The invention discloses a method for detecting the concentration of ticagrelor and its active metabolites in
human plasma, which comprises the following steps: (1) preparation of a standard working solution; (2)
sample processing; (3) preparation of a
standard curve; 4) Quantitative analysis: take the
test sample and process it according to the method of step (2), and calculate according to the
standard curve equation obtained in step (3), to obtain the concentration of
ticagrelor and its
active metabolite in the sample to be tested. The present invention uses high-performance liquid
chromatography (HPLC) to measure the concentration of
ticagrelor and its active metabolites in
human plasma, and accurately quantifies the concentration of ticagrelor and its active metabolites in
human plasma. Reasonable selection of methods, mobile phases,
mass spectrometry conditions, etc., optimization of experimental conditions such as flow rate, mobile
phase ratio, and chromatographic conditions, etc. After a series of methodological verifications, the entire quantitative detection method is practical, easy to operate, high in
repeatability, and good in accuracy , strong
operability, and can be directly applied to the detection and analysis of
plasma samples of clinical patients and related
pharmacokinetic analysis.