The invention belongs to the technical field of network security, and particularly relates to a moving target defense decision selection method, device and system based on a Markov time game, and themethod comprises the steps: building a moving target attack and defense strategy set; analyzing a moving target defense process based on the Markov time game, and constructing a Markov time game moving target defense model; and carrying out equilibrium solution on the Markov time game moving target defense model, and determining an optimal strategy in the confrontation process of the Markov time game moving target defense model. On the basis of analyzing a moving target attack and defense process, a Markov time game model is constructed, a target criterion function of a moving target defense game is designed based on discount total revenue, and multi-stage attack and defense game quantitative analysis is realized; designing a multi-stage optimal defense strategy selection algorithm based on nonlinear programming multi-stage game equilibrium calculation; further, experimental research results show that the confrontation process can be effectively described, the optimal strategy can be correctly selected, the implementation accuracy of moving target defense can be ensured, and network security management personnel can be helped to make a better decision.