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A kind of Mycobacterium tuberculosis IgG antibody ELISA kit and its preparation and use method

A technology for immune detection and Mycobacterium tuberculosis, applied in the field of biological detection, can solve the problems of long culture time and low smear positive rate.

Active Publication Date: 2015-12-23
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although tuberculosis bacteriological examination is the "gold standard" for tuberculosis diagnosis, the smear-positive rate is low and culture takes a long time, which cannot meet the clinical needs, especially the diagnosis of bacterial negative pulmonary tuberculosis, extrapulmonary tuberculosis and childhood tuberculosis is very difficult, while immunology It is convenient, simple, fast, sensitive, and specific to check the source of specimens, and has become an important auxiliary inspection method for tuberculosis

Method used

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  • A kind of Mycobacterium tuberculosis IgG antibody ELISA kit and its preparation and use method

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Embodiment 1

[0070] A kind of Mycobacterium tuberculosis IgG antibody ELISA kit

[0071] Including TB-IgG microtiter plate, TB-IgG enzyme conjugate solution, substrate A solution, substrate B solution, TB-IgG critical control solution, TB-IgG positive control solution and stop solution, sample diluent, concentrated washing solution ;

[0072] There are 48 transparent reaction wells coated with solid-phase TB gene recombinant antigen on the TB-IgG microtiter plate;

[0073] The TB-IgG enzyme conjugate solution is horseradish-labeled mouse anti-human IgG antibody;

[0074] Each 1000ml of substrate A solution contains 4.16g of trisodium citrate, 7.76g of citric acid, 7.13g of sodium acetate, 1.52ml of glacial acetic acid, and 0.8ml of hydrogen peroxide;

[0075] Each 1000ml of substrate B solution contains 520ml of absolute ethanol, 272ml of ethylene glycol, 8ml of dimethylformamide, and 0.8g of 3,3',5,5'-tetramethylbenzidine;

[0076] Each 1000ml TB-IgG critical control solution contains ...

Embodiment 2

[0082] A kind of Mycobacterium tuberculosis IgG antibody ELISA kit

[0083] Including TB-IgG microtiter plate, TB-IgG enzyme conjugate solution, substrate A solution, substrate B solution, TB-IgG critical control solution, TB-IgG positive control solution and stop solution, sample diluent, concentrated washing solution ;

[0084] There are 48 transparent reaction wells coated with solid-phase TB gene recombinant antigen on the TB-IgG microtiter plate;

[0085] The TB-IgG enzyme conjugate solution is horseradish-labeled mouse anti-human IgG antibody;

[0086] Each 1000ml of substrate A solution contains 6.24g of trisodium citrate, 11.64g of citric acid, 10.70g of sodium acetate, 2.28ml of glacial acetic acid, and 1.2ml of hydrogen peroxide;

[0087] Each 1000ml substrate B solution contains 780ml of absolute ethanol, 408ml of ethylene glycol, 12ml of dimethylformamide, 1.2g of 3,3',5,5'-tetramethylbenzidine;

[0088] Each 1000ml TB-IgG critical control solution contains 8g o...

Embodiment 3

[0094] A kind of Mycobacterium tuberculosis IgG antibody ELISA kit

[0095] Including TB-IgG microtiter plate, TB-IgG enzyme conjugate solution, substrate A solution, substrate B solution, TB-IgG critical control solution, TB-IgG positive control solution and stop solution, sample diluent, concentrated washing solution ;

[0096] There are 48 transparent reaction wells coated with solid-phase TB gene recombinant antigen on the TB-IgG microtiter plate;

[0097] The TB-IgG enzyme conjugate solution is horseradish-labeled mouse anti-human IgG antibody;

[0098] Each 1000ml of substrate A solution contains 5.2g of trisodium citrate, 9.7g of citric acid, 8.92g of sodium acetate, 1.9ml of glacial acetic acid, and 1ml of hydrogen peroxide;

[0099] Each 1000ml substrate B solution contains 650ml of absolute ethanol, 340ml of ethylene glycol, 10ml of dimethylformamide, 1g of 3,3',5,5'-tetramethylbenzidine;

[0100] Each 1000ml TB-IgG critical control solution contains 7g of sodium ...

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Abstract

The invention discloses a mycobacterium tuberculosis IgG antibody enzyme-linked immunosorbent assay (ELISA) kit and preparation and application methods thereof. The mycobacterium tuberculosis IgG antibody ELISA kit comprises a TB-IgG enzyme label plate, a TB-IgG enzyme conjugate solution, a substrate solution A, a substrate solution B, a TB-IgG critical control solution, a TB-IgG positive control solution, a stop solution, a sample diluent and a concentrated detergent. The kit applies an indirect ELISA method for detecting a mycobacterium tuberculosis IgG antibody so as to reduce the interference of a false positive or false negative phenomenon in clinical diagnosis, the kit can be used for rapidly and accurately detecting mycobacterium tuberculosis, and meanwhile the kit is small in error, high in detection sensitivity and low in requirements on detection equipment.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an ELISA kit for Mycobacterium tuberculosis IgG antibody and a preparation and use method thereof. Background technique [0002] Mycobacterium tuberculosis (M.tuberculosis) is referred to as tuberculosis (tuberclebacilli, TB). As early as 1886, German bacteriologist Koch had proved that TB is the pathogenic bacteria of tuberculosis. The bacteria can invade various tissues and organs of the whole body, but the most common infection is the lungs. With the continuous development of anti-tuberculosis drugs and the improvement of sanitary living conditions, the morbidity and mortality of tuberculosis once dropped sharply. After the 1980s, due to factors such as the emergence of AIDS and TB drug-resistant strains, the application of immunosuppressants, drug abuse, poverty, and population mobility, the epidemic situation of tuberculosis worldwide deteriorated suddenly. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543
Inventor 杨致亭王好玉邓旭刘树生刘兆军杨爱香
Owner 山东康华生物医疗科技股份有限公司
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