Chip for evaluating mobile biological living body pharmacokinetics and real-time detection system

Abstract

The invention discloses a chip for evaluating mobile biological living body pharmacokinetics and a real-time detection system. The chip comprises a chip main body, at least one fluid flow passage arranged in the chip main body, a fluid outlet opened in the chip main body, and a passageway main body connecting a fluid inlet with the fluid outlet, wherein each fluid flow passage is provided with thefluid inlet which is opened in the chip main body; the passageway main body is internally provided with at least one referential chamber and at least one sample chamber, the sample chamber is provided with a liquid permeating protective screen which is vertically arranged in the passageway main body, and is arranged in the passageway main body through the liquid permeating protective screen in afluid communication way, the referential chamber and the sample chamber are separated from each other by a certain distance in space, and the referential chamber has the same shape and size as those of the sample chamber. The chip disclosed by the invention can be used for pharmacokinetics detection on fixed samples and non-fixed samples such as non-adherent cells, microorganisms and tiny organisms.

Description

technical field [0001] The invention relates to the technical field of biodetection chips, in particular to a chip that can be used for real-time dynamic detection, especially for pharmacokinetic research of non-fixed cells, tissues, microorganisms or tiny organisms. Background technique [0002] Pharmacokinetics studies the changes and laws of drugs under the influence of the body. Pharmacokinetics studies the in vivo process of drugs (including absorption, distribution, metabolism and excretion), and uses mathematical principles and methods to explain the dynamic laws of drugs in the body. However, the real-time uptake and metabolic kinetics of specific drug molecules by cells is crucial for understanding cell metabolism, the physiological state of related proteins and enzymes, the effects of drugs (such as agonists / inhibitors), the interpretation of molecular imaging results, and the identification of medical imaging probes. Development, etc. are also of great significan...

AI Technical Summary

Problems solved by technology

[0006] However, the core of these two technologies is to achieve the purpose of separating detection signals by fixing cells in a specific area, so they are limited to certain specific detection objects, such as adherent cells, and cannot detect non-fixed detection objects. (such as non-adherent growth cells, microorganisms, isolated tissue pieces, microscopic organisms, etc.) for detection

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