The invention discloses a method for
rapid detection and authenticity identification of
fatty acid of racy
camellia oil by near
infrared transmission spectroscopy (NITS). The method comprises the steps of: screening high quality varieties with
oil content of 30%-60% from more than 130 camellia seed varieties, wherein in the
camellia oil, the content of oleinic acid is 65%-85%, the content of
linoleic acid is 5%-20%, the content of
palmitic acid is 3%-15%, and the content of
stearic acid is 1%-5%; establishing a near
infrared transmission spectroscopy (NITS) model of the main fatty acids (oleinic acid,
linoleic acid,
palmitic acid,
stearic acid and total
unsaturated fatty acid) with a partial
least squares (PLS) method by adopting the Fourier near
infrared transmission spectroscopy (NITS), wherein the determination coefficients of the correction models of the oleinic acid, the
linoleic acid and the
palmitic acid respectively are 0.93446, 0.96538 and 0.88789, and the determination coefficients of
cross validation respectively are 0.91987, 0.95755 and 0.84447; further verifying accuracy and reliability of five NIRS (
Near Infrared Reflectance Spectroscopy) models by external
verification so as to obtain related coefficients of the external
verification, which respectively are 0.9424, 0.9682, 0.8862, 0.6834 and 0.7587; and evaluating doped
camellia oil by establishing the NIRS model of binary
system miscella of the camellia oil. The method is applicable to rapid measurement of the content of main fatty acids of the camellia oil and the doped camellia oil, has the characteristics of simplicity, convenience, rapidness and economy, and is suitable for industrial application.