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Method used for separation and culture of poultry endothelial progenitor cells

A technology for the isolation and cultivation of endothelial progenitor cells, which is applied in the field of poultry tissue and cell culture engineering, and can solve problems such as the difficulty in obtaining poultry EPCs and the inapplicability of separating and culturing poultry EPCs

Inactive Publication Date: 2015-06-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after many explorations, we found that it is difficult to obtain poultry EPCs by simply culturing adherent cells or non-adherent cells, indicating that the method of isolating and culturing mammalian EPCs is not suitable for isolating and culturing poultry EPCs

Method used

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  • Method used for separation and culture of poultry endothelial progenitor cells
  • Method used for separation and culture of poultry endothelial progenitor cells
  • Method used for separation and culture of poultry endothelial progenitor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1 material and method

[0019] 1.1 Experimental animal

[0020] AA meat chicken on the 20th.

[0021] Treatment of cell culture board

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Abstract

The invention discloses a method for isolating and culturing poultry endothelial progenitor cells, which is characterized by comprising the following steps: collecting peripheral blood, separating and collecting mononuclear cells, adding culture medium for cell culture, using adherent cells and non-adherent cells The mixture is cultivated. According to the method for isolating and culturing poultry endothelial progenitor cells of the present invention, a large amount of EPCs can be isolated and cultured from poultry peripheral blood.

Description

Technical field [0001] The invention involves the field of poultry tissue and cell culture engineering, especially a method of separating poultry endothelial ancestral cells. Background technique [0002] ENDOTHELILILILILILILILILILILITOR CELLS (EPCS) is a type of pre -body cells with differentiated vascular endothelial cells. It not only participates in the occurrence of blood vessels during the embryo, but also participates in the repair process after new blood vessels and vascular endothelial damage.When the vascular endothelial is damaged, the EPCS in the body cycle migrates to a specific site under the action of multiple factors for differentiation and proliferation to promote blood vessel repair.EPCS's vascular repair and directional migration effects have shown extensive application prospects in the field of cardiovascular disease therapy and the field of genetic targeted therapy, which is one of the hot spots in the field of biomedicine. [0003] At present, poultry has be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071
Inventor 谭勋毕师诚
Owner ZHEJIANG UNIV
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