31results about How to "Economic gain" patented technology

The invention provides a harvesting method of microalgae mediated by fungus. The method is characterized by comprising the following steps: (1) transferring microalgae; (2) culturing the microalgae in high density; (3) culturing spores of fungus capable of balling; (4) adding fresh spore suspension into a microalgae culture medium; (5) forming a fungus-mediated large-grained fungus-algae homobium; and (6) harvesting the large-grained bacillus-algae homobium by a filtration method. According to the method, the fungus strain capable of balling is introduced and cultured in a mixing way in the microalgae culture medium to be harvested, so that the aim of symbiosis and balling of fungus and microalgae can be achieved, and the fungus-mediated large-grained fungus-algae homobium can be harvested by virtue of a simple filtration method.

The invention discloses a method for rapidly constructing an siRNA carrier by using pSilencer plasmid. The steps include the construction of subcloning vector, the synthesis method of siRNA used in the rapid screening process, the rapid screening method of positive clones containing siRNA, and the like. Compared with conventional vector construction methods, this method is more effective, faster, and more economical to obtain the required small-scale gene vectors, and is especially suitable for functional verification analysis of large-scale gene screening results such as gene chips, SSH, and SAGE. , and research on the search for effective inhibitory sequence siRNA and other RNA interference mechanisms.

The invention provides a cell model and a screening method for screening a calcium-activated chloride ion channel inhibitor. The screening method sequentially comprises the steps of (1) constructing eukaryotic expression vectors of Anoctamin1 or Anocatmin2 and YFP-H148Q / I152L; (2) stably transfecting the two vectors into FRT (Fisher rat thyroid) cells respectively to ensure that the RFT cells co-express two proteins of YFP-H148Q / I152l and Anoctamin1 or Anocatmin2, and performing multiple times of limiting dilution to obtain an FRT cell model; (3) transferring the FRT cell model to a black-wall microporous plate with a black wall and a transparent bottom; (4) adding a small molecular compound to be detected into the black-wall microporous plate, incubating, then adding a regent for raising the concentration of calcium ions in cells and high-concentration iodide ions, and screening the calcium-activated chloride channel inhibitor by detecting the change of relative fluorescence intensity.

The invention provides a satellite remote sensing monitoring method for the total chlorophyll content of the canopies of jujube trees. The satellite remote sensing monitoring method for the total chlorophyll content of the canopies of the jujube trees includes the following steps: determining a region to be monitored; downloading a satellite remote sensing image of the region to be monitored; successively carrying out geometric precision correction, radiation correction and atmospheric correction on the downloaded satellite remote sensing image; cutting the satellite remote sensing image after the satellite remote sensing image is subjected to atmospheric correction to obtain an image of a monitored region; calculating 6 vegetation indexes of selected picture elements of the monitored region by software ENVI 5.1; substituting the 6 vegetation indexes into a model through band calculation, and calculating the total chlorophyll content of the canopies of the jujube trees in each picture element, wherein Y is the total chlorophyll content of the canopies of the jujube trees, and the unit of the total chlorophyll content is mg / g. By the method, the total chlorophyll content data of the canopies of the jujube trees can be rapidly, accurately and economically obtained in an environment-friendly manner in regional scale, environmental pollution and injury to human bodies due to the discharge of chemical tail liquid in a measuring process are avoided, operation steps are greatly simplified, and monitoring time is shortened.

The present invention relates to an antigenic B-cell multi-epitope peptide linked in tandem in the outer membrane protein(Omp) U gene of vibrio mimicus, a making method and an application thereof, which belong to the field of molecular immunology. The B-cell multi-epitope peptide linked in tandem can induce fish to make protective immunity response to vibrio mimicus infection. In the present invention, the amino acid sequence of the antigenic B-cell multi-epitope peptide linked in tandem in the OmpU is shown in SEQIDNO:10. The B-cell multi-epitope peptide linked in tandem in the OmpU of vibrio mimicus is made by a genetic engineering technique. The verification of immune blotting analysis, specific antibody detection and immune animal protective experiments shows that the peptide can elicit efficient and specific protective humoral immunity response to vibrio mimicus infection. The B-cell multi-epitope peptide linked in tandem can be used for the immune diagnosis and the immune prevention and treatment of aquatic animal ascitic diseases. The present invention has high social benefit and economic benefit.