The invention relates to a micro-fluidic
chip, a kit and an application method of the kit. A base body of the micro-fluidic
chip is provided with a positioning groove, a sample cavity, a
lysis solution storage cavity, a sample
lysis cavity, a cleaning solution storage cavity, an eluent storage cavity, a buffer cavity, a
nucleic acid cavity, a waste liquid cavity, a distribution pipeline and at least two PCR reaction cavities; and the base body is further provided with a
protease solution storage cavity, a mixing cavity, a filtering area and a selection cavity. The number of PCR reaction cavities can be set when the
chip is applied to detection; a
fluorescent PCR detection method is combined with a micro-fluidic technology, the advantages of the two technologies are complementary, the defects of the two technologies are avoided, the possibility of leakage of samples or chemical reagents is avoided, the possibility of
nucleic acid pollution is greatly reduced, related pathogenic bacteriain the samples can be quickly and accurately identified, the kit is particularly suitable for realizing multiple detection, and the experimental time and cost are saved; and the requirements on operation environment, personnel skills and the like are low, the
automation degree is high, the consumed time is short, and the performance indexes such as specificity and sensitivity of detection can beconsidered.