34 results about "Membrane staining" patented technology

The invention relates to a family of cyanine dyes which fluoresce in the far red and near infra red wavelengths of the spectrum and preferably possess lipophilic side chains. The dyes of the invention are soluble in commercially available membrane staining vehicles, are useful as probes for rapidly staining lipophilic structures such as membranes in cells or isolated from cells, and are well retained therein. Methods of using the dyes to detect stained cells both in vivo and in vitro are also disclosed.

Methods and systems for image scoring and analysis are provided. Scored and analyzed images may include digital pathology images. Image scoring and analysis methods may include techniques to identify nuclei and determine membrane staining extent through the use of a priori models. Image scoring and analysis methods may include techniques for membrane intensity determination. Images may be scored based on an extent of membrane staining and membrane intensity.

The invention relates to an image processing method, device and equipment for immunohistochemical PD‑L1 membrane staining pathological sections. The image processing method includes: obtaining the full-field image of the digital slice of the immunohistochemical PD‑L1 (SP263) membrane stained pathological section to be diagnosed; using the region segmentation network to analyze the tumor cells in the full-field image of the digital slice under the magnification of the first field of view. The area is identified and segmented to obtain the tumor cell area probability map of the entire digital slice full-field map; the cells in each digital slice full-field map are identified and segmented, and the cell location network is evaluated using the tumor cell area probability map as a weight matrix. Regional constraints, identifying cell features on the full-field map of digital slices, positioning and classifying various types of cells on the full-field map of digital slices; marking the cell position, cell type and immunohistochemical PD‑ on the full-field map of digital slices L1 (SP263) indicator. This application designs a multi-level feature collaborative diagnosis strategy and uses regional features to constrain cell features to accurately evaluate tumor proportion scores.

The invention discloses an anti-short-wave visible light damage window film, which sequentially comprises a composite refraction coating, an anti-blue light base film, a dyed adhesive layer, an anti-ultraviolet light base film, a pressure-sensitive adhesive layer and a release film from the outside to the inside. The present invention absorbs and blocks short-waves in visible light, especially blue light, cyan and violet light, through the cooperation of double-layered base films, so as to obtain a window film that can prevent short-wave damage of visible light. In addition, the present invention can also adjust the basic tone of the window film after absorbing blue-violet light through the dyed adhesive layer, making it as colorless and transparent as possible, so that the window film can be more easily and widely applied to various occasions.

Disclosed in the present invention is a purine skeleton-based no-wash, aggregation-induced plasma membrane targeted staining reagent, and a preparation method and an application thereof, the present invention using a purine skeleton as a base for a plasma membrane stain, obtaining, by means of reasonable design regulating a fat-soluble end and a hydrophilic end, a purine skeleton-based plasma membrane targeted staining reagent, the reagent being capable of both rapid targeted staining and remaining on a plasma membrane for a relatively long time, benefiting long-term detection. In addition, given that the staining reagent has characteristics of an aggregation-induced compound, emitting light weakly or not emitting light in solution, and exhibiting strong fluorescence when not in solution, causing the stain to further have specific no-wash performance. The preparation method of the present invention has high yield and mild reaction conditions, and the prepared staining reagent has a large Stokes shift and high targeting.

The present invention is concerned with a staining dye for staining a biological cell, comprising dye molecules with a positively charged polar head group for attraction to cell membrane of the cell and for refraining the dye molecules from entering the cytoplasm, and one or more hydrophobic groups for interaction with phospholipids of the cell membrane. The dye molecules are phosphorescent transition metal polypyridine complexes having a metal center and ligands which are non-organic fluorophores.

The invention relates to the detection field, in particular to a digestive tract mucosa stain, a preparation method and an application thereof. The digestive tract mucosa staining agent provided by the present invention includes a first dyeing pigment and a second dyeing pigment; the first dyeing pigment includes a blue pigment; and the second dyeing pigment includes a red pigment. When the digestive tract mucous membrane staining agent provided by the present invention is used for staining, it can form a clear contrast with the color of the mucous membrane itself, excellently display the subtle concave-convex changes of the mucous membrane, and display various raised, flat, sunken lesions and their three-dimensional structures, Strong three-dimensional sense, can clearly identify the details in the digestive tract, identify the boundaries of lesions, and significantly improve the detection rate of lesions. In addition, the digestive tract mucosa stain provided by the present invention is very easy to clean, has less residue after washing, and has a clear surgical field, which has good clinical application value.

The invention belongs to the field of medical technology, and discloses a method for staining the anterior lens capsule in overmature cataract surgery. Take 240 clean-grade male Wistar rats with a body weight of 200-250 g; the animals are randomly divided into a normal control group and an experimental group; The experimental group was dilated with compound tropicamide eye drops three times before operation, anesthetized by intramuscular injection of a mixture of ketamine hydrochloride and chlorpromazine hydrochloride, then made an upper limbal incision, injected methylene blue injection into the anterior chamber, and stayed in the anterior chamber Afterwards, fully flush with compound sodium chloride injection; inject appropriate amount of viscoelastic agent, continuously circular capsulorhexis, observe tissue contrast; continue capsulorhexis after trimming with capsular shears. The present invention does not require additional preparation, can be taken at any time, and has good biocompatibility; it has no drug chemical toxicity to intraocular tissues, such as corneal endothelial cells and visual cells; residual traces can be discharged through normal intraocular absorption or aqueous humor circulation; no Obvious complications.

The present application provides a medical image processing method, device, terminal, and storage medium. The method includes: obtaining a segmented image of blood vessels corresponding to a stained enlarged image of esophageal background mucous membrane; obtaining a segmented image corresponding to a region lacking in blood vessels; Perform image superposition processing on the segmented image of the blood vessel area to obtain the superimposed blood vessel segmentation image; obtain the background mucosa color representation image corresponding to the esophagus background mucosa staining enlarged image; obtain the background mucosa smoothness representation image corresponding to the esophageal background mucosa staining enlargement image; and superimpose the blood vessel segmentation Image, background mucosal color representation image and background mucosa smoothness representation image are processed by channel fusion to obtain esophageal background mucosal staining enlarged composite image; based on esophageal background mucosal staining enlarged composite image, determine the esophageal markers corresponding to the esophageal background mucosal staining zoom-in image Infiltration depth. Improved accuracy of depth of invasion determination for esophageal markers.