Method for regulating microbial denitrifying florae on basis of combination of powdered activated carbon and organic matter

A technology for powdered activated carbon and organic matter, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that the anammox process cannot run stably, it is difficult to maintain the stable operation of the reactor, and it is difficult to maintain a high biological concentration. and other problems, to achieve the effect of improving the ability of denitrification and degrading organic matter, the cost is green and pollution-free, and the removal rate of nitrogen is improved.

Pending Publication Date: 2018-06-19
SHANDONG UNIV
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Problems solved by technology

[0004] Anammox bacteria grow slowly and have low cell productivity, so it is difficult to maintain a high bioconcentration. Even if the anammox function is established after several months of operation, it is still difficult to maintain due to the sensitivity of anammox bacteria to environmental conditions The stable operation of the reactor, in the process of industrial application, is also difficult to maintain long-term stable operation because of the poor adaptability of the anammox bacteria group to the actual sewage
[0005] At the same time, anammox bacteria are easily inhibited by organic matter, which leads to unstable operation of the anammox process

Method used

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  • Method for regulating microbial denitrifying florae on basis of combination of powdered activated carbon and organic matter
  • Method for regulating microbial denitrifying florae on basis of combination of powdered activated carbon and organic matter
  • Method for regulating microbial denitrifying florae on basis of combination of powdered activated carbon and organic matter

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Embodiment 1

[0042] A method for regulating microbial denitrification flora based on powdered activated carbon combined with organic matter, comprising the following steps:

[0043](1) Rinse the activated carbon repeatedly with tap water, bake it in an oven at 50°C for 12 hours, take it out and grind it into powder, and then add it to the SMABR. : 3 put into SMABR reactor, active anaerobic ammonium oxidation activated sludge and anaerobic granular sludge mixed sludge moisture content ≥ 99wt%, activated anaerobic granular sludge sludge is taken from starch plant anaerobic sewage treatment device discharge The sludge is obtained after filtering and washing; the activated anaerobic ammonium oxidation sludge is obtained by enrichment and cultivation in an anaerobic ammonium oxidation reactor.

[0044] (2) The top of the SAMBR reactor uses a peristaltic pump to feed the wastewater to be treated. At the same time, glucose and sodium acetate with a molar ratio of 1:1 are added to the wastewater, ...

Embodiment 2

[0058] A method based on powdered activated carbon combined with organic matter to regulate microbial denitrification flora, as described in Example 1, the difference is:

[0059] Step (1) The volume ratio of activated anaerobic ammonium oxidation activated sludge to anaerobic granular sludge is 1:4.

[0060] Step (2) The top of the SAMBR reactor uses a peristaltic pump to feed the waste water to be treated. At the same time, glucose and sodium acetate with a molar ratio of 1:1 are added to the waste water. The amount of organic matter added keeps the COD in the waste water at 60mg / L. After the wastewater to be treated is introduced, the concentration of powdered activated carbon is 1200mg / L; the water inflow speed is controlled by controlling the speed of the peristaltic pump, so that the hydraulic retention time in the reactor is 50h. Nitrogen was introduced into the SMABR reactor every 12 hours for 20 min, and the aeration intensity was 5 mL / (L·min) to maintain the anaerobi...

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Abstract

The invention relates to a method for regulating microbial denitrifying florae on the basis of combination of powdered activated carbon and organic matter. According to the method, after activated anammox sludge, anaerobic granular sludge and activated carbon powder are added to an SAMBR, to-be-treated wastewater is introduced, the organic matter is added, and the microbial denitrifying florae areregulated. The method has the following advantages: microbial community structure in the anammox process can be domesticated effectively, and nitrogen-containing wastewater containing the organic matter can be removed from the domesticated activated sludge; proteobacteria becomes dominant phylum, relative content of predicted genes related to nitrite reductase, nitric oxide reductase and other enzymes throughout a denitrification pathway in a nitrogen circulation pathway is increased remarkably, the whole denitrification process is enhanced, and the anammox process can adapt to the wastewatercontaining the organic matter.

Description

technical field [0001] The invention relates to a method for regulating microbial denitrification flora based on powdered activated carbon combined with organic matter, and belongs to the technical field of wastewater microbial treatment. Background technique [0002] In recent years, compared with the traditional sewage treatment process based on nitrification-denitrification, the new sewage denitrification process centered on anaerobic ammonium oxidation has become a national waste water denitrification process due to its advantages such as no need for additional carbon sources, no need to expose a large amount of air, and low infrastructure costs. Research hotspots in the field of water environmental protection. Anaerobic ammonium oxidation means that under anaerobic or anoxic conditions, microorganisms directly use NH4+-N as the electron donor, NO2--N as the electron acceptor, and convert NH 4+ -N and NO 2- The biological process of converting -N to N2, the anaerobic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/28C12N1/20C12R1/37C12R1/01C02F101/16C02F101/30C02F103/34C02F103/36C02F103/06
CPCC02F3/28C02F3/34C02F2101/16C02F2101/30C02F2103/06C02F2103/343C02F2103/365C12N1/20
Inventor 倪寿清葛成浩
Owner SHANDONG UNIV
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