34 results about "Membrane staining" patented technology

The invention relates to an immunohistochemical PD-L1 membrane staining pathological section image processing method, device and equipment. The image processing method comprises the following steps: acquiring a digital section full-field image of a to-be-diagnosed immunohistochemical PD-L1 (SP263) membrane staining pathological section; adopting a region segmentation network to identify and segment a tumor cell region in the digital slice full-field image under the first visual field multiplying power to obtain a tumor cell region probability graph of the whole digital slice full-field image;identifying and segmenting cells in each digital slice full-field graph, taking the tumor cell region probability graph as a weight matrix to carry out region constraint on the cell positioning network, identifying cell characteristics on the digital slice full-field graph, and positioning and classifying various cells on the digital slice full-field graph; and marking the cell position, the celltype and the immunohistochemical PD-L1 (SP263) index on the full-field diagram of the digital slice. By designing a multi-level feature collaborative diagnosis strategy, the tumor proportion score isaccurately evaluated in a mode of constraining cell features by using regional features.

The invention relates to an immunohistochemical membrane staining section diagnosis method. The method comprises the following steps: S10, receiving a digital section full-field graph of a to-be-diagnosed immunohistochemical membrane staining pathological section; S20, performing quality evaluation on the region of interest on the digital slice full-field image, and performing image restoration onthe region with unqualified quality evaluation to obtain a region of interest with qualified quality evaluation; S30, carrying out cell positioning, classification and segmentation on the regions ofinterest with qualified quality evaluation, marking cell positions and cell types on the regions of interest, and calculating and marking immunohistochemical indexes; and S40, displaying the marked region of interest. The invention further relates to an immunohistochemical membrane staining section diagnosis device and a computer readable medium. Since the context information of the lesion area isof great significance to interpretation of the immunohistochemical membrane staining section, the accuracy of interpretation is ensured by collecting the eight-connected patch image of the immunohistochemical membrane staining pathological section with time sequence for training and testing.

The invention relates to a family of cyanine dyes which fluoresce in the far red and near infra red wavelengths of the spectrum and preferably possess lipophilic side chains. The dyes of the invention are soluble in commercially available membrane staining vehicles, are useful as probes for rapidly staining lipophilic structures such as membranes in cells or isolated from cells, and are well retained therein. Methods of using the dyes to detect stained cells both in vivo and in vitro are also disclosed.

The invention relates to a family of dyes which fluoresce in the UV-VIS, far red and near infrared wavelengths of the spectrum and possess asymmetric lipophilic alkyl chains. The dyes of the invention are soluble in commercially available membrane staining dyes, are useful as probes for rapidly staining lipophilic structures such as membranes in cells or isolated from cells, and are well-retained therein. Methods of utilizing the dyes to detect stained cells both in vivo and in vitro are also disclosed.

The invention relates to an indicarmine mucosa staining agent used for endoscopic staining development, and a preparation method thereof. The indicarmine mucosa staining agent comprises 0.15-0.25wt% of indicarmine and 99.75-99.85wt% of purified water. The display of the microstructure by utilizing the difference of the color obtained after the deposition of the staining agent belongs to a physical effect and has no chemical reactions, the infection focus cannot be observed by using tissue staining, and the staining agent does not react with mucosa tissues. The staining agent is deposited on the mucosal fold, is not absorbed, does not combine with mucus, is discharged from the body through the intestinal tract, has no side effects, has no restricted theoretic use dosage, is easy to flush, and can repeatedly stain until a satisfactory effect is reached.

The invention discloses a lactobacillus rhamnosus R7970 as well as a product and application thereof. The lactobacillus rhamnosus R7970 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.22244. The lactobacillus rhamnosus R7970 is a lactobacillus rhamnosus R7970. Compared with an original strain M9, the lactobacillus rhamnosus R7970 provided by the invention has the advantages that the bacterial colony is obviously increased, the surface is smooth, the edge is neat, the capsular can be obviously observed after the capsular staining, the surface roughness is obviously increased, the smooth degree is obviously reduced, the boundary between the cell wall and the outer capsular is clear, and more capsular polysaccharide is generated; the capsular polysaccharide has higher resistance to the environment, the survival rate of the capsular polysaccharide in intestinal tracts is improved, and the capsular polysaccharide has immunocompetence; in addition, the viscosity of the fermentation liquor can be remarkably increased, and the method is extremely suitable for preparing high-viscosity fermentation products.

The invention provides a plasma membrane fluorescence labeling method of a plant and a plasma membrane protein positioning and dynamic monitoring method, and belongs to the technical field of plasma membrane dyeing. According to the invention, the FM4-64 dye is adopted to dye the plasma membrane structure, and the FM4-64 dye can be embedded in the plasma membrane bimolecular layer and can be dynamically transferred along with the plasma membrane, so that the FM4-64 dye can also be used by subsequent related researches such as endocytosis and transfer of the plasma membrane. And moreover, the fluorescence labeling method disclosed by the invention can be used for carrying out fluorescence labeling on the plasma membrane during genetic transformation or instantaneous transformation, so that the experiment time is saved, and the interference problem of a fluorescence signal does not exist.

The invention relates to a kit for staining esophageal mucosa. A staining solution used in esophageal mucosa staining by the kit is a compound iodine solution, and a neutralizing solution is a vitaminC solution. The staining solution is 20mL of the compound iodine solution and consists of iodine, potassium iodide and distilled water, wherein each milliliter of the compound iodine solution contains 0.02g iodine and 0.04g potassium iodide, the iodine content of the compound iodine solution is 2%, and the concentration of iodine is 60mg/mL. The neutralizing solution is 20mL of the vitamin C solution, wherein the vitamin C content is 2%. The invention overcomes the defect that iodine can stimulate and erode mucosa of upper digestive tracts. According to the invention, the standardization kitset comprising the compound iodine solution staining solution and vitamin C solution neutralization solution is convenient to use, can neutralize iodine staining in time, relieves discomfort symptomsof patients after iodine staining in time, and improves compliance of the patients, so that the detection rate of esophageal squamous dysplasia and canceration is greatly increased.

The invention discloses a deep learning-based digestive tract mucosa staining detection system and method. The system comprises an image acquisition device, a host, an intelligent prompt device, a staining device and a staining action capture device, the output end of the image collecting device is connected with the input end of the host, the output end of the host is connected with the input end of the intelligent prompting device, the output end of the intelligent prompting device is connected with the input end of the staining device, and the staining output end of the staining device outputs staining medicine; and the signal output end of the staining device outputs a staining action signal to the staining action capturing device. On the basis of following a clinical guide or expert consensus for screening the early cancer of the digestive tract, the system and method can be suitable for basic medical institutions and most of existing gastrointestinal endoscopes, and can guide, supervise and urge doctors to do digestive endoscopy examination and improve the examination and diagnosis level and efficiency of the doctors.

The invention provides a medical image processing method and device, a terminal and a storage medium. The method comprises the following steps of: acquiring a blood vessel segmentation image corresponding to an esophageal background mucosa staining and magnifying image; acquiring a corresponding blood-vessel-lack region segmentation image; performing image superposition processing on the blood vessel segmentation image and the blood-vessel-lack region segmentation image to obtain a blood vessel segmentation superposed image; acquiring a background mucosa color characterization image corresponding to the esophageal background mucosa staining and magnifying image; acquiring a background mucosa flatness characterization image corresponding to the esophageal background mucosa staining and magnifying image; performing channel fusion processing on the blood vessel segmentation superposed image, the background mucosa color characterization image and the background mucosa flatness characterization image to obtain an esophageal background mucosa staining and magnifying synthetic image; and determining the infiltration depth of an esophageal marker corresponding to the esophageal background mucosa staining and magnifying image based on the esophageal background mucosa staining and magnifying synthetic image. The accuracy of determining the infiltration depth of an esophageal marker is improved.