The invention relates to an application of
citrobacter rodentium for circularly preparing biological flocculants by using waste
algae, and belongs to a method for treating algal blooms. The application of the
citrobacter rodentium for circularly preparing the biological flocculants by using the waste
algae comprises the following steps of performing fermental cultivation on the
citrobacter rodentium to obtain
fermentation liquor under the condition that the
inoculation amount accounts for 0.5-2% of the total weight of a
fermentation medium; centrifugally removing
mycelium to obtain liquid supernatant; adding ethyl
alcohol of which the volume of the ethyl
alcohol is twice greater than that of the liquid supernatant and standing for 12 hours at the temperature of 4 DEG C; centrifuging mixed liquid and dissolving obtained 3-5g of sediments in 100ml of deionized water; adding 2% of cetyl
pyridinium chloride with the volume of 50ml in the deionized water and vibrating for 3-4 hours; collecting sediments and dissolving the sediments in 0.5M of NaCl liquor with the volume of 100ml; adding ethyl
alcohol of which the volume is twice greater than the NaCl liquor; centrifuging and collecting sediments; eluting the collected sediments by using the ethyl alcohol and dissolving the sediments in 5ml of deionized water; and performing
vacuum drying to obtain a product. The application of the citrobacter rodentium for circularly preparing the biological flocculants by using the waste
algae has the advantages that the production cost of the biological flocculants is greatly reduced, and secondary
pollution cannot be caused; and the citrobacter rodentium is easily degraded by microorganisms, is non-poisonous and harmful, is high in safety, and is beneficial to application and popularization of the biological flocculants.