Method used for separation and culture of poultry endothelial progenitor cells

Abstract

The invention discloses a method for isolating and culturing poultry endothelial progenitor cells, which is characterized by comprising the following steps: collecting peripheral blood, separating and collecting mononuclear cells, adding culture medium for cell culture, using adherent cells and non-adherent cells The mixture is cultivated. According to the method for isolating and culturing poultry endothelial progenitor cells of the present invention, a large amount of EPCs can be isolated and cultured from poultry peripheral blood.

Description

Technical field [0001] The invention involves the field of poultry tissue and cell culture engineering, especially a method of separating poultry endothelial ancestral cells. Background technique [0002] ENDOTHELILILILILILILILILILILITOR CELLS (EPCS) is a type of pre -body cells with differentiated vascular endothelial cells. It not only participates in the occurrence of blood vessels during the embryo, but also participates in the repair process after new blood vessels and vascular endothelial damage.When the vascular endothelial is damaged, the EPCS in the body cycle migrates to a specific site under the action of multiple factors for differentiation and proliferation to promote blood vessel repair.EPCS's vascular repair and directional migration effects have shown extensive application prospects in the field of cardiovascular disease therapy and the field of genetic targeted therapy, which is one of the hot spots in the field of biomedicine. [0003] At present, poultry has be...

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Problems solved by technology

However, after many explorations, we found that it is difficult to obtain poultry EPCs by simply culturing adherent cells or non-adherent cells, indicating that the method of isolating and culturing mammalian EPCs is not suitable for isolating and culturing poultry EPCs

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