Preparation method of compound flocculating agent for adsorbing metal ions
A technology of metal ions and flocculants, applied in chemical instruments and methods, flocculation/precipitation water/sewage treatment, separation of microorganisms, etc., can solve the problems of low activity, poor adsorption, and low utilization rate of flocculants, and achieve high-efficiency flocculation, The effect of improving utilization and activity
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[0029] A preparation method of a composite type adsorption metal ion flocculant, comprising the steps of:
[0030] (1) Take the sludge and add it to a beaker with deionized water at a mass ratio of 1:5, place it on a magnetic stirrer and stir for 10 minutes, let it stand for 1~2 hours, centrifuge, collect the supernatant, and take the mass of the supernatant Four equal parts were inoculated into No. 1 bacterial liquid medium, No. 2 mold liquid medium, No. 3 actinomycete liquid medium, and No. 4 fungal liquid medium respectively according to the mass ratio of 2:9, and cultivated at 25~33°C for 3 For ~5 days, according to the same inoculum size and the same culture conditions, repeat the enrichment culture twice to obtain No. 1, No. 2, No. 3 and No. 4 enrichment culture solutions, and use 0.9% normal saline to No., No. 2, No. 3, and No. 4 enriched culture medium were diluted to 10 -6 Dilution, after dilution, No. 1, No. 2, No. 3, and No. 4 enriched culture fluids were obtained,...
example 1
[0035] Raw material for flocculant extraction: take the sludge in the river water and store it at 4°C.
[0036] No. 1 Bacterial Solid Medium: In terms of parts by mass, take 3 parts of beef extract, 6 parts of peptone, 15 parts of agar, 2 parts of NaCl, 1000 parts of water, pH 7, sterilized at 121°C for 20 minutes.
[0037] No. 2 mold solid medium: in parts by mass, take 20 parts of sucrose, NaNO 3 1 copy, K 2 HPO 4 0.5 parts, MgSO 4 0.2 parts, FeSO 4 0.01 parts, 13 parts of agar, 1000 parts of water, pH 7, sterilized at 115°C for 20 minutes.
[0038] No. 3 actinomycetes solid medium: by mass parts, take 18 parts of soluble starch, KNO 3 0.6 parts, K 2 HPO 4 0.3 parts, MgSO 4 ·7H 2 O0.2 parts, FeSO 4 ·7H 2 0.02 parts of O, 0.4 parts of NaCl, 15 parts of agar, 1000 parts of water, pH 7, sterilized at 121°C for 20 minutes.
[0039] No. 4 fungal solid medium: in parts by mass, 180 parts of potatoes, 20 parts of sucrose, 18 parts of agar, 1000 parts of water, natural p...
example 2
[0049] Raw material for flocculant extraction: take the sludge in the river water and store it at 4°C.
[0050] No. 1 Bacterial Solid Medium: In terms of parts by mass, take 5 parts of beef extract, 10 parts of peptone, 20 parts of agar, 3 parts of NaCl, 1000 parts of water, pH 7.4, and sterilize at 121°C for 20 minutes.
[0051] No. 2 mold solid medium: by mass parts, take 25 parts of sucrose, NaNO 3 2 copies, K 2 HPO 4 0.8 parts, MgSO 4 0.3 parts, FeSO 4 0.03 parts, 15 parts of agar, 1000 parts of water, pH7.2, sterilized at 115°C for 20min.
[0052] No. 3 actinomycetes solid medium: in parts by mass, take 20 parts of soluble starch, KNO 3 1 copy, K 2 HPO 4 0.6 parts, MgSO 4 ·7H 2 O0.5 parts, FeSO 4 ·7H 2 0.03 parts of O, 0.7 parts of NaCl, 20 parts of agar, 1000 parts of water, pH 7.4, sterilized at 121°C for 20 minutes.
[0053] No. 4 fungal solid medium: in parts by mass, 200 parts of potatoes, 22 parts of sucrose, 20 parts of agar, 1000 parts of water, natural ...
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