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Tobacco and measurement method for acibenzolar-S-methyl in tobacco

A kind of technology of benzene and tobacco products, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of high sensitivity, simple experimental operation and accurate operation

Inactive Publication Date: 2019-01-18
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The object of the present invention is aimed at the current situation of complex tobacco matrix and many types of samples, overcome the defects of the prior art, provide a combination of liquid-liquid extraction and matrix matching method, and use liquid chromatography-tandem mass spectrometry to determine alaic acid in tobacco products Formula benzene-S-methyl metabolite content, thereby indirect determination method for the content of acitonic benzene-S-methyl, the method can quickly and accurately detect acitonic benzene-S-methyl residues in tobacco, determine Accurate results, less measurement interference

Method used

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  • Tobacco and measurement method for acibenzolar-S-methyl in tobacco
  • Tobacco and measurement method for acibenzolar-S-methyl in tobacco
  • Tobacco and measurement method for acibenzolar-S-methyl in tobacco

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example 1

[0031] 1. Instruments and reagents:

[0032] All pesticides are standard products; acetonitrile and formic acid are all pesticide residue grades.

[0033] API 4000 quadrupole tandem mass spectrometer; vortex oscillator (Labnet, USA); Sigma 3-30K centrifuge (Sigma, Germany); AE 163 electronic balance (sensitivity: 0.0001g) and AE 166 electronic balance (sensitivity : 0.01g) (Mettler, Switzerland).

[0034] 2. Sample handling:

[0035] Accurately weigh 1.0 g of sample (accurate to 0.01 g) into a 50 mL capped centrifuge tube. Add 18 mL of 0.2 mol / L sodium hydroxide solution, then place the centrifuge tube on a vortex mixer and vibrate at 2000 rpm for 2 min. Then place it in a water bath at 90°C for 30 min. Cool to room temperature, add 2.0 mL of 30% hydrochloric acid solution, mix well, then add 10 mL of acetonitrile and 7 g of sodium chloride, shake at 2000 rpm for 2 min. Aspirate 1mL of the supernatant, filter through a 0.45 μm organic phase filter, and detect with LC-MS...

example 2

[0041] Another tobacco sample was selected as described in Example 1, and the acibenzolar-S-methyl metabolite (araciphenone) was not detected in the sample.

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Abstract

The invention discloses a tobacco and a measurement method for acibenzolar-S-methyl in the tobacco. The method is characterized by comprising the steps of completely decomposing the acibenzolar-S-methyl in a sample into metabolites (alarcic acid benzene) by using alkaline solution, acidizing by using hydrochloric acid, extracting by using acetonitrile, filtering, measuring the content of acibenzolar-S-methyl metabolites in the sample by liquid chromatography-tandem mass spectrometry, and thus indirectly measuring the content of the acibenzolar-S-methyl in the sample. According to the method provided by the invention, the defects of a sample processing method in the prior art is overcome, the blank of measurement on such kind of material is filled, many references can be provided for measurement of related residue limits and development of methods and techniques, a sample preparation method and an instrument detection condition are optimized aiming at the tobacco sample; compared with the prior art, the method provided by the invention has the following advantages of being simple and quick in sample preparation process, high in sensitivity and good in repeatability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical inspection of pesticide residues in tobacco products, and mainly relates to the measurement technology of acitrin-S-methyl residues in tobacco. -Methyl groups are all decomposed into metabolites (aramic benzene), after acidification, are extracted with acetonitrile, filtered, and the content of araic benzene-S-methyl metabolites in the sample is determined by liquid chromatography-tandem mass spectrometry, thereby indirectly Determination of the content of acibenzolar-S-methyl in the sample. Background technique [0002] Tobacco is a smoking food, and its pesticide residue problem has become an important part of smoking safety issues. The pesticide residue index of tobacco and tobacco products is an important content in the quality control of tobacco products in various countries, and has become an important part of tobacco leaf evaluation and selection in the international market. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨飞范子彦李中皓刘珊珊王颖邓惠敏边照阳唐纲岭
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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