Plant plasma membrane fluorescence labeling method and plasma membrane protein positioning and dynamic monitoring method

A fluorescent labeling and plasma membrane technology, applied in the field of plasma membrane staining, can solve the problems of complicated operation, long cycle and high difficulty, and achieve the effect of saving experimental time

Pending Publication Date: 2022-03-25
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows researchers to study plant cells by adding specific molecules called FM 46-464 (a special type of cyanine) dyes that are able to attach themselves on certain parts of their cell walls when exposed to light. These colored particles move along the entirety of the outer layer of the cell wall while carrying water into another part where they don't get stuck well enough. By doing this experimentally, scientists may investigate how different types of chemicals affect these structures inside them more easily than before studying other aspects like DNA replication.

Problems solved by technology

This patented technical problem addressed in this patent relates to developing a simpler and faster way for studying plasmas (a type of fluid containing charged particles) membranes without having complex operations or requiring expensive equipment such as lasers.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant plasma membrane fluorescence labeling method and plasma membrane protein positioning and dynamic monitoring method
  • Plant plasma membrane fluorescence labeling method and plasma membrane protein positioning and dynamic monitoring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Material: transgenic Arabidopsis thaliana with plasma membrane localization protein DsSWEET12 (GenBank accession number: MG737823) (Zhou et al, A Novel Sugar Transporter from Dianthus spiculifolius, DsSWEET12, Affercts Sugar Metabolism and Confers Osmotic and Oxidative Stress Tolerance in Arabidlar Sicis. Molecu. 2018)

[0035] 1. Arabidopsis root hair cell plasma membrane staining;

[0036] Step 1. The transgenic Arabidopsis seeds were sterilized with 70% alcohol for 30 seconds, sterilized with sodium hypochlorite for 3 minutes, washed with sterile water for 5 times, planted on solid 1 / 2 MS medium, and placed at 4°C for 2 days;

[0037] Step 2. Take out the petri dish with seeds and place it vertically in a light incubator for 7 days. The culture temperature is 22°C, the photoperiod is 12h light / 12h dark, and the light intensity is 150μE m -2 the s -1 ;

[0038] Step 3: Use scissors to cut the whole root of Arabidopsis seedlings grown vertically for 7 days in a petr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a plasma membrane fluorescence labeling method of a plant and a plasma membrane protein positioning and dynamic monitoring method, and belongs to the technical field of plasma membrane dyeing. According to the invention, the FM4-64 dye is adopted to dye the plasma membrane structure, and the FM4-64 dye can be embedded in the plasma membrane bimolecular layer and can be dynamically transferred along with the plasma membrane, so that the FM4-64 dye can also be used by subsequent related researches such as endocytosis and transfer of the plasma membrane. And moreover, the fluorescence labeling method disclosed by the invention can be used for carrying out fluorescence labeling on the plasma membrane during genetic transformation or instantaneous transformation, so that the experiment time is saved, and the interference problem of a fluorescence signal does not exist.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap