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Method for separating mesenchy mall stem/ancestral cells for bone parenchyma

A progenitor cell, substantial technology, applied in the field of separation of stem cells, can solve the problem of remaining blood cells, not stem cells, etc., and achieve the effects of good differentiation performance, high purity and simple operation

Inactive Publication Date: 2007-01-03
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it can be considered that not only blood cells remain in the adherent cell population obtained by the above method, but also some cells may not be true stem cells.

Method used

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  • Method for separating mesenchy mall stem/ancestral cells for bone parenchyma
  • Method for separating mesenchy mall stem/ancestral cells for bone parenchyma
  • Method for separating mesenchy mall stem/ancestral cells for bone parenchyma

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Embodiment 1

[0015] Example 1. Isolation and Identification of Mouse Mesenchymal Stem / Progenitor Cells

[0016] 1. Isolation of mouse mesenchymal stem / progenitor cells (MSPCs)

[0017] 1. Experimental materials

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Abstract

The process of separating mesenchymal stem / ancestral cell from bone parenchyma includes the following steps: 1) digesting bone sclerite with collagenase; and 2) culturing the digested bone sclerite in culture medium, eliminating non-adherent cell and obtaining the adherent cell as the mesenchymal stem / ancestral cell. The process of the present invention is simple and practical, and the obtained mesenchymal stem / ancestral cell has high purity, no residual blood cell and endothelial cell and good differentiating capacity, and can differentiate to osteoplast, chondroblast, lipoblast, etc. The present invention establishes stable and practical mouse MSPC cell purifying culture technological system and lays foundation for the MSPC cell research.

Description

technical field [0001] The invention relates to a method for isolating stem cells, in particular to a method for isolating mesenchymal stem / progenitor cells from bone parenchyma. Background technique [0002] Mesenchymal stem cells (MSCs) are non-hematopoietic stem cells in the bone marrow, which have the ability to differentiate into osteoblasts, chondrocytes, and adipocytes, and have immune regulation and hematopoietic support functions. Therefore, MSCs are not only important seed cells in the construction of tissue-engineered bone and cartilage, but also have broad application prospects in hematopoietic cell transplantation because they can promote hematopoietic reconstruction and inhibit graft-versus-host reaction. MSC has the property of adherent growth in vitro. Using the adherent property of cells, people have successfully isolated MSC from human, dog, rabbit, rat, chicken, sheep, goat, pig and bovine bone marrow. [0003] When other animal bone marrow cells such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/06
Inventor 李秀森李红郭子宽于晓妉毛宁
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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