34 results about "Membrane staining" patented technology

The invention relates to an image processing method, device and equipment for immunohistochemical PD‑L1 membrane staining pathological sections. The image processing method includes: obtaining the full-field image of the digital slice of the immunohistochemical PD‑L1 (SP263) membrane stained pathological section to be diagnosed; using the region segmentation network to analyze the tumor cells in the full-field image of the digital slice under the magnification of the first field of view. The area is identified and segmented to obtain the tumor cell area probability map of the entire digital slice full-field map; the cells in each digital slice full-field map are identified and segmented, and the cell location network is evaluated using the tumor cell area probability map as a weight matrix. Regional constraints, identifying cell features on the full-field map of digital slices, positioning and classifying various types of cells on the full-field map of digital slices; marking the cell position, cell type and immunohistochemical PD‑ on the full-field map of digital slices L1 (SP263) indicator. This application designs a multi-level feature collaborative diagnosis strategy and uses regional features to constrain cell features to accurately evaluate tumor proportion scores.

Disclosed in the present invention is a purine skeleton-based no-wash, aggregation-induced plasma membrane targeted staining reagent, and a preparation method and an application thereof, the present invention using a purine skeleton as a base for a plasma membrane stain, obtaining, by means of reasonable design regulating a fat-soluble end and a hydrophilic end, a purine skeleton-based plasma membrane targeted staining reagent, the reagent being capable of both rapid targeted staining and remaining on a plasma membrane for a relatively long time, benefiting long-term detection. In addition, given that the staining reagent has characteristics of an aggregation-induced compound, emitting light weakly or not emitting light in solution, and exhibiting strong fluorescence when not in solution, causing the stain to further have specific no-wash performance. The preparation method of the present invention has high yield and mild reaction conditions, and the prepared staining reagent has a large Stokes shift and high targeting.

The present invention is concerned with a staining dye for staining a biological cell, comprising dye molecules with a positively charged polar head group for attraction to cell membrane of the cell and for refraining the dye molecules from entering the cytoplasm, and one or more hydrophobic groups for interaction with phospholipids of the cell membrane. The dye molecules are phosphorescent transition metal polypyridine complexes having a metal center and ligands which are non-organic fluorophores.

The invention belongs to the field of medical technology, and discloses a method for staining the anterior lens capsule in overmature cataract surgery. Take 240 clean-grade male Wistar rats with a body weight of 200-250 g; the animals are randomly divided into a normal control group and an experimental group; The experimental group was dilated with compound tropicamide eye drops three times before operation, anesthetized by intramuscular injection of a mixture of ketamine hydrochloride and chlorpromazine hydrochloride, then made an upper limbal incision, injected methylene blue injection into the anterior chamber, and stayed in the anterior chamber Afterwards, fully flush with compound sodium chloride injection; inject appropriate amount of viscoelastic agent, continuously circular capsulorhexis, observe tissue contrast; continue capsulorhexis after trimming with capsular shears. The present invention does not require additional preparation, can be taken at any time, and has good biocompatibility; it has no drug chemical toxicity to intraocular tissues, such as corneal endothelial cells and visual cells; residual traces can be discharged through normal intraocular absorption or aqueous humor circulation; no Obvious complications.