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A kind of electron donor protein of Lactobacillus casei degrading nitrite and its preparation method and application

A technology of Lactobacillus casei and electron donor, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of inappropriateness and toxicity of Pseudomonas, and achieve the goal of not being easily lost , stable in quantity and quality, and good commercial prospects

Active Publication Date: 2018-10-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Pseudomonas may be toxic and not suitable for reducing nitrite in food, the electron donor protein extracted from nitrite-degraded lactic acid bacteria Lactobacillus casei has not been reported

Method used

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  • A kind of electron donor protein of Lactobacillus casei degrading nitrite and its preparation method and application
  • A kind of electron donor protein of Lactobacillus casei degrading nitrite and its preparation method and application
  • A kind of electron donor protein of Lactobacillus casei degrading nitrite and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0036] 1, a kind of preparation method of the electron donor protein of Lactobacillus casei degradation nitrite, comprises following steps and process condition:

[0037](1) Place the freeze-dried powder of Lactobacillus casei subsp. China Industrial Microorganism Culture Collection Management Center, numbered CICC 6013, named Lactobacillus casei subsp.rhamnosus 6013 (abbreviated as LCR 6013).

[0038] (2) LCR 6013 seed solution was inoculated into induction medium A at 5% by volume, and cultured for static induction at 37° C. for 24 hours to obtain an induction working starter. The formula of the induction medium A is: containing the inducer NaNO 2 MRS medium with a concentration of 10 μg / mL.

[0039] (3) Inoculate the induction working starter into the induction medium B at a volume percentage of 5%, and induce statically at 37° C. for 24 hours, the culture solution containing the electron donor protein for degrading nitrite. The formulation of the induction medium B is: ...

Embodiment 2

[0051] 1, a kind of preparation method of the electron donor protein of Lactobacillus casei degradation nitrite, comprises following steps and process condition:

[0052] (1) The freeze-dried powder of Lactobacillus casei subsp. rhamnose was placed in MRS medium, and after static culture at 30° C. for 36 hours, a seed solution was prepared.

[0053] (2) LCR 6013 seed solution was inoculated into induction medium A at 10% by volume, and incubated at 40° C. for 18 hours to obtain an induction working starter. The formula of the induction medium A is: containing the inducer NaNO 2 MRS medium with a concentration of 5 μg / mL.

[0054] (3) Inoculate the induction working starter into the induction medium B at a volume percentage of 15%, and induce statically at 30° C. for 24 hours, the culture solution containing the electron donor protein for degrading nitrite. The formulation of the induction medium B is: containing the inducer NaNO 2 MRS medium with a concentration of 20 μg / mL...

Embodiment 3

[0066] 1, a kind of preparation method of the electron donor protein of Lactobacillus casei degradation nitrite, comprises following steps and process condition:

[0067] (1) Place the lyophilized powder of Lactobacillus casei subsp. rhamnose in the MRS medium, and culture it statically at 37° C. for 24 hours to make a seed solution;

[0068] (2) LCR 6013 seed solution was inoculated into induction medium A at 1% volume percentage, and induced and cultured statically at 40° C. for 24 hours to obtain an induction working starter. The formula of the induction medium A is: containing the inducer NaNO 2 MRS medium with a concentration of 5 μg / mL.

[0069] (3) Inoculate the induction working starter into the induction medium B at a volume percentage of 10%, and induce statically at 30° C. for 24 hours, the culture solution containing the electron donor protein for degrading nitrite. The formulation of the induction medium B is: containing the inducer NaNO 2 MRS medium with a con...

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Abstract

The invention discloses a nitrite-degrading electron donor protein obtained from lactobacillus casei and a preparation method thereof and application. The preparation method comprises the following steps: (1) cultivating and preparing lactobacillus casei subsp.rhamnosus into a seed solution, inoculating the lactobacillus casei subsp.rhamnosus to an induction medium A, performing static induced cultivation to obtain an induced working leavening agent; (2) inoculating the induced working leavening agent to an induction medium B, and performing static induced cultivation to obtain a culture solution containing the electron donor protein for degrading nitrite; (3) centrifuging the culture solution containing the electron donor protein for degrading nitrite, performing cleaning and centrifugation, and obtaining bacteria; (4) adding an electron donor protein buffer solution to the bacteria, adding lysozyme, performing cell disruption after uniform mixing, obtaining supernatant of a centrifuged cell disruption solution, wherein the supernatant is an electron donor crude protein fluid for degrading nitrite; and obtaining the electron donor protein by purifying the crude protein fluid. A mixed solution of the electron donor protein and nitrite reductase can quickly degrade nitrite.

Description

technical field [0001] The invention relates to the field of biological products, and relates to a method for preparing an electron donor protein for degrading nitrite in Lactobacillus caseis subsp. rhamnosus 6013 (abbreviated as LCR 6013). Background technique [0002] Nitrite is a potential carcinogen, which is easy to accumulate in the process of vegetable fermentation, bringing potential food safety problems to the product, and excessive intake of nitrite can induce methemoglobinemia, so strict control of nitrite in food The salt content is very important. Many studies have shown that nitrite is the precursor of nitrosamines, which are strong carcinogens that can induce various cancers in the digestive system, such as gastric cancer, intestinal cancer and liver cancer. In view of a series of problems such as the possibility of excessive nitrite pollution in food, the potential food safety problems of nitrite in meat products, and the potential food safety problems cause...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C07K2/00A23L5/20C02F3/00C12R1/245C02F101/16C02F103/20
Inventor 刘冬梅陈浩周劲松陈舒然张馨月
Owner SOUTH CHINA UNIV OF TECH
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