The invention relates to a
CRISPR detection primer set for MTBC (
Mycobacterium Tuberculosis Complex) and use thereof, and belongs to the technical field of
gene detection. The primer set includes an amplification primer pair and a crRNA; the amplification primer pair is used to amplify a common
conserved sequence of M.
tuberculosis, M. bovis, M. africanum, M. canettii, and M. microti; the crRNA includes an anchor sequence and a guide sequence, wherein the anchor sequence is bound to a cas
protein, and the guide sequence is matched with a targeted
RNA fragment in the common
conserved sequence.Through the primer set based on
CRISPR detection technology, the detection of common conserved sequences of M.
tuberculosis, M. bovis, M. africanum, M. canettii, and M. microti by
CRISPR detection canquickly perform on-site detection of MTBC, and have the advantages of high specificity, high sensitivity and simplicity.