Method used for separation and culture of poultry endothelial progenitor cells

Abstract

The invention discloses a method used for separation and culture of poultry endothelial progenitor cells (EPCs). The method comprises following steps: peripheral blood is collected and separated so as to obtain mononuclear cells; the mononuclear cells are cultured in a culture solution; and a mixture of adherent cells and non-adherent cells is used for culture. It is possible to obtain a large amount of EPCs by separation and culture of poultry peripheral blood using the method.

Description

technical field [0001] The invention relates to the field of poultry tissue and cell culture engineering, in particular to a method for isolating and culturing poultry endothelial progenitor cells. Background technique [0002] Endothelial progenitor cells (EPCs) are a type of precursor cells that can differentiate into vascular endothelial cells. They not only participate in embryonic angiogenesis, but also participate in postnatal angiogenesis and repair after vascular endothelial injury. When the vascular endothelium is damaged, EPCs in the systemic circulation migrate to specific sites for differentiation and proliferation under the action of various factors, and promote vascular repair. The vascular repair and directional migration of EPCs have shown broad application prospects in the field of cardiovascular disease treatment and gene targeting therapy, and are one of the hotspots in the field of biomedical research. [0003] At present, poultry has become an important...

AI Technical Summary

Problems solved by technology

However, after many explorations, we found that it is difficult to obtain poultry EPCs by simply culturing adherent cells or non-adherent cells, indicating that the method of isolating and culturing mammalian EPCs is not suitable for isolating and culturing poultry EPCs

Images