test result, such that the corrosion mechanism of the reclaimed water pipeline system in the flowing system can be easily analyzed. The technical scheme of the present invention is that the electrochemical test apparatus comprises a nodular cast iron working electrode, an Ag / AgCl reference electrode, a platinum sheet auxiliary electrode having a purity of 99.99%, a supported annular reactor having a hole on the outer wall, and a motor. The apparatus and the method of the present invention are mainly used for the electrochemical test of the corrosion on the reclaimed water pipeline system.

The invention discloses a major QTL and SNP molecular marker for controlling Nelumbo nucifera color traits as well as a detection primer and application thereof. The invention firstly provides a majorQTL site for controlling the flower color traits of Nelumbo nucifera, and the major QTL site is positioned in a sixth linkage group and between two SNP markers; the major QTL is closely linked with the SNP molecular marker, has a relatively high contribution rate to the flower color traits of the Nelumbo nucifera, participates in regulating and controlling the phenotype of the red or white flowerof the Nelumbo nucifera, and can be used for map-based cloning mining of functional genes for controlling the flower color traits and molecular marker-assisted selection. The invention further provides an SNP molecular marker closely linked with the major QTL, and the SNP molecular marker is used for efficiently selecting Nelumbo nucifera varieties with target flower colors and molecular marker assisted breeding of Nelumbo nucifera with seeds and Nelumbo nucifera with roots. The invention further provides a PARMS primer group for detecting the SNP marker and a method for identifying the red and white flower traits of the Nelumbo nucifera, so that the flower color genotype data of the Nelumbo nucifera can be analyzed and obtained only through PCR amplification and without electrophoresis detection.

The invention discloses a method for synchronously extracting and purifying various chlorinated polycyclic aromatic hydrocarbons in soil, which belongs to the field of detection of the chlorinated polycyclic aromatic hydrocarbons. The method comprises the following steps: firstly removing moisture in a soil sample by virtue of a freezing dryer, grinding the soil sample, and filtering the ground soil sample by virtue of a 200-mesh sieve for later use; and extracting a target matter in the soil sample by utilizing an acceleration solvent extractor, purifying the target matter in a gel permeation chromatography, finally concentrating the target matter and sizing the target matter. According to the method, a preprocessing method of the chlorinated polycyclic aromatic hydrocarbons in the soil is established, less solvent is used in the entire process, the automation degree is high, less time is used, the safety is high, fewer steps are used, and various chlorinated polycyclic aromatic hydrocarbons in the soil can be accurately, qualitatively and quantitatively determined. The chlorinated polycyclic aromatic hydrocarbons in the soil are quantitatively detected by utilizing the gas phase chromatography-mass spectrometry, the detection limit is low (when S / N is equal to 3, the detection limit is 2.6 to 25pg / g), the precision is high (RSD=6.30 to 12.0 percent), the blank mark recovery rate is 64.1 to 109.2 percent, the synchronous analysis and detection of various trace chlorinated polycyclic aromatic hydrocarbons in the complicated environment medium such as the soil can be realized, sensitivity and accuracy can be realized, and the weaknesses of the prior art can be overcome.

The invention discloses a method for simultaneously measuring the residue contents of five weedicides with different structures in soil, and belongs to the technical field of physicochemical detection of pesticide residues in the soil. Specifically, the residue contents of three types of five weedicides with different structures in the soil are directly measured by a high-performance liquid chromatographic (HPLC) instrument by combining dispersive solid-phase extraction (DSPE) with dispersive liquid-liquid microextraction (DLLME). According to the method, the defects and the condition limitations in the prior art are overcome, a method for pre-treating a soil sample and the detection conditions of the instrument are optimized, a DSPE-DLLME-HPLC method is built for simultaneously detecting three types of five weedicides (metsulfuron methyl, bensulfuron methyl, prometryn, atrazine and quinclorac) with different structures in the soil, the linear correlation coefficients are all more than 0.99, the detection limit is 1.5-3.1 ngg<-1>, the average recovery rate is 74.9-90.1%, the relative standard deviation (RSD) is 4.0-5.7%, both the accuracy and the precision meet the residue detection requirements, and the method has the characteristics of low organic solvent consumption, good purification and concentration effects, high sensitivity, simplicity for performing and the like.

The invention relates to a detection and identification kit of a composite PCR of wheat stripe rust fungus and leaf rust fungus, which belongs to the technical fields of biotechnology, crop disease control and plant quarantine. The kit detects the specific genome DNA sequences of the wheat stripe rust fungus 171 bp and the leaf rust fungus 226 bp simultaneously in a primary PCR reaction by three primers from a fungal beta-tubulin gene conserved sequence and wheat stripe rust fungus and leaf rust fungus specific sequences. The invention provides supplement and improvement on the wheat stripe rust fungus and leaf rust fungus specific primer detecting method, and is suitable for the diagnostic detection of the stripe rust fungus and the leaf rust fungus in an infected wheat leaf before disease symptoms appear. Composite PCR technology is adopted in the kit, and in a PCR-SMART reaction solution, the three primers are amplified simultaneously, so the detection accuracy is increased, the detection time is saved, the operation is simple, and the kit can be applied and popularized at plant protection and plant quarantine departments in China.

The disclosed preparation method for an electrode chemical modified by O-carboxylmethychitose covalence bond comprises: dipping glass-carbon electrode into O-carboxylmethychitose-formic acid solution for modification, taking out to clean with formic acid and distilled water. This invention can improve detection sensitivity for DA, can eliminate effect of AA and UA, and has long lifetime.

The invention discloses a method for simultaneously measuring six iridoid glycoside ingredients in honeysuckle flowers. The six iridoid glycoside ingredients, namely, loganic acid, morroniside, loganin, chiratin, secoxyloganin and secologanin dimethyl acetal, of different structures in honeysuckle flowers are measured simultaneously based on a DSPE-HPLC method for the first time; by means of the method, the linearly dependent coefficients are larger than 0.999, the detection limit is 0.026-0.057 micrograms / mL, the recovery rates of all target ingredients range from 90.5% to 102.1%, the relative standard deviation (RSD) ranges from 0.85% to 1.74%, and the method has the advantages that the using amount of an organic solvent is small, the purification and concentration effects are good, the recovery rates of targets are high, the sensitivity is high, and operation is easy. The fast and efficient extraction, purification and analysis method suitable for iridoid glycoside ingredients in medical materials such as honeysuckle flowers is developed, and has great significance on further developing the acting mechanism of iridoid glycoside ingredients, establishing quality standards, increasing medical material and related patent medicine quality control levels and guiding clinical application.

The invention provides a method for determining the residual amount of three types of phenoxyalkanoic acid pesticides in tobaccos through an extraction-purification synchronization method. The methodis characterized by comprising the following steps: extracting a sample by utilizing a 0.5mol / L hydrochloric acid water solution; carrying out back extraction by utilizing dichloromethane; determiningthe residual amount of the pesticides including 2,4-D, 2,4,5-T and dicamba by utilizing an ultra performance liquid chromatography tandem mass spectrometry. According to the method provided by the invention, a standard working solution is prepared by utilizing a blank matrix and the influences on a matrix effect are overcome and the disadvantages of a sample treatment method in the prior art aremade up; a sample pre-treatment method and an instrument detection condition are optimized aiming at the tobacco sample; compared with the prior art, the method provided by the invention has the following good effects that a sample pre-treatment process of the method is simple and rapid and does not need derivatization; the method is green and environmentally friendly and has the advantages of accuracy in operation, high sensitivity and good repeatability.

The invention provides a method and device for measuring the absolute concentration of singlet-state oxygen (O2(al(delta)). The device comprises a static pool, an infrared detection system and a data acquisition system, wherein the infrared detection system is composed of a chopper, a lens, a narrow-band interference optical filter, an infrared detector and a phase-locked amplifier. In the invention, by monitoring the infrared radiation attenuation change of O2(al(delta)) at 1.27 microns in the static pool and combining the dynamic model analysis in the static pool, the absolute response coefficient of the infrared detection system can be automatically calibrated, and the absolute concentration of O2(al(delta)) can be further measured in real time. Moreover, the infrared attenuation method of the static pool can be applied to the real-time measurement for any flowing system containing O2(al(delta)) by use of a 'flowing-stopping' technology.

The invention discloses a mycobacterium tuberculosis IgG antibody enzyme-linked immunosorbent assay (ELISA) kit and preparation and application methods thereof. The mycobacterium tuberculosis IgG antibody ELISA kit comprises a TB-IgG enzyme label plate, a TB-IgG enzyme conjugate solution, a substrate solution A, a substrate solution B, a TB-IgG critical control solution, a TB-IgG positive control solution, a stop solution, a sample diluent and a concentrated detergent. The kit applies an indirect ELISA method for detecting a mycobacterium tuberculosis IgG antibody so as to reduce the interference of a false positive or false negative phenomenon in clinical diagnosis, the kit can be used for rapidly and accurately detecting mycobacterium tuberculosis, and meanwhile the kit is small in error, high in detection sensitivity and low in requirements on detection equipment.

The invention discloses a method for detecting residual quantity of disulfuron and metabolite thereof in plant-derived food, wherein the method comprises the steps: after oscillating and extracting a plant-derived food sample, carrying out dispersive solid-phase extraction and purification by taking a titanium carbide multilayer nanosheet as a purifying agent; and determining the residual quantity of the disulfuron and the metabolite thereof in the sample by adopting ultra-high performance liquid chromatography-tandem mass spectrometry. By optimizing a sample pretreatment method and instrument detection conditions, the pretreatment process is simple and rapid, the operation is accurate, the sensitivity is high, and the repeatability is good; and the sample recovery rate can reach 102.9%, and the relative standard deviation (RSD) is less than 6.0%.

The invention discloses a device for collecting intestinal flora, which belongs to the technical field of experimental devices and is designed to solve the problems of low sampling efficiency and the like in the existing method. The intestinal flora collection device of the present invention includes a binding layer located above and a receiving layer located below, the binding layer and the receiving layer are connected by connecting rods; a plurality of binding areas are arranged on the binding layer, and a plurality of binding areas are formed between two adjacent binding areas. Separation lines; each restraint area is provided with fixed stickers for restricting the activities of experimental animals. The intestinal flora collection device of the present invention can operate multiple experimental animals at the same time in one experiment, which improves the number of samples taken and the experimental efficiency; by restraining and fixing the experimental objects, the experimental operation is convenient and fast, and after the end of the experiment Afterwards, the experimental environment can also be cleaned conveniently; the intestinal flora collection device also provides a method for establishing an anxiety-induced mouse model in a biological experiment, and realizes the multifunctionality of the device.

The invention relates to a typing method for distinguishing different strains of cronobacter through performing single enzyme digestion on gluA gene based on RsaI, and belongs to the technical field of microbiological examination of food. Operation steps of the method disclosed by the invention are divided into two stages: 1, in the first stage, specific primer amplification on the cronobacter isperformed, so that gluA gene fragments of six different strains of the cronobacter are obtained; and 2, in the second stage, the specific gluA gene fragments of the cronobacter, which is amplified inthe first stage, are subjected to RsaI enzyme digestion, so that differential PCR-RFLP fingerprint graphs of the six different strains of the cronobacter are obtained, and the different strains of thecronobacter are further distinguished. A large quantity of examples of strains of the cronobacter verify that by adopting the molecular typing method, the differential enzyme digestion polymorphic graphs of the different strains can be effectively obtained, and the six different strains of the cronobacter can be quickly and accurately distinguished. The molecular typing method disclosed by the invention is used for distinguishing the strains of the cronobacter, and has an important practical meaning on accurately tracing pollution of the cronobacter in the food.

The invention discloses a method for detecting genotoxic impurities in an indobufen bulk drug. According to the method, octadecyl silane bonded silica gel or octyl bonded silica gel is used as a stationary phase for chromatographic separation of genotoxic impurities, a mass spectrum detector is used for analysis and detection, and the genotoxic impurities 2-(4-amino) phenylbutyric acid and / or 2-(4-nitro) phenylbutyric acid can be detected independently or simultaneously. According to the method, the genotoxic impurity 2-(4-amino) phenylbutyric acid and / or 2-(4-nitro) phenylbutyric acid can be independently or simultaneously and effectively separated, the content of the genotoxic impurity can be accurately determined, the specificity is high, the sensitivity is high, and the method has extremely important significance on quality control and safety guarantee of indobufen bulk drugs.

The invention discloses a tobacco and a measurement method for acibenzolar-S-methyl in the tobacco. The method is characterized by comprising the steps of completely decomposing the acibenzolar-S-methyl in a sample into metabolites (alarcic acid benzene) by using alkaline solution, acidizing by using hydrochloric acid, extracting by using acetonitrile, filtering, measuring the content of acibenzolar-S-methyl metabolites in the sample by liquid chromatography-tandem mass spectrometry, and thus indirectly measuring the content of the acibenzolar-S-methyl in the sample. According to the method provided by the invention, the defects of a sample processing method in the prior art is overcome, the blank of measurement on such kind of material is filled, many references can be provided for measurement of related residue limits and development of methods and techniques, a sample preparation method and an instrument detection condition are optimized aiming at the tobacco sample; compared with the prior art, the method provided by the invention has the following advantages of being simple and quick in sample preparation process, high in sensitivity and good in repeatability.

The invention belongs to the technical field of physicochemical testing of residual pesticide in tobacco and a tobacco product and in particular to a method for mensurating the residual amounts of six classes of dinitraniline pesticides, The method is to extract residual pesticides in an accelerated solvent extraction instrument (ASE), and use a gas chromatograph (matched with electron capture detector (ECD) to directly mensurate the residual amounts of six classes of the dinitraniline pesticides in the tobacco and the tobacco product, namely, butralin, flumetralin, isopropalin, pendimethalin, benfluralin and trifluralin. The method overcomes the disadvantages of a sample treatment method in the prior art and optimizes a sample pretreatment method and the instrument detecting conditions aiming at a tobacco sample. Compared with the prior art, the method has the advantages of simple pretreatment process, reduction of the interference of pigment and other impurities in the tobacco, highautomation degree of the whole operation, accurate operation, high sensitivity and good repeatability.

The invention provides a method for determining the residual amount of three phenoxycarboxylic acid pesticides in tobaccos by adopting an ultrahigh performance convergence chromatography-tandem mass spectrometry. The method is characterized by comprising the following steps: extracting a sample by utilizing a 0.5mol / L hydrochloric acid solution; carrying out back extraction by adopting dichloromethane and determining the residual amount of the pesticides including 2,4-D, 2,4,5-T and dicamba in the tobaccos by adopting the ultrahigh performance convergence chromatography-tandem mass spectrometry. By adopting the method provided by the invention, the disadvantages of a sample treatment method in the prior art are overcome; a sample pre-treatment method and instrument detection conditions aiming at tobacco samples are optimized; compared with the prior art, the method provided by the invention has the following good effects that a sample pre-treatment process of the method is simple and rapid and derivatization is not needed; the method is green and environmentally friendly and has the advantages of accuracy in operation, high sensitivity and good repeatability.

The invention discloses a composite PCR detection and identification kit for wheat stripe rust and leaf rust, belonging to the technical fields of biotechnology, crop disease prevention and control, and plant quarantine. The kit uses three primers derived from the conserved sequence of the fungal β-tubulin gene and the specific sequences of wheat stripe rust and leaf rust to simultaneously detect the specificity of wheat stripe rust 171bp and leaf rust 226bp in one PCR reaction. Genomic DNA sequence. It is a supplement and improvement to the specific primer detection method of wheat stripe rust and leaf rust, and is also suitable for the diagnostic detection of stripe rust and leaf rust in infected wheat leaves before disease symptoms appear. This kit adopts compound PCR technology. In the PCR-SMART reaction solution, three primers are simultaneously amplified, which improves the accuracy of detection, saves detection time, and is easy to operate. It can be popularized and applied by plant protection and plant inspection departments in my country.

The invention discloses a mycobacterium tuberculosis IgG antibody enzyme-linked immunosorbent assay (ELISA) kit and preparation and application methods thereof. The mycobacterium tuberculosis IgG antibody ELISA kit comprises a TB-IgG enzyme label plate, a TB-IgG enzyme conjugate solution, a substrate solution A, a substrate solution B, a TB-IgG critical control solution, a TB-IgG positive control solution, a stop solution, a sample diluent and a concentrated detergent. The kit applies an indirect ELISA method for detecting a mycobacterium tuberculosis IgG antibody so as to reduce the interference of a false positive or false negative phenomenon in clinical diagnosis, the kit can be used for rapidly and accurately detecting mycobacterium tuberculosis, and meanwhile the kit is small in error, high in detection sensitivity and low in requirements on detection equipment.

The invention provides a method for accurately detecting a T cell immune repertoire based on high-throughput sequencing and a primer system thereof, the primer system comprises a PCR1 primer and a PCR2 primer, the PCR1 primer sequence and the PCR2 primer sequence comprise an upstream V primer and a downstream J primer, and a sequencing linker sequence and a molecular tag sequence are respectively inserted into target areas of the upstream primer and the downstream primer of the PCR1; the PCR2 primer takes a PCR1 product as a template, and a sequencing primer sequence and an index tag are introduced. The primer system and the method for accurately detecting a T cell immune repertoire based on high-throughput sequencing have the characteristics of simplicity and convenience in sample treatment, rapidness in experimental operation and accurate quantification of TCR cloning clusters.

The invention discloses a method for evaluating the quality of honeysuckle. The method measures chlorogenic acid, luteolin and six iridoid glycoside components in honeysuckle, wherein the six iridoid glycoside components are Loganic acid, morroniside, loganin, dangdaoside, broken-oxyloganin, and bistroside dimethyl acetal. In the present invention, for the first time, the iridoid glycosides in honeysuckle are included in the quality evaluation system of honeysuckle, and in order to ensure the reliability and effectiveness of the quality evaluation method, six representative iridoid glycosides in honeysuckle are selected for determination , and for the first time established a dispersive solid phase extraction-high performance liquid chromatography (DSPE-HPLC) method to detect these six iridoid glycosides with different structures, which significantly improved the reliability and effectiveness of the quality evaluation of honeysuckle, thereby further It has improved the quality control level of honeysuckle medicinal materials and related finished medicines, which is of great significance for guiding clinical application.