37 results about "Nonadherent cell" patented technology

The present invention provides a microorganism culture array, including one or more microflow cell culture array units, the microflow cell culture array unit includes a main channel and a plurality of cell culture units connected with the main channel, each culture unit includes a diffusion channel and a cell culture room, the cell culture room is connected with the main channel. The microorganism culture array of the invention not only realizes isolation of single-cell, but also makes the cell locate in zero flow state when the culture liquid or other reagent is changed, bringing greatly convenience for the cell culture and observation especially the culture and observation of non-adherent cells, greatly fit for research work of high throughput screen based on the cell phenotype.

The invention discloses a method used for separation and culture of poultry endothelial progenitor cells (EPCs). The method comprises following steps: peripheral blood is collected and separated so as to obtain mononuclear cells; the mononuclear cells are cultured in a culture solution; and a mixture of adherent cells and non-adherent cells is used for culture. It is possible to obtain a large amount of EPCs by separation and culture of poultry peripheral blood using the method.

A method of enriching stem or progenitor cells that includes growing a heterogeneous cell sample comprising stem and/or progenitor cells on a first substrate that is hydrophobic and has an elastic modulus less than about 100 MPa; recovering the heterogeneous cell sample from the first substrate; growing the recovered heterogeneous cell sample on a second substrate that is hydrophilic and has an elastic modulus higher than the elastic modulus of the first substrate to produce a subpopulation of nonadherent cells and a subpopulation of adherent cells; and recovering the nonadherent cell subpopulation, which is enriched for stem and/or progenitor cells. The invention also relates to a method of determining the clonogenic potential of a cell, such as a cancer stem cell enriched using the enrichment method described herein.

The invention relates to methods of obtaining mesenchymal stem cells and is to provide a method of high-effectively obtaining mesenchymal stem cells from compact bone of mice. The method of high-effectively obtaining mesenchymal stem cells of mice includes the steps of: separating mesenchymal stem cells of mice, performing primary culture, and performing amplification culture to the mesenchymal stem cells of mice. The method greatly reduces the time of cell culture and can greatly increase quantity of produced cells, and is less in pollution of non-MSCs adherent cells. The invention, for the first time, discloses a multi-time bone sheet adhesion culture method, which can increase the yield of cells by 3-5 times. Through systematic researching on biological characteristics, such as growth status, surface markers, multidirectional differentiative potential and the like, of the MSCs from the compact bone of mice, it is proved that after 10 times of amplification culture in vitro, the cells still have strong characters of stem cells and can satisfy demands in experiment and researching. The method can quickly and high-effectively produce the stem cells. The obtained stem cells has high proliferation and growth speed and high differentiative potential.

the invention discloses a separating method of ox marrow mesenchyma stem cell, which comprises the following steps: adding ox marrow single-cell suspension liquid in the Percoll cell separating liquid with density at 1.082 to separate; obtaining foggy single-core cell at liquid intersecting layer; inoculating in the plastic container; removing non-adhesive wall cell to obtain the product with separating purifying efficiency over 98%.

Disclosed is a device for cell culture, the device includes a first chamber having an area for containing cells, such as isolated cells, for growth; a second chamber for containing culture medium; a semi permeable membrane forming at least part of a divider between the first chamber and the second chamber; wherein the area for containing cells for growth is adjustable via a moveable wall disposed within the first chamber. The device is especially suited for culturing of semi-adherent and non-adherent cells.

The invention discloses use of mesenchymal stem cells of dental pulp in obtaining of an artificial spinal cord and a preparation method for the artificial spinal cord. The preparation method consists of the following steps: (1) acquiring healthy deciduous teeth removed due to deformity correcting, digesting the healthy deciduous teeth with type 1 collagenase, inoculating a culture flask with the digested substance, carrying out washing to discard nonadherent cells, re-suspending adherent cells, then, carrying out culture, so as to obtain the mesenchymal stem cells of the dental pulp; (2) culturing the mesenchymal stem cells of the dental pulp with an alpha-MEM culture medium, so as to propagate the mesenchymal stem cells of the dental pulp; and (3) selecting third-generation dental-pulp mesenchymal stem cells, culturing the dental-pulp mesenchymal stem cells with an alpha-MEM culture medium containing vitamin C when the cell fusion rate is 90%so as to obtain a white membrane-shaped structure, and carrying out in-vitro molding, thereby finally obtaining the artificial spinal cord with a three-dimensional structure. The artificial spinal cord obtained by the preparation method can be used for inhibiting cicatrization and inflammation and promoting axon regeneration; and among rats suffering from complete spinal injury, Spinor therapy causes remarkable movement improvement, perception recovery and faster urination reflection recovery.

The process of separating mesenchymal stem/ancestral cell from bone parenchyma includes the following steps: 1) digesting bone sclerite with collagenase; and 2) culturing the digested bone sclerite in culture medium, eliminating non-adherent cell and obtaining the adherent cell as the mesenchymal stem/ancestral cell. The process of the present invention is simple and practical, and the obtained mesenchymal stem/ancestral cell has high purity, no residual blood cell and endothelial cell and good differentiating capacity, and can differentiate to osteoplast, chondroblast, lipoblast, etc. The present invention establishes stable and practical mouse MSPC cell purifying culture technological system and lays foundation for the MSPC cell research.

The present invention relates to an apparatus for analyzing cells in real-time which incorporates a wavelength-tunable light source/infrared ray (IR) sensor and can be used to observe and analyze the IR-related characteristics of adherent cells or non-adherent cells. The Apparatus for analyzing cells in real-time in accordance with the present invention can be used to quantify specific materials in a cell and measure the metabolism of a cell. In addition, the apparatus for analyzing cells in real-time in accordance with the present invention can be configured to have a visible light microscope coupled thereto, and in this configuration, it can be used to locate a cell of interest.

The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

It is not understood what causes or influences pattern formation in cells during the development of an organism. When animal/human cells are cultured in a Petri dish the adherent cells attach to the bottom of the dish, whereas the non-adherent cells float in the growing medium. Currently there are no specialized dishes for culturing non-adherent cells. We now show that non-adherent cells could be induced to form distinct patterns when cultured in an etched plastic dish (Biosimulator). The non-adherent cells showed polarity when cultured in the etched plate. The polarity/pattern formation could be reversed with inhibitors specific for adhesion proteins. The phenomenon of pattern formation by non-adherent cells has wide applications in cell and developmental biology, diagnostics, microbiome research, biofluidics, drug discovery, industrial production of biological products, and also in biotechnology and bioengineering.

The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells, e.g. T cells.