30 results about "Hyponitrite reductase" patented technology

The present invention discloses functional nitrite reductase as a potential drug target for anti-tubercular drug development. The present invention also relates to the development of an easy method for identification of nitrite in clinical samples as well as its correlation with the severity of the disease. Presence of active as well as dormant / latent stages of Mycobacterium tuberculosis (MTB) could be identified from nitrite in clinical samples like sputum of potential TB patients.

The invention discloses a method for improving denitrification of integrated vertical flow constructed wetlands. The method for improving the denitrification of the integrated vertical flow constructed wetlands comprises the steps that (I) waste water enters a surface conductive filler layer and a middle nonconductive filler layer of a downstream pool in sequence, and ammonia nitrogen in the waste water is oxidized and becomes nitrate nitrogen under the action of the aerobic denitrifying bacteria and nitrifying bacteria; (II) the waste water obtained through the step (I) enters a communication layer of the downstream pool, a communication layer of an upstream pool, a middle nonconductive filler layer of the upstream pool in sequence, and the nitrate nitrogen in the waste water is reduced to nitrogen through a heterotrophic mode under the action of nitrate reductase, nitrite reductase, nitric oxide reductase and nitrous oxide reductase; (III) the water obtained through the step (II) enters a surface conductive filler layer of the upstream pool, and the nitrate nitrogen in the waste water is reduced to nitrogen through a heterotrophic mode under the action of electrochemical autotrophic denitrification; (IV) the denitrified waste water obtained through the step (III) finally flows out through water outlet collecting pipes. The method is simple and convenient to operate and capable of obviously improving the denitrification effect of the integrated vertical flow constructed wetlands.

The invention discloses a method for degrading nitrite in salted fish through an enzymic method. The method comprises the following steps: (1) washing fresh fish or frozen fish, salting the fish with salt, taking out the fish, rinsing with clean water, and draining; (2) adding nitrite reductase into the salted fish, wherein the addition amount of the nitrite reductase is 0.8-1.6g per kg of fish, and the enzymatic activity of the nitrite reductase is 3,500-4,500U / g; (3) airing the fish treated with an enzyme solution on a drying rack for 1-2 hours, putting the fish in a low-temperature heat pump dryer or cold-air drying equipment at the temperature of 28-35 DEG C, and airing the fish until the moisture content of the fish body is 35-45 percent. According to the method, the content of the nitrite generated in the process of processing the salted fish can be effectively reduced, the taste and quality of the salted fish products are kept, and the safety of the salted fish products is guaranteed.

The invention discloses a nitrite-degrading electron donor protein obtained from lactobacillus casei and a preparation method thereof and application. The preparation method comprises the following steps: (1) cultivating and preparing lactobacillus casei subsp.rhamnosus into a seed solution, inoculating the lactobacillus casei subsp.rhamnosus to an induction medium A, performing static induced cultivation to obtain an induced working leavening agent; (2) inoculating the induced working leavening agent to an induction medium B, and performing static induced cultivation to obtain a culture solution containing the electron donor protein for degrading nitrite; (3) centrifuging the culture solution containing the electron donor protein for degrading nitrite, performing cleaning and centrifugation, and obtaining bacteria; (4) adding an electron donor protein buffer solution to the bacteria, adding lysozyme, performing cell disruption after uniform mixing, obtaining supernatant of a centrifuged cell disruption solution, wherein the supernatant is an electron donor crude protein fluid for degrading nitrite; and obtaining the electron donor protein by purifying the crude protein fluid. A mixed solution of the electron donor protein and nitrite reductase can quickly degrade nitrite.

The invention discloses a transcription factor derived from corn and new use of a coding gene thereof. The new use provided by the invention is a method using a coding nucleic acid molecule of ZmNLP1;1 to cultivate a transgenic plant. The method is a method for cultivating the transgenic plant with at least one characters of the following 1)-4): 1) aboveground biomass is higher than that of a receptor plant; 2) after induction of a nitrate, the nitrate transport protein gene expression is higher than that of the receptor plant; 3) after induction of the nitrate, nitrate reductase gene expression is higher than that of the receptor plant; and 4) after induction of the nitrate, nitrite reductase gene expression is higher than that of the receptor plant; and the method comprises a step for introducing a ZmNLP1;1 gene into the receptor plant; and the ZmNLP1;1 gene codes a protein shown in SEQ ID No.2. Experiments show that, in the condition of using nitric nitrogen (NO<3->) as a sole nitrogen source, the aboveground biomass and the total biomass of arabidopsis transgenic with the ZmNLP1;1 gene are increased significantly compared with that of receptor arabidopsis.

The invention discloses an aspergillus parasiticus and its application in the preparation of nitrite reductase, nitrite reductase gene and genetic engineering bacteria containing nitrite reductase. The preservation number of aspergillus parasiticus is CCTCC M2013662. The invention provides an aspergillus parasiticus with high efficiency, low energy consumption, good effect on nitrite degradation, fast and reliable degradation. And the aspergillus parasiticus can be well applied in the preparation of nitrite reductase, nitrite reductase gene preparation and genetically engineered bacteria containing nitrite reductase.