Microflow cell culture array and application thereof

Abstract

The present invention provides a microorganism culture array, including one or more microflow cell culture array units, the microflow cell culture array unit includes a main channel and a plurality of cell culture units connected with the main channel, each culture unit includes a diffusion channel and a cell culture room, the cell culture room is connected with the main channel. The microorganism culture array of the invention not only realizes isolation of single-cell, but also makes the cell locate in zero flow state when the culture liquid or other reagent is changed, bringing greatly convenience for the cell culture and observation especially the culture and observation of non-adherent cells, greatly fit for research work of high throughput screen based on the cell phenotype.

Description

technical field [0001] The invention belongs to the field of microfluidic technology and cell biology, and in particular relates to a microfluidic chip device suitable for culturing cells or microorganisms in a microenvironment. Background technique [0002] Microfluidic chip (Microfluidic chip) originally originated in the field of analytical chemistry. It is a kind of micro-channel network structure and other functional units made on a substrate of several square centimeters by using fine processing technology to realize the preparation of micro-sample, processing A fast, efficient and low-consumption micro-analysis experimental device integrating sample, reaction, separation and detection. [0003] The application of microfluidic chip technology in cell culture and its biochemical analysis has attracted widespread attention, such as cell manipulation, expression of green fluorescent protein, gene transfection, cell activity testing, cell separation, measurement of intrace...

AI Technical Summary

Problems solved by technology

Although these methods are useful, they require special surface treatment and require complex microfabrication processes or manipulations, making cell arrays in microfluidic systems not widely available to biologists.

Moreover, in these methods, the supply of nutrients to cells is mainly achieved through the flow of fluid around the cells, and the cells will be under a certain flow rate, which will bring inconvenience to the cultivation and observation of some non-adherent cells.

At the same time, they can't observe the growth of cells well for a long time.

[0005] Generally speaking, research on microfluidics has just started in the world. On the one hand, the research is relatively preliminary and the experiments are not rich enough;

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