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Pseudomonas PR1 as well as preparation method and application thereof

A technology of Pseudomonas and culture method, applied in the field of Pseudomonas PR1 and its preparation, can solve problems such as difficulty in balancing flocculation efficiency and secondary pollution

Active Publication Date: 2021-06-25
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common flocculants include inorganic flocculants and organic polymer flocculants. Although common inorganic flocculants can remove non-ferrous metal ions in wastewater containing non-ferrous metals, they can easily cause secondary pollution
Although flocculants made of microorganisms can avoid secondary pollution, it is difficult to balance the efficiency of flocculation

Method used

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  • Pseudomonas PR1 as well as preparation method and application thereof
  • Pseudomonas PR1 as well as preparation method and application thereof
  • Pseudomonas PR1 as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Screening of bacteria

[0047] (1) Preparation of medium:

[0048] (A) Separation medium

[0049] Beef extract peptone (g / L): beef extract 3.0, peptone 10.0, sodium chloride 5.0 and agar 20.0, pH=7.0;

[0050] LB medium (g / L): peptone 10.0, yeast extract 5.0, sodium chloride 6.0 and agar 20.0, pH=7.0.

[0051] (B) Fermentation medium (g / L): glucose 10.0, potassium dihydrogen phosphate 0.5, dipotassium hydrogen phosphate 5.0, ammonium sulfate 0.2, sodium chloride 0.1, urea 0.5, yeast extract 0.5 and magnesium sulfate 0.2, pH=7 .

[0052] (C) Seed medium (g / L): glucose 10.0, potassium dihydrogen phosphate 0.5, dipotassium hydrogen phosphate 5.0, ammonium sulfate 0.2, sodium chloride 0.1, urea 0.5, yeast extract 0.5 and magnesium sulfate 0.2, pH=7 .

[0053] The above-mentioned culture medium was sterilized at 120°C for 30 minutes and left for 24 hours before use if there is no contamination.

[0054] (2) Bacterial source preparation: The soil was collected from lead...

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Abstract

The invention belongs to the technical field of wastewater treatment, and particularly relates to a pseudomonas PR1 as well as a preparation method and application thereof. The invention provides a strain of pseudomonas PR1, and the preservation number of the pseudomonas PR1 is GDMCC (China General Microbiological Culture Collection Center) NO: 61255. The pseudomonas PR1 provided by the invention has the effect of efficiently flocculating nonferrous metal ions. The flocculating agent containing the pseudomonas PR1 is added into the non-ferrous metal wastewater containing Pb < 2 + > and / or Zn < 2 + >, so that an efficient flocculation effect can be achieved, and the flocculation rate of Pb < 2 + >is 99.35%; the flocculation rate of Zn < 2 + > is 55.90%; in the mixed wastewater of Pb < 2 + > and Zn < 2 + >, the flocculation rate of Pb < 2 + > is 92.94%, and the flocculation rate of Zn < 2 + > is 80.67%.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment, and in particular relates to a strain of pseudomonas PR1 and its preparation method and application. Background technique [0002] Non-ferrous metal resources are important strategic resources, but the mining process can easily pollute the environment and even affect people's health. For example, the accumulation of lead in the body of humans and animals will not only affect the health of the body and even cause death; excessive intake of zinc will cause gastrointestinal discomfort, and seriously damage the liver function to a certain extent. [0003] The flocculation method is widely used in the wastewater treatment of lead-zinc metal flotation beneficiation. Common flocculants include inorganic flocculants and organic polymer flocculants. Although common inorganic flocculants can remove non-ferrous metal ions in wastewater containing non-ferrous metals, they can easily cause secon...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F1/52C02F3/34C02F101/20C12R1/38
CPCC12N1/20C02F1/52C02F3/34C02F2101/20
Inventor 陆燕勤裴润全曾鸿鹄史成科
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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