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Mycobacterium tuberculosis IgG antibody enzyme-linked immunosorbent assay (ELISA) kit and preparation and application methods thereof

A technology for immune detection and Mycobacterium tuberculosis, applied in the field of biological detection, can solve the problems of long time-consuming culture and low positive rate of smear

Active Publication Date: 2013-09-25
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although tuberculosis bacteriological examination is the "gold standard" for tuberculosis diagnosis, the smear-positive rate is low and culture takes a long time, which cannot meet the clinical needs, especially the diagnosis of bacterial negative pulmonary tuberculosis, extrapulmonary tuberculosis and childhood tuberculosis is very difficult, while immunology It is convenient, simple, fast, sensitive, and specific to check the source of specimens, and has become an important auxiliary inspection method for tuberculosis

Method used

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  • Mycobacterium tuberculosis IgG antibody enzyme-linked immunosorbent assay (ELISA) kit and preparation and application methods thereof

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Experimental program
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Effect test

Embodiment 1

[0070] Enzyme-linked immunosorbent assay kit for tuberculosis IgG antibody

[0071] Including TB-IgG enzyme label plate, TB-IgG enzyme conjugate solution, substrate A solution, substrate B solution, TB-IgG critical control solution, TB-IgG positive control solution and stop solution, specimen diluent, concentrated washing solution ;

[0072] The TB-IgG microtiter plate is equipped with 48 transparent reaction wells coated with solid-phase TB gene recombinant antigen;

[0073] TB-IgG enzyme conjugate solution is horseradish-labeled mouse anti-human IgG antibody;

[0074] Each 1000ml substrate A solution contains 4.16g trisodium citrate, 7.76g citric acid, 7.13g sodium acetate, 1.52ml glacial acetic acid, 0.8ml hydrogen peroxide;

[0075] Each 1000ml of substrate B solution contains 520ml of absolute ethanol, 272ml of ethylene glycol, 8ml of dimethylformamide, 0.8g of 3,3',5,5'-tetramethylbenzidine;

[0076] Every 1000ml TB-IgG critical control solution contains 6g of sodium chloride and ...

Embodiment 2

[0082] Enzyme-linked immunosorbent assay kit for tuberculosis IgG antibody

[0083] Including TB-IgG enzyme label plate, TB-IgG enzyme conjugate solution, substrate A solution, substrate B solution, TB-IgG critical control solution, TB-IgG positive control solution and stop solution, specimen diluent, concentrated washing solution ;

[0084] The TB-IgG microtiter plate is equipped with 48 transparent reaction wells coated with solid-phase TB gene recombinant antigen;

[0085] TB-IgG enzyme conjugate solution is horseradish-labeled mouse anti-human IgG antibody;

[0086] Each 1000ml substrate A solution contains 6.24g trisodium citrate, 11.64g citric acid, 10.70g sodium acetate, 2.28ml glacial acetic acid, 1.2ml hydrogen peroxide;

[0087] Each 1000ml of substrate B solution contains 780ml of absolute ethanol, 408ml of ethylene glycol, 12ml of dimethylformamide, 1.2g of 3,3',5,5'-tetramethylbenzidine;

[0088] Each 1000ml TB-IgG critical control solution contains 8g of sodium chloride an...

Embodiment 3

[0094] Enzyme-linked immunosorbent assay kit for tuberculosis IgG antibody

[0095] Including TB-IgG enzyme label plate, TB-IgG enzyme conjugate solution, substrate A solution, substrate B solution, TB-IgG critical control solution, TB-IgG positive control solution and stop solution, specimen diluent, concentrated washing solution ;

[0096] The TB-IgG microtiter plate is equipped with 48 transparent reaction wells coated with solid-phase TB gene recombinant antigen;

[0097] TB-IgG enzyme conjugate solution is horseradish-labeled mouse anti-human IgG antibody;

[0098] Each 1000ml of substrate A solution contains 5.2g of trisodium citrate, 9.7g of citric acid, 8.92g of sodium acetate, 1.9ml of glacial acetic acid, and 1ml of hydrogen peroxide;

[0099] Each 1000ml of substrate B solution contains 650ml of absolute ethanol, 340ml of ethylene glycol, 10ml of dimethylformamide, and 1g of 3,3',5,5'-tetramethylbenzidine;

[0100] Each 1000ml TB-IgG critical control solution contains 7g of s...

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Abstract

The invention discloses a mycobacterium tuberculosis IgG antibody enzyme-linked immunosorbent assay (ELISA) kit and preparation and application methods thereof. The mycobacterium tuberculosis IgG antibody ELISA kit comprises a TB-IgG enzyme label plate, a TB-IgG enzyme conjugate solution, a substrate solution A, a substrate solution B, a TB-IgG critical control solution, a TB-IgG positive control solution, a stop solution, a sample diluent and a concentrated detergent. The kit applies an indirect ELISA method for detecting a mycobacterium tuberculosis IgG antibody so as to reduce the interference of a false positive or false negative phenomenon in clinical diagnosis, the kit can be used for rapidly and accurately detecting mycobacterium tuberculosis, and meanwhile the kit is small in error, high in detection sensitivity and low in requirements on detection equipment.

Description

Technical field [0001] The invention relates to the technical field of biological detection, in particular to a tubercle bacillus IgG antibody enzyme-linked immunoassay detection kit and a preparation and use method thereof. Background technique [0002] Mycobacterium tuberculosis (M. tuberculosis) is referred to as tubercle bacilli (TB). As early as 1886, German bacteriologist Koch had proved that TB was the pathogen of tuberculosis. This bacterium can invade all tissues and organs of the whole body, but the lung infection is most common. With the continuous development of anti-tuberculosis drugs and the improvement of health and living conditions, the morbidity and mortality of tuberculosis once dropped significantly. After the 1980s, due to the emergence of AIDS and TB drug-resistant strains, the application of immunosuppressants, drug abuse, poverty, and population migration, the global tuberculosis epidemic has suddenly worsened. According to WHO statistics, about 1 in 3 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
Inventor 杨致亭王好玉邓旭刘树生刘兆军杨爱香
Owner 山东康华生物医疗科技股份有限公司
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