53 results about "Primary metabolism" patented technology

The invention discloses a GC-MS-based plant non-targeted metabolomics sample pretreatment method. The method comprises the steps of 1) mixing up a plant sample, an internal standard substance and a hydrophilic organic solvent, wherein the hydrophilic organic solvent is pre-cooled to be -20 to 4 DEG C; cooling the obtained mixture to be -80 to -10 DEG C, grinding, crushing and conducting the supersonic extraction in the ice-water bath; 2) then respectively adding a lipophilic organic solvent and water, uniformly mixing up, conducting the supersonic extraction in the ice-water bath, conducting the high-speed centrifugation at the temperature of 0 to 16 DEG C, obtaining the aqueous phase and evaporating; 3) adding an oximation reagent, and conducting the oximation reaction at the temperature of 30 to 45 DEG C; 4) finally adding a derivatization reagent and n-hexane, and conducting the derivatization reaction at the temperature of 60 to 80 DEG C. According to the technical scheme of the invention, based on the above pretreatment method, the primary metabolites of a plant sample can be fully extracted, so that the abundant metabolite spectrum data information can be obtained. Meanwhile, both the change of metabolites during the extraction process, and the pollution of liposoluble substances to a gas chromatographic column can be avoided as much as possible. Therefore, the better sample reproducibility is realized.

The invention provides a method for detecting primary metabolites and secondary metabolites in fresh tobacco leaves with GC-MS (gas chromatography-mass spectrometer). The method comprises steps as follows: the fresh tobacco leaves are rapidly collected and quick-frozen with liquid nitrogen on site, the quick-frozen tobacco leaves are transferred to dry ice and transported to a laboratory at low temperature, moisture is removed by a freeze dryer with a low-temperature freeze drying method, the tobacco leaves are pulverized by a pulverizer and screened by a 40-mesh sieve, the primary metabolites and the secondary metabolites of the pulverized tobacco leaves are extracted ultrasonically with a solvent, a supernatant of an extracting solution is subjected to nitrogen blow-drying, blow-dried extracts are subjected to an oximation reaction and a silane derivatization reaction, and GC-MS analysis is performed finally. The method has the advantages as follows: a tobacco leaf collecting method can really reflect growth and development periods, collecting parts, varieties and production places of the tobacco leaves; a tobacco leaf extracting and detecting method can cover the primary and secondary metabolites such as sugar, amino acid, organic acid, sterol, alkaloid, polyphenol and the like of the fresh tobacco leaves and has high-flux detection characteristics, and a selective ion monitoring method can realize accurately qualitative and quantitative analysis of 199 primary and secondary metabolites in the fresh tobacco leaves, and 135 metabolites are required to be verified in a standard substance.

The invention provides haptens, immunogens comprising such haptens coupled to an antigenicity-conferring carrier material, conjugates comprising such haptens bonded to a labelling agent as well as, antibodies raised against such immunogens and capable of binding with ketamine and its primary metabolite, norketamine.

The present invention relates to a method to improve the primary metabolism of a higher plant having chloroplasts by expressing fructose-1,6-bisphosphatase / sedoheptulose-1,7-bisphosphatase derived from Cyanobacterium synechococcus in the chloroplasts. The invention further relates to transgenic plants comprising DNA encoding fructose-1,6-bisphosphatase / sedoheptulose-1,7-bisphosphatase derived from Cyanobacterium synechococcus.

The technological scheme of the present invention is that taurine is added into beer to produce functional taurine beer. The production process includes saccharification of beer wort, filtering, adding hop, boiling, separating hop from beer, cooling, adding taurine, filtering to obtain beer product. The added amount of taurine is 0.010-0.050 wt%. The functional taurine beer can lower cholesterol, resisting lipoid peroxidation and prevent blood fat raising, and the taurine can excite the activity of acetaldehyde dehydrogenase so as to lower or remit the inhibition of acetaldehyde as the primary metabolism product of alcohol in beer on human body's central nerve system and to protect liver and stomach mucous membrane.

The invention provides a method for cloning a saccharopolyspora erythraea expression plasmid (pBlueV) containing a vitreoscilla hemoglobin gene (vgb), belonging to the technical field of gene engineering. In the method, based on the fact that VHb (hemoglobin) can be combined with oxygen to form an oxygenated state, the VHb intervenes the oxygen related metabolic pathway of cells, thereby changing the original biological metabolism mode of the cells under oxygen-limited conditions, promoting the cell growth and protein synthesis under oxygen-deficient conditions, directly or indirectly affecting the action principles of primary metabolism and secondary metabolism of aerobes, firstly constructing an Escherichia coli expression plasmid pQEV, expressing the VHb with biological activity, and further constructing the saccharopolyspora erythraea expression plasmid (pBlueV). In order to realize high-density culture of cells and high-yield fermentation of metabolic products under oxygen-limited conditions, the invention provides an important pathway for homologous recombination of vgb gene elements and saccharopolyspora erythraea chromosome genomes.

The invention provides a method for optimizing a fermentation medium by improving rapamycin production by using metabolic profiling analysis, which researches dynamic changes and differences of bacteria intracellular metabolites under the culture conditions of initial fermentation medium and optimized fermentation medium by using the methods of dynamic detection and metabolic profiling analysis. The analysis result of partial least squares indicates that the bacteria primary metabolite profile and the rapamycin production have correlation and a further dynamic adding measure is provided to the medium by analyzing the dynamic changes of the key compounds combining related processes. Due to the research of S.hygroscopicus U1-6E7 fermentation medium, the rapamycin production is improved from 196mg / L to 307mg / L and is improved by 56.6%, which indicates that rational guidance of optimization of fermentation mediums by dynamic detection combining the metabolite profile analysis method is capable of successfully improving fermentation level of antibiotic and providing a new idea for optimization of fermentation mediums.

The invention provides a production process for synthesizing macrolide antibiotics special for poultry and livestock through fermentation of acetylisovaleryltylosin tartrate. The production process for synthesizing macrolide antibiotics special for poultry and livestock through fermentation of acetylisovaleryltylosin tartrate comprises a fermentation step, and specifically comprises the followingsteps: A, carrying out primary metabolism thallus culture, namely, carrying out culture so that hyphae grow; and B, carrying out secondary metabolism rhzomorph culture, namely, supplementing the substrate, namely, tylosin solution, adjusting the temperature and carrying out fermentation at 27-29 DEG C, so that the macrolide antibiotics special for poultry and livestock are generated. For the production process for synthesizing macrolide antibiotics special for poultry and livestock through fermentation of acetylisovaleryltylosin tartrate, the content of the component for fermenting the macrolide antibiotics special for poultry and livestock is relatively high, the content of the final component can achieve 80% or above, 3-5 percentage points are improved compared with the prior art, and thus a foundation is laid for the promotion of the quality of the product.

The invention provides honey, lemon and passion fruit tea containing fruit and vegetable enzymes and a preparation method of the tea. Various fruits and vegetables such as sword bean, wild jujube, wrinkled gianthyssop herb, kiwi fruit, litchi, loofah, cane shoots and apple juice are taken as raw materials of the fruit and vegetable enzymes, especially medicinal and edible traditional Chinese medicines including the sword bean, the wild jujube and the wrinkled gianthyssop herb are added, and fermentation is performed on the basis of the materials as a substrate. On the basis of a conventional fermentation strain, Neurospora sitophila, Fomitopsis nigra and Leuconostoc mesenteroides are adopted for batch fermentation, so that metabolic reaction of carbohydrates, protein, lipid and other substances in the raw materials of the fruits and vegetables can be promoted, primary metabolites and secondary metabolites are massively produced and accumulated, various beneficial components such as organic acids, oligosaccharides, sugar alcohol, enzymes, oligomers, polyphenols and the like are generated, and particularly, ester flavor components and amino acids in the fruit and vegetable enzymes are increased substantially.

The invention relates to a tetracycline fermentation feeding medium and a feeding method. According to different demands of thalli for available nitrogen sources and phosphorus amount at different growth and metabolism stages, the amount of available nitrogen sources and phosphorus in the sugar water fed to thalli at different growth stages is improved so as to reach the purpose of improving the tetracycline fermentation level. Experiments prove that, feeding of low-phosphorous sugar water when fermentation is carried out to 60-100h can effectively control the primary metabolism rate, reduce the dissolved oxygen demand, and at the same time is beneficial to formation of secondary metabolites, and the tetracycline yield is significantly increased. The method provided by the invention is simple and practicable, and has reference significance to fermentation controlled by similar ways.

The invention relates to a preparation method of in-bag fermented pickled peppers, and belongs to the fields of biotechnology and foods. The preparation method of the in-bag fermented pickled pepperscomprises the following steps: 1) performing pretreatment on raw materials; 2) performing sterilizing and washing on the pretreated materials; 3) blending a fermentation solution; 4) packaging the treated materials and the fermentation solution with bags, and carrying out vacuuming and sealing; and 5) carrying out fermentation. By adding direct-using lactic acid bacterium powder and primary metabolites of lactobacillus plantarum 360, as well as controlling parameters and indexes during preparation processes, the preparation method of the in-bag fermented pickled peppers is capable of effectively avoiding the problem of gas-producing deterioration caused by reproduction of microorganism heterofermentation at a large amount; so that, after being packaged with bags, color, luster, flavor andcrispness of the in-bag fermented pickled peppers can be maintained within shelf lives of the peppers without production of pickling wastewater. Moreover, the in-bag fermented pickled peppers can be directly sold after completion of fermentation without secondary processing; and thus, clean, standardized and emission-free processing of fermented peppers is realized. Thus, the preparation method ofthe in-bag fermented pickled peppers is of relatively high social and economic benefits for realizing large-scale cross-regional production and promoting development of the pickled vegetable industry.

The present invention provides a method for overexpressing the zwf2 gene to increase the yield of daptomycin and its application, and clones a key enzyme 6-phosphate glucose dehydrogenase gene (zwf2) in the primary metabolic pentose phosphate pathway, using Escherichia coli-Streptomyces Shuttle plasmid expression vector construction of recombinant plasmids. The recombinant plasmid was introduced into Escherichia coli ET12567, ET12567 was co-cultured with Streptomyces roseospora, and Streptomyces roseospora was transformed by the combined transfer method, and then the transformants were screened by apramycin resistance, and the genetically engineered bacteria were further verified by PCR , thereby constructing the genetically engineered strain HP-2. The Streptomyces roseospora genetically engineered bacterium HP-2 constructed by the present invention has an 18% higher output than the starting strain, and the output of daptomycin is as high as 700mg / L, which can be directly used in the industrialized production of daptomycin, which can improve Yield reduces cost.

Belonging to the field of biotechnology and food, the invention relates to a preparation method of fermented capsicum sections in a bag. The method includes the steps of: 1) pretreatment, 2) disinfection and cleaning, 3) slitting, 4) preparation of a fermentation solution, 5) bagging, vacuum pumping and sealing of the treated raw materials and fermented solution, and 6) fermentation. Through adding of direct vat set lactobacillus powder, lactobacillus plantarum 360 primary metabolite and control of all parameters and indexes in the preparation process, the product gas deterioration problem caused by mass propagation of heterofermentation microorganisms can be effectively avoided, the color, flavor and brittleness of the fermented capsicum sections in the bag within the shelf life can be maintained, no pickling wastewater is generated, and the product can be sold directly at the end of fermentation without secondary processing, thus realizing clean, standardized and zero-emission processing and preparation of fermented capsicum sections. The method can realize cross-regional large-scale production and promote the development of the pickle industry, and has good social and economic benefits.

The present invention relates to a method for mass production of other primary metabolites by inhibiting a specific metabolite of metabolism in microorganisms, a transformant for mass production of other primary metabolites plasmid clone by modifying a specific gene relating to the metabolism, and a method for preparation thereof. The primary metabolites can contain lactate, succinate, or alcohol as ethanol, wherein each has a high industrial applicability as an environmental friendly plasmid clone biochemical material.

The present invention relates to a method for manufacturing an organic fertilizer prepared using antagonistic microorganisms, fermentative microorganisms, synthetic microorganisms, and organic raw materials, and to an organic fertilizer manufactured by the manufacturing method. The present invention has the technical feature wherein a powder-type organic raw material characterized by being produced via a contaminant removal step, a sterilization step, a microorganism culture medium preparation step, and a microorganism additive solution preparation step are subjected to a mixing step, a fermentation step, and a drying step. The present invention is effective for soil improvement, crop growth, and disease and pest control. In addition, fertilizer produced through the technical solution contains primary metabolites, secondary metabolites, conjugated enzymes, antibiotics, bioactive substances and inducers, etc., and thus not only does not contaminate the soil, but is also effective for ecosystem restoration, and for increasing profits through the production of safe organic crops.

The invention provides a preparation method of in-bag fermented pickled baby Chinese cabbages, belongs to the field of biotechnology and food and aims to solve the problems that the production cost offermented pickled baby Chinese cabbages is high, the discharge of high-salt and high-acid wastewater is high, color and flavor are instable, fermentation is incontrollable and large-scale standard production cannot be realized. Through control of raw material pretreatment, disinfection and cleaning, pickling and dehydration, mixing, fermentation, fermentation and other steps, particularly controlof addition of direct addition type lactic acid bacteria powder and lactobacillus plantarum 360 primary metabolite products and parameters and indexes in the preparation process, the produced gas deterioration problem caused by mass propagation of heterofermentation microorganisms can be effective solved, the color, flavor, crispness and fullness of the in-bag fermented pickled baby Chinese cabbages in the shelf life can be kept, no wastewater is produced, the in-bag fermented pickled baby Chinese cabbages can be sold directly after fermentation without secondary processing, and clean, standard and zero-emission processing preparation of pickled baby Chinese cabbages is realized, and the method has better social and economic benefits.

The invention discloses a processing method of scutellaria baicalensis, and belongs to the technical field of processing of medicinal materials. The method comprises the following steps: firstly, cleaning collected fresh roots of scutellaria baicalensis; simultaneously heating the fresh roots, and carrying out moisturizing treatment on the fresh roots; keeping the humidity of the fresh roots at 60%-100%, maintaining the state for 1-3 days, and stopping moisturizing of the fresh roots. According to the processing method, the quality of the medicinal materials is improved; the primary metabolism level of the scutellaria baicalensis is improved; the high-yield potential of the medicinal materials is fully excavated; the components are purely natural; the processing technique is designed according to the influence on the quality of the medicinal materials caused by adverse natural environment; the biosynthesis is promoted by the natural process of scutellaria baicalensis biosynthesis, and is different from a biotransformation technology; no reagent is needed to promote high expression of the secondary metabolism pathway; the components contained by the medicinal material of the scutellaria baicalensis are purely natural; and no ingredient with toxic and side effects is generated.

The invention discloses a method for making bagged fermented preserved pickled vegetables, belongs to the fields of biological techniques and foods and aims to solve the problems that conventional fermented preserved pickled vegetables are high in production cost, large in salt and waste acid wastewater emission, instable in color and flavor, uncontrollable in fermentation, hard in on-scale standard production, and the like. By adopting the method, steps of raw material pretreatment, sterilization and cleaning, dehydration, mixing, packaging, fermentation and the like are controlled, particularly directly added lactic acid bacterium powder and primary metabolites thereof are added, and parameters and indexes in the making process are controlled, so that the problem of gas generation deterioration caused by massive propagation of heterofermentation microorganisms can be effectively avoided, the color, the flavor, the crispiness and the fullness of fermented preserved pickled vegetablesin bags within the expiration date can be maintained, no wastewater is generated, the vegetables can be directly sold after fermentation is completed, no secondary processing is needed, and cleaning and standard processing and making of fermented preserved pickled vegetables are achieved.

The invention relates to a method for controlling fermentation of macrolide antibiotics, and solves the problems that the fermentation process of a traditional method is not easy to control, energy consumption is high, and the yield of a target product is low. The method comprises the following steps: controlling the culture temperature at 27.0-30.0 DEG C according to normal requirements in the primary metabolism stage of macrolide antibiotic producing bacteria, and rapidly growing hypha; and reducing the fermentation culture temperature to 18.0-27.0 DEG C after the logarithmic growth phase ofthe macrolide antibiotic producing bacteria is completed, and promoting mass synthesis of the target product, wherein the macrolide antibiotic is an antibiotic produced by fermentation by adopting aPKS biosynthesis pathway and a homologue of the antibiotic. The method has a simple and easy process, is easy to operate, and is very suitable for commercial production.

The invention discloses a preparation method for a pickled green vegetable fermented in bag, belonging to the fields of biotechnology and food. Conventional preparation methods for fermented pickled green vegetables have the problems of high production cost, great emission of high-salt high-acid waste water, unstable flavor, uncontrollable fermentation, difficulty in realizing large-scale standardproduction and the like; directed at the problems, the preparation method of invention performs control on the steps of raw material pretreatment, disinfection and cleaning, dehydration, condiment blending, packaging, fermentation and the like, especially conducts addition of ready-to-use lactic acid bacteria powder and a Lactobacillus plantarum 360 primary metabolite product and controlling of each parameter and each index in the process of preparation, so the preparation method can avoid the problem of deterioration due to gas generation by substantially propagated heterofermentation microbes, maintains the color, flavor and plumpness of the pickled green vegetable fermented in the bag after bagging, and does not produce waste water; and the produced pickled green vegetable fermented inthe bag can be directly sold after completion of fermentation and does not need secondary processing, so clean and standard processing and preparation of the fermented pickled green vegetable is realized.

The invention discloses a mass spectrometry method for high-throughput detection of primary metabolites in corn pollen. A common corn pollen metabolome extracting solution is firstly quickly analyzedby using a gas chromatography-mass spectrometry technology to obtain a comprehensive metabolic profile spectrum. 67 primary metabolites contained in the pollen are systematically identified for the first time. Then, a target metabolome analysis method is established for 67 primary metabolites, and quantitative analysis can be conducted on the corn pollen primary metabolome at a time. The method comprises the steps of sample pretreatment, chromatography-mass spectrometry separation, metabolite result identification and metabolite quantitative analysis. The method has the advantages of good repeatability, high stability, rapidness and reliability, and is suitable for primary metabolome analysis of corn pollen and other crop pollen samples.

The present invention pertains to a homogeneous microorganism extract using useful and functional microorganisms and a method for producing the same, and particularly to: a homogeneous microorganism extract which is obtained by homogenizing and extracting a complex enzyme, various inducing substances and the like by using useful microorganisms and functional microorganisms that are isolated from soil for the purpose of soil improvement, crop growth and pest control; and a method for producing said homogeneous microorganism extract. The method is technically characterized by the following: the homogeneous microorganism extract using useful and functional microorganisms and the method for producing the same are characterized in that: microorganisms are homogenized with a complex enzyme (protein, sugars, fat, fiber, synthetic enzymes for acids and alkalis, and decomposition enzymes etc.), primary metabolites, secondary metabolites, antibiotic substances, various active materials, and the like, which are produced through combination culture in which cultured useful microorganisms and functional microorganisms are made and cultured under a symbiotic relationship such that the useful microorganisms and functional microorganisms serve as a substrate and a raw material; then microorganism inducing substances, crop growth inducing substances, and pest resistance inducing substances are extracted from microorganism cells; and the resultant extract is sterilized and concentrated. Therefore, since said extract includes the complex enzyme, various inducing substances, and the like, the functions of soil improvement, crop growth and pest control are achieved. Accordingly, the present invention is advantageous in cost saving and income increase; advantageous in that the types and number of useful microorganisms can be increased by inducing sunflower bacteria that has been already settled, instead of settling the microorganisms on soil and crops as in conventional microorganism formulations; and advantageous in that the extract directly affects crops so that desired effects are relatively quickly expressed and microbial flora is improved.

The invention discloses a processing method of cornus officinalis, and belongs to the technical field of processing of medicinal materials. The method comprises the following steps: firstly, cleaningcollected fresh roots of cornus officinalis; simultaneously heating the fresh roots, and carrying out moisturizing treatment on the fresh roots; keeping the humidity of the fresh roots at 60%-100%, maintaining the state for 1-3 days, and stopping moisturizing of the fresh roots. According to the processing method, the quality of the medicinal materials is improved; the primary metabolism level ofthe cornus officinalis is improved; the high-yield potential of the medicinal materials is fully excavated; the components are purely natural; the processing technique is designed according to the influence on the quality of the medicinal materials caused by adverse natural environment; the biosynthesis is promoted by the natural process of cornus officinalis biosynthesis, and is different from abiotransformation technology, no reagent is needed to promote high expression of the secondary metabolism pathway, the components contained by the medicinal material of the cornus officinalis are purely natural; and no ingredient with toxic and side effects is generated.

The present invention provides a preparation method of fermented pickled fresh gingers in a bag and belongs to the fields of biotechnology and food. Aiming at problems that currently the prepared pickled fresh gingers are high in production cost; discharge of high-salt and high-acid wastewater is large; color, luster and flavor are unstable; fermentation is uncontrollable; large-scale standardizedproduction is difficult to achieve, etc. By controlling steps of raw material pretreating, disinfecting and washing, fermentation solution preparing, bagging, fermenting, etc., especially adding direct-vat set lactic acid bacterium powder and lactobacillus plantarum 360 primary metabolite products and controlling various parameters and indicators in the preparation, the preparation method can effectively avoid produced gas production deterioration due to mass proliferation of heterogeneous fermenting microorganisms, maintains color, luster, flavor and crispness of the fermented pickled freshgingers in the bag after the bagging during the shelf life, and does not produce waste water. Besides, the fermented pickled fresh gingers can be directly sold after the fermentation is completed anddo not need secondary processing. The preparation method realizes the cleaning, standardized, zero-emission processing and preparation of the pickled fresh gingers, and has relatively good social andeconomic benefits.

The invention relates to a method for preparing in-bag fermented chopped chilli and belongs to the fields of biotechnologies and foods. The method comprises the following steps: 1) performing raw material pretreatment; 2) disinfecting and cleaning the raw materials; 3) chopping the raw materials; 4) stirring; 5) bagging, vacuumizing and sealing; and 6) fermenting. By adding ready-to-use lactic acid abcteria dried powder and lactobacillus plantarum 360 primary metabolite products and controlling various parameters and indicators in the preparation process, the deterioration problem of producedgases due to mass propagation of heterogeneous fermentation microorganisms can be effectively solved, the color, flavor and brittleness of the bagged in-bag fermented chopped chilli in the quality guarantee period can be maintained, pickling wastewater is not produced, and the chopped chilli can be directly sold after fermentation completion and does not need to be subjected to secondary processing. Therefore, clean, standard and zero-discharge processing preparation of the fermented chopped chilli is realized, large-scale cross-regional production is realized, the development of the pickle industry is promoted, and the fermented chopped chilli has excellent social and economic benefits.

The invention discloses a preparation method of in-bag fermented pickled Chinese cabbages, belongs to the field of biotechnology and food and aims to solve the problems that the production cost of fermented pickled Chinese cabbages is high, the discharge of high-salt and high-acid wastewater is high, color and flavor are instable, fermentation is incontrollable and large-scale standard productioncannot be realized. Through control of raw material pretreatment, disinfection and cleaning, dehydration, mixing, vacuum bagging, fermentation and other steps, particularly control of addition of direct addition type lactic acid bacteria powder and primary metabolites thereof and parameters and indexes in the preparation process, the produced gas deterioration problem caused by mass propagation ofheterofermentation microorganisms can be effective solved, the color, flavor, crispness and fullness of the in-bag fermented pickled Chinese cabbages in the shelf life can be kept, no wastewater is produced, the in-bag fermented pickled Chinese cabbages can be sold directly after fermentation without secondary processing, and clean and standard processing preparation of fermented pickled Chinesecabbages is realized.

The invention discloses a Coptis chinensis processing method, and belongs to the technical field of medicinal material processing. The method comprises the following steps: washing collected fresh Coptis chinensis roots, heating and moisturizing the fresh roots to keep the humidity of the fresh roots at 60-100%, keeping the state for 1-3 d, and stopping the moisturizing treatment of the fresh roots. The method improves the quality of the medicinal material, enhances the primary metabolism level of the Coptis chinensis and fully exploits the high yield potential of the medicinal material. The processing method is designed according to the nature that the natural adverse environment affects the quality of the medicinal material, and promotes biosynthesis according to the natural process of biosynthesis of Coptis chinensis, so the processing method is different from a biotransformation technology, does not need any reagents for promoting the high expression of a secondary metabolism approach, can ensure that all components contained in the Coptis chinensis medicinal material purely natural, and does not generate components with possible toxic and side effects, thereby the components of the obtained processed medicinal material are purely natural.