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Microbial flocculant and dicyandiamide formaldehyde resin combined decoloring agent, and preparation and application methods thereof

A technology of microbial flocculant and formaldehyde resin, applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of environmental ecological hazards and high costs

Active Publication Date: 2014-03-26
东莞市永佳合成材料有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, although organic polymer decolorizers can effectively reduce chroma, there are still problems of high cost and potential environmental and ecological hazards

Method used

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  • Microbial flocculant and dicyandiamide formaldehyde resin combined decoloring agent, and preparation and application methods thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 microbial flocculant

[0035] Pannonibacter phragmitetus B7 (CCTCC M2013025) strain was sampled and isolated from the activated sludge of the printing and dyeing wastewater plant. After 72 hours of culture on LB medium, it formed light yellow and viscous colonies. Through physiological and biochemical experiments, it was shown that the bacteria were Gram-negative bacteria. The bacteria can secrete and express extracellular polymers, which can be used as flocculants in the pretreatment of fermentation broth. There is no report on the use of Pannonibacter phragmitetus bacteria to obtain microbial flocculants.

[0036] 1) Fermentation and cultivation of flocculant-producing strains

[0037] Weight composition of seed medium (g / L):

[0038]Slant solid medium: beef extract 5, peptone 10, water to 1000mL, agar 15-20, pH7.4-7.8. Seed liquid medium: beef extract 5, peptone 10, water to 1000mL, pH7.4-7.8;

[0039] Weight composition of ferment...

Embodiment 2

[0046] Example 2 Combined treatment of simulated printing and dyeing wastewater by dicyandiamide formaldehyde resin decolorizer and microbial flocculant

[0047] Simulated printing and dyeing wastewater: reactive yellow 3RS0.0075g / L; reactive red 3BS0.03g / L; reactive blue KNB0.0125g / L; baking soda 7g / L; salt (sodium chloride) 20g / L; 10.77; the chromaticity is 1100 times.

[0048] Take 50mL of simulated printing and dyeing wastewater into a small beaker, add 0.2mL of 500ppm dicyandiamide formaldehyde resin decolorizer respectively, place it on a magnetic stirrer and stir for 1min, then add 1-1.5mL of 1g / L microbial flocculant again, and stir for 1min; After standing still for 3 min, the supernatant was taken to measure the absorbance value at a wavelength of 540 nm. Using the simulated printing and dyeing wastewater as a control, calculate the decolorization rate (decolorization rate=(A-B) / A*100%, A: the absorbance value of the supernatant after treatment, B: the absorbance va...

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Abstract

The invention provides a microbial flocculant and dicyandiamide formaldehyde resin combined decoloring agent, and preparation and application methods thereof, comprising fermentation cultivation of a Pannonibacter phragmitetus B7. Strain (which has been collected in China Center for Type CultureCollection (CCTCC) and has the collection number of CCTCC NO: M2013025), extraction and preparation of a microbial flocculant, the microbial flocculant and dicyandiamide formaldehyde resin combined decoloring agent, and a treatment method of the combined decoloring agent in printing and dyeing waste water. The dicyandiamide formaldehyde resin is provided with high positive charges, thus being effectively combined with residual dye in the printing and dyeing waste water. The microbial flocculant is distributed in the supernatant of fermentation liquid, the fermentation process is simple, and extraction and preparation of the microbial flocculant are convenient. Due to combined utilization of the dicyandiamide formaldehyde resin and the microbial flocculant, a good synergistic effect is achieved and the decoloring rate can at least reach 90 percent.

Description

technical field [0001] The present invention relates to a combination of microbial flocculant and dicyandiamide formaldehyde resin decolorizer and its preparation and application method, a method for combined treatment of printing and dyeing wastewater, specifically refers to a method using Pannonibacter phragmitetus B7. Center, the preservation number is: CCTCC M2013025) produced microbial flocculant and dicyandiamide formaldehyde resin combined decolorizer, its preparation method and new process for treating printing and dyeing wastewater. Background technique [0002] In the discharge and treatment of various industrial wastewater, printing and dyeing wastewater is one of the main harmful and difficult-to-treat industrial wastewater in my country. It is characterized by large discharge volume, deep color, high content of organic pollutants, and high frequency of water quality changes. The pollution of dyestuff is the most serious. With the rapid development of the dye ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04C02F3/34C12R1/01C02F103/30
Inventor 彭彦周洪波田健
Owner 东莞市永佳合成材料有限公司
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